Reviewed, UniProtKB/Swiss-Prot A4SM23 (GUAA_AERS4)

Names and origin

Protein names

Recommended name:
    GMP synthase [glutamine-hydrolyzing]
    EC=6.3.5.2
Alternative name(s):
    Glutamine amidotransferase
    GMP synthetase

Gene names

Name:	guaA
Ordered Locus Names:	ASA_1869

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity.
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00344
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00344
Subunit structure	Homodimer By similarity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain. Contains 1 GMP-binding domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 526	526	GMP synthase [glutamine-hydrolyzing] HAMAP MF_00344		PRO_1000120204
Regions
	Domain	9 – 208	200	Glutamine amidotransferase type-1
	Domain	240 – 402	163	GMP-binding
	Nucleotide binding	236 – 242	7	ATP By similarity
Sites
	Active site	86	1	Nucleophile By similarity
	Active site	182	1	By similarity
	Active site	184	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4SM23-1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: FDED7A9DA6D1BD54	FASTA	526	58,513
	10 20 30 40 50 60 MTKDIHQHRI LILDFGSQYT QLIARRVREI GVYCELWAWD VTEEQIREFN PSGIILSGGP 70 80 90 100 110 120 ESVTEAGSPR APEYVFNAGV PVFGICYGMQ TMAEQLGGKV QSSTEREFGY AQVEVLGQTN 130 140 150 160 170 180 ALLRSIEDAI APNGNALLDV WMSHGDKVTA IPADFTTIAQ TATCPHAAMA CESKHFYGVQ 190 200 210 220 230 240 FHPEVTHTRQ GARMLEHFVK DICGCECLWT PATIIDDAVA RIREQVGDDE VILGLSGGVD 250 260 270 280 290 300 SSVVAMLVHR AIGDRLTCVF VDNGLLRLNE GEQVMEMFGD HFGLNIIKVE AEERFLSALA 310 320 330 340 350 360 GEDEPEAKRK IIGRVFVEVF DDEAKKLKNA RWLAQGTIYP DVIESAASKT GKAHVIKSHH 370 380 390 400 410 420 NVGGMPAEMK MGLVEPLREL FKDEVRRVGL ELGLPYDMLY RHPFPGPGLG VRVLGEVKKE 430 440 450 460 470 480 YCDILRRADA VFIEELRKAD LYNQVSQAFA VFLPVRSVGV MGDGRKYDWV IALRAVETID 490 500 510 520 FMTAHWAHLP YDFLGHVSNR IINEINGISR VVYDVSGKPP ATIEWE

References

[1]

"The genome sequence of Aeromonas salmonicida subsp. salmonicida A449." Reith M.E., Singh R.K., Curtis B., Boyd J., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J., Johnson S., Brown L. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000644 Genomic DNA. Translation: ABO89945.1.
RefSeq	YP_001141693.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4SM23.
Genome annotation databases
GeneID	4997379.
GenomeReviews	Gene locus ASA_1869 in contig CP000644_GR.
KEGG	asa:ASA_1869.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	DQVMEMF.
Family and domain databases
HAMAP	MF_00344. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR018318. tRNA_MeTrfase-like. [Graphical view]
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF03054. tRNA_Me_trans. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR00884. guaA_Cterm. 1 hit. TIGR00888. guaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAA_AERS4

Accession

Primary (citable) accession number: A4SM23

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	May 15, 2007
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)