A4SMA4 (SYT_AERS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	ASA_1953

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	642 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subunit structure	Homodimer By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 642	642	Threonine--tRNA ligase HAMAP MF_00184		PRO_1000020334
Regions
	Region	243 – 534	292	Catalytic HAMAP MF_00184
Sites
	Metal binding	334	1	Zinc; catalytic By similarity
	Metal binding	385	1	Zinc; catalytic By similarity
	Metal binding	511	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4SMA4 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: A25D5378E1234E27	FASTA	642	72,916
	10 20 30 40 50 60 MPIITLPDGS QRQFAHSVSV MDVAADIGPG LAKACIAGRV NGELVDACEL IEADASLAII 70 80 90 100 110 120 TAKDEEGLDI LRHSCAHLLG HAIKQLWPQT KMAIGPVIDN GFYYDVDLDR TLTDDDLAAL 130 140 150 160 170 180 EERMLALAAK DYDVIKKKVS WQEARDVFEA RGETYKVEIL DQNIARDDQP GLYHHEEYVD 190 200 210 220 230 240 MCRGPHVPNM RHCHHFKLQK MSGAYWRGDS NNKMLQRIYG TAWADKKQLK GYLQRLEEAS 250 260 270 280 290 300 KRDHRKIGKQ LDLYHMQEEA PGMVFWHNDG WTIFRELETF IRGKLKEYDY QEVKGPFMMD 310 320 330 340 350 360 RVLWERSGHW EKYAQAMFTT QSENREYAIK PMNCPGHVQI FNQGLKSYRD LPLRMAEFGS 370 380 390 400 410 420 CHRNEPSGSL HGLMRVRGFT QDDAHIFCTE EQIMEEVSAC IRMVYDVYGT FGFENIVVKL 430 440 450 460 470 480 STRPEQRIGS DEAWDRAEAA LAEALVLNGL KYDLQPGEGA FYGPKIEFTL HDCLDRAWQC 490 500 510 520 530 540 GTVQLDFALP GRLGATYVGE DNERHVPVMI HRAILGSLER FIGILTEEYA GLFPTWLAPT 550 560 570 580 590 600 QAVVMNITDN QADYAVKVAK ALNDAGLRAK ADLRNEKIGF KIREHTLKRV PFMLVCGDKE 610 620 630 640 VEAGKIAVRT RKGADLGTYP VEELVALLTQ EVQTRGQKKV EE

References

[1]

"The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen." Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L. BMC Genomics 9:427-427(2008) [PubMed: 18801193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A449.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000644 Genomic DNA. Translation: ABO90026.1.
RefSeq	YP_001141774.1. NC_009348.1.
3D structure databases
ProteinModelPortal	A4SMA4.
SMR	A4SMA4. Positions 2-642.
ModBase	Search...
Protein-protein interaction databases
STRING	A4SMA4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4995437.
GenomeReviews	Gene locus ASA_1953 in contig CP000644_GR.
KEGG	asa:ASA_1953.
PATRIC	20790280. VBIAerSal2987_1936.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0441.
HOGENOM	HBG352811.
OMA	KVYQSAL.
PhylomeDB	A4SMA4.
ProtClustDB	PRK00413.
Enzyme and pathway databases
BioCyc	ASAL382245:ASA_1953-MONOMER.
Family and domain databases
HAMAP	MF_00184. Thr_tRNA_synth. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_ferredoxin-type. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-synth_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KO	K01868.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF81271. TGS-like. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_AERS4

Accession

Primary (citable) accession number: A4SMA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 15, 2007
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program