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A4SKA6 (DNLJ_AERS4) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA ligase
EC=6.5.1.2
Alternative name(s):
Polydeoxyribonucleotide synthase [NAD+]
Gene names
Name:ligA
Ordered Locus Names:ASA_1220
OrganismAeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]
Taxonomic identifier382245 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length664 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA By similarity. HAMAP MF_01588

Catalytic activity

NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m). HAMAP MF_01588

Cofactor

Magnesium or manganese By similarity. HAMAP MF_01588

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Contains 1 BRCT domain.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
DNA replication
   LigandMagnesium
Manganese
Metal-binding
NAD
Zinc
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processDNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

DNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintracellular

Inferred from electronic annotation. Source: InterPro

   Molecular functionDNA binding

Inferred from electronic annotation. Source: InterPro

DNA ligase (NAD+) activity

Inferred from electronic annotation. Source: EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 664664DNA ligase HAMAP MF_01588
PRO_0000313105

Regions

Domain586 – 66479BRCT
Nucleotide binding32 – 365NAD By similarity
Nucleotide binding81 – 822NAD By similarity

Sites

Active site1151N6-AMP-lysine intermediate By similarity
Metal binding4071Zinc By similarity
Metal binding4101Zinc By similarity
Metal binding4251Zinc By similarity
Metal binding4311Zinc By similarity
Binding site1131NAD By similarity
Binding site1361NAD By similarity
Binding site1731NAD By similarity
Binding site2891NAD By similarity
Binding site3131NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
A4SKA6 [UniParc].

Last modified May 15, 2007. Version 1.
Checksum: 671F071612DC0622

FASTA66472,103
        10         20         30         40         50         60 
MSETLSRHRQ LCDLLTEYGH QYYVLDNPTV PDAEYDRLMR ELIAIESEHP ELKTSASPSV 

        70         80         90        100        110        120 
RVGGQPLTAF RQVRHEIPML SLDNVFSSEE LQAFEQRMKD RLKREISFTF CCEPKLDGLA 

       130        140        150        160        170        180 
VSLLYVDGQL VQAATRGDGA TGEEITENVR TIKAIPLSLR GSDWPARLEV RGEVFMPKAG 

       190        200        210        220        230        240 
FEAMNAKALA AGEKVFVNPR NAAAGSLRQL DSRITASRPL NFYAYGVGVG GDQLGDSHFG 

       250        260        270        280        290        300 
RLCQLKAWGL PMSPEVKLKE GAAGCQAFHD DILARRGELA YEIDGVVYKV DAIPLQEELG 

       310        320        330        340        350        360 
FVARAPRWAT AHKFPAQEEM TLLENVEFQV GRTGAVTPVA KLKPVFVGGV TISNATLHNA 

       370        380        390        400        410        420 
DEIERLGVMI GDTVIVRRAG DVIPQIVAVV EAQRPVDARE ILFPTQCPVC GSALERLEGE 

       430        440        450        460        470        480 
VVTRCSGGLF CEAQRKEAIK HFAARRAMDV DGLGDKIVEQ LVDKGLVKTP ADLFSLNAIQ 

       490        500        510        520        530        540 
LAGLERMGQK SALNLVAAID AARSTTLPRF LFALGIREVG EATALNLANH FLTLDALRAA 

       550        560        570        580        590        600 
SVEQLLEVAD VGDIVAKHVY YFLRQPHNIE VLAAGIHWPA IEKKEASEQP FAGKTFVLTG 

       610        620        630        640        650        660 
TLNLLSRNDA KAALQALGAK VAGSVSAKTD VLVAGEAAGS KLAKAQELGI TIWTEEQLQA 


ALQS

« Hide

References

[1]"The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen."
Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L.
BMC Genomics 9:427-427(2008) [PubMed: 18801193] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: A449.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000644 Genomic DNA. Translation: ABO89328.1.
RefSeqYP_001141076.1. NC_009348.1.

3D structure databases

ProteinModelPortalA4SKA6.
SMRA4SKA6. Positions 6-580.
ModBaseSearch...

Protein-protein interaction databases

STRINGA4SKA6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4996062.
GenomeReviewsGene locus ASA_1220 in contig CP000644_GR.
KEGGasa:ASA_1220.
PATRIC20788818. VBIAerSal2987_1216.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0272.
HOGENOMHBG620317.
OMAENVRTIR.
PhylomeDBA4SKA6.
ProtClustDBCLSK2516575.

Enzyme and pathway databases

BioCycASAL382245:ASA_1220-MONOMER.

Family and domain databases

HAMAPMF_01588. DNA_ligase_A.
[Tree]
InterProIPR001357. BRCT.
IPR018239. DNA_ligase_AS.
IPR004150. DNA_ligase_OB.
IPR001679. DNAligase.
IPR013839. DNAligase_adenylation.
IPR013840. DNAligase_N.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR010994. RuvA_2-like.
IPR004149. Znf_DNAligase_C4.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KOK01972.
PfamPF00533. BRCT. 1 hit.
PF01653. DNA_ligase_aden. 1 hit.
PF03120. DNA_ligase_OB. 1 hit.
PF03119. DNA_ligase_ZBD. 1 hit.
[Graphical view]
PIRSFPIRSF001604. LigA. 1 hit.
SMARTSM00292. BRCT. 1 hit.
SM00278. HhH1. 4 hits.
SM00532. LIGANc. 1 hit.
[Graphical view]
SUPFAMSSF52113. BRCT. 1 hit.
SSF50249. Nucleic_acid_OB. 1 hit.
SSF47781. RuvA_2_like. 1 hit.
TIGRFAMsTIGR00575. Dnlj. 1 hit.
PROSITEPS50172. BRCT. 1 hit.
PS01055. DNA_LIGASE_N1. 1 hit.
PS01056. DNA_LIGASE_N2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLJ_AERS4
AccessionPrimary (citable) accession number: A4SKA6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 15, 2007
Last modified: December 14, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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