Reviewed, UniProtKB/Swiss-Prot A4SME2 (PYRD_AERS4)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.3.1
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	ASA_1993

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + O2 = orotate + H2O2. HAMAP MF_00225
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6. HAMAP MF_00225
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Dihydroorotate dehydrogenase HAMAP MF_00225		PRO_1000024148
Sites
	Active site	175	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4SME2-1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: EC003C32D9230911	FASTA	336	36,411
	10 20 30 40 50 60 MLYPLARHFL FKFNPEQAHD LSIKYLPRLL GTPLDCFFRH SLPKRPVTVM GLYFANPVGL 70 80 90 100 110 120 AAGLDKDGEC IDAFGAMGFG FIEVGTVTPK PQSGNDKPRL FRVIPAEGII NRMGFNNKGV 130 140 150 160 170 180 DHLVAQVKKA KYQGVIGINI GKNKDTPIEQ GKDDYLICMD KVYDHAGYIA VNISSPNTPG 190 200 210 220 230 240 LRSLQYGDAL DELLAALKVR QQELAAQYKK YVPLAVKIAP DLSLDEINQV AASLIKNGID 250 260 270 280 290 300 GVIATNTTLD REMIYDMPHA GEAGGLSGRP LQHKSTEVIR QLAKALDGAL PIIGVGGIDS 310 320 330 AMAAREKLAA GASLVQIYSG FIYKGPSLVK EIVTHI

References

[1]

"The genome sequence of Aeromonas salmonicida subsp. salmonicida A449." Reith M.E., Singh R.K., Curtis B., Boyd J., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J., Johnson S., Brown L. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000644 Genomic DNA. Translation: ABO90064.1.
RefSeq	YP_001141812.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4996284.
GenomeReviews	Gene locus ASA_1993 in contig CP000644_GR.
KEGG	asa:ASA_1993.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A4SME2. NAEQQGG.
Family and domain databases
HAMAP	MF_00225. [Tree]
InterPro	IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_AERS4

Accession

Primary (citable) accession number: A4SME2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 15, 2007
Last modified:	June 16, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)