Reviewed, UniProtKB/Swiss-Prot A4SK35 (ASTD_AERS4)

Names and origin

Protein names

Recommended name:
    N-succinylglutamate 5-semialdehyde dehydrogenase
    EC=1.2.1.71
Alternative name(s):
    Succinylglutamic semialdehyde dehydrogenase
      Short name=SGSD

Gene names

Name:	astD
Ordered Locus Names:	ASA_1136

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	489 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate By similarity.
Catalytic activity	N-succinyl-L-glutamate 5-semialdehyde + NAD+ + H2O = N-succinyl-L-glutamate + NADH. HAMAP MF_01174
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 4/5. HAMAP MF_01174
Sequence similarities	Belongs to the aldehyde dehydrogenase family. AstD subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	succinylglutamate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 489	489	N-succinylglutamate 5-semialdehyde dehydrogenase HAMAP MF_01174		PRO_1000065746
Regions
	Nucleotide binding	223 – 228	6	NAD By similarity
Sites
	Active site	246	1	By similarity
	Active site	280	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4SK35-1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 1C0701C1BF730184	FASTA	489	51,986
	10 20 30 40 50 60 MSHLVQLIDG QWLAGEGKPF ESMDPAKNEV IWQGGAASAS QVDAAVKAAR AAFYHWSDLA 70 80 90 100 110 120 LEDRLAIVRR YADLLGEHKE ALALTIARET GKPLWETRTE VAAMQGKIAI SIRAHDERTG 130 140 150 160 170 180 TVENPMPGAK AFVRHKPHGV VAVFGPYNFP GHLPNGHIVP ALIAGNTVVF KPSELTPMVA 190 200 210 220 230 240 EAMLKIWQEA GLPKGVLNLV QGEVETGKAL AGNPDIDGLF FTGSSRTGHF LHQQFAGQPG 250 260 270 280 290 300 KILALEMGGN NPLIVKDVSD VDGAVHAIVQ SAFITSGQRC TCSRRLFVER GARGDALVKR 310 320 330 340 350 360 LVEVVGQIKV GLYDAADQPF MGAMISEKAA LGMVAAQANL QQLGGESLLT LKHLEAGTGF 370 380 390 400 410 420 VSPGIIDVTA IGALPDEEYF GPLLQLIRYD DFDAAIDQGN ATSFGLSAGL LGDNEADWQH 430 440 450 460 470 480 FFKRIRAGIV NWNKPITGAS SAAPFGGIGA SGNHRASAYY AADYCAYPVA SVEDSKAAMP DQLSPGLTF

References

[1]

"The genome sequence of Aeromonas salmonicida subsp. salmonicida A449." Reith M.E., Singh R.K., Curtis B., Boyd J., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J., Johnson S., Brown L. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000644 Genomic DNA. Translation: ABO89257.1.
RefSeq	YP_001141005.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4SK35.
Genome annotation databases
GeneID	4994861.
GenomeReviews	Gene locus ASA_1136 in contig CP000644_GR.
KEGG	asa:ASA_1136.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IRAHDER.
Family and domain databases
HAMAP	MF_01174. [Tree]
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR017649. SuccinylGlu_semiald_DH_AstD. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR03240. arg_catab_astD. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASTD_AERS4

Accession

Primary (citable) accession number: A4SK35

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 15, 2007
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)