A4SKE9 (MAO1_AERS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NAD-dependent malic enzyme
Short name=NAD-ME
EC=1.1.1.38

Gene names

Name:	maeA
Ordered Locus Names:	ASA_1264

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity. HAMAP MF_01619
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 564	564	NAD-dependent malic enzyme HAMAP MF_01619		PRO_1000069526
Sites
	Active site	104	1	Proton donor By similarity
	Active site	175	1	Proton acceptor By similarity
	Metal binding	246	1	Divalent metal cation By similarity
	Metal binding	247	1	Divalent metal cation By similarity
	Metal binding	270	1	Divalent metal cation By similarity
	Binding site	157	1	NAD By similarity
	Binding site	270	1	NAD By similarity
	Binding site	417	1	NAD By similarity
	Site	270	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4SKE9 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 21F79D074BEE625B	FASTA	564	62,521
	10 20 30 40 50 60 MFEDNNQKRP LYIPYAGPAL LETPLLNKGC AFTSEERSSF NLEGLLPQNI ETIEEQAERA 70 80 90 100 110 120 YRQFMAFGND LDKHIYLRNI QDTNETLFYR LLHNHLTEML PVIYTPTVGK ACEEFSNIYR 130 140 150 160 170 180 RARGLFISYP DKDRIDDMLQ NATKQNVKVI VVTDGERILG LGDQGIGGMG IPIGKLSLYT 190 200 210 220 230 240 ACGGISPAYC LPVVLDVGTN NQQLLSDPFY MGWRNPRISG EEYAEFVDAF IQAVKRRWPD 250 260 270 280 290 300 ILLQFEDFAQ NNAMPLLNRY KNELCCFNDD IQGTAAVTLG SLIAACKASG AKLSEKRVAF 310 320 330 340 350 360 LGAGSAGCGI AEQIVAQMKA EGLTDAQARG RVFMVDRFGL ITDKIPNQLD FQRRLSQPVG 370 380 390 400 410 420 RIADWPVGDN ISLLEVMEHG RPDILIGVSG QPGLFTEEVV KTMHKHCTRP IIFPLSNPTS 430 440 450 460 470 480 RVEATPADLI RWTDGQALVA TGSPFAPVEY KGKRYVIAQC NNSFIFPGIG LGVIASGATR 490 500 510 520 530 540 VTDAMLMSAS RALAECSPLV KGAEGSLLPD LADIHQVSRY IAKMVAKTAM LQGKAVQTPD 550 560 EVIDQAIEAN FWRPEYRRYR RTSF

References

[1]

"The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen." Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L. BMC Genomics 9:427-427(2008) [PubMed: 18801193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A449.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000644 Genomic DNA. Translation: ABO89371.1.
RefSeq	YP_001141119.1. NC_009348.1.
3D structure databases
ProteinModelPortal	A4SKE9.
ModBase	Search...
Protein-protein interaction databases
STRING	A4SKE9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4998452.
GenomeReviews	Gene locus ASA_1264 in contig CP000644_GR.
KEGG	asa:ASA_1264.
PATRIC	20788910. VBIAerSal2987_1262.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0281.
HOGENOM	HBG289821.
OMA	QDNNETL.
PhylomeDB	A4SKE9.
ProtClustDB	PRK13529.
Enzyme and pathway databases
BioCyc	ASAL382245:ASA_1264-MONOMER.
Family and domain databases
HAMAP	MF_01619. NAD_malic_enz. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD-bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. IPR023667. NAD_malic_enz_proteobac. [Graphical view]
Gene3D	G3DSA:3.40.50.10380. G3DSA:3.40.50.10380. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00027.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PIRSF	PIRSF000106. ME. 1 hit.
PRINTS	PR00072. MALOXRDTASE.
SMART	SM00919. Malic_M. 1 hit. [Graphical view]
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_AERS4

Accession

Primary (citable) accession number: A4SKE9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 15, 2007
Last modified:	December 14, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program