A4SK61 (ISPE_AERS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	ASA_1172

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000335697
Regions
	Nucleotide binding	95 – 105	11	ATP Potential
Sites
	Active site	11	1	By similarity
	Active site	137	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4SK61 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: E1ED98C50FCF5A70	FASTA	289	31,782
	10 20 30 40 50 60 MATIRWPAPA KLNLFLNVNG RRPDGYHELQ TLFIFLDHGD WLEFEPLPDT DQLTLSPAIP 70 80 90 100 110 120 GVPDEQNLII RAARLLQARL PSPQGAHIRL EKLLPMGGGI GGGSSDAATT LVALNHLWQA 130 140 150 160 170 180 GLGEDELAEL GVQLGADVPV FVRGRAAFAE GVGERLQPVE LPGAWYLVLK PDCHVATAAV 190 200 210 220 230 240 FQDPDLPRAT PKMAFHNLLE GEWKNDCELL VKKRYPEVAN ALGWLLEYAP SRMTGTGACV 250 260 270 280 FAQFEDEKAA REVLAKVPKR WDGFVAKGEN ISPLFVTLQQ ISDWDIAKR

References

[1]

"The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen." Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L. BMC Genomics 9:427-427(2008) [PubMed: 18801193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A449.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000644 Genomic DNA. Translation: ABO89283.1.
RefSeq	YP_001141031.1. NC_009348.1.
3D structure databases
ProteinModelPortal	A4SK61.
SMR	A4SK61. Positions 5-274.
ModBase	Search...
Protein-protein interaction databases
STRING	A4SK61.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4994896.
GenomeReviews	Gene locus ASA_1172 in contig CP000644_GR.
KEGG	asa:ASA_1172.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	LNLFLHV.
PhylomeDB	A4SK61.
ProtClustDB	PRK00343.
Enzyme and pathway databases
BioCyc	ASAL382245:ASA_1172-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_AERS4

Accession

Primary (citable) accession number: A4SK61

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 15, 2007
Last modified:	November 16, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program