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A4SJW8 (SYL_AERS4) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:ASA_1068
OrganismAeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]
Taxonomic identifier382245 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas

Protein attributes

Sequence length858 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 858858Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009287

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif618 – 6225"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6211ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4SJW8 [UniParc].

Last modified May 15, 2007. Version 1.
Checksum: 4D1851864482DA11

FASTA85896,439
        10         20         30         40         50         60 
MQEQYVPQSI EPAVQKHWDA KKTFKAVEKV DKEKFYCLSM FPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VISRYQRLNG KNVLQPIGWD AFGLPAENAA VKNNTAPAPW TYENIEYMKN QLKMLGLGYD 

       130        140        150        160        170        180 
WDRELATCKP DYYRWEQWFF TKLYEKGLVY KKTSSVNWCP NDMTVLANEQ VVDNCCWRCD 

       190        200        210        220        230        240 
TPVEQKEIPQ WFIKITDYAE ELLNDIDNLE GWPEMVKTMQ RNWIGRSEGV NISFAIEGQA 

       250        260        270        280        290        300 
EQLEVYTTRP DTFMGVTYVG IAAGHPLALQ AAATNPGLAA FIEECKNTKV AEAELATMEK 

       310        320        330        340        350        360 
KGMATGLYAI HPLDGRKVPV WVANFVLMNY GTGAVMAVPG HDQRDHEFAT KYGLDIKAVI 

       370        380        390        400        410        420 
KPADGEVDVS DAAYTEKGVL FASGEFDGLD FQGAFDAIAN KLEALGHGKR TVNFRLRDWG 

       430        440        450        460        470        480 
VSRQRYWGAP IPMLTLADGT VVPTPEDQLP VLLPEDVVMD GIQSPIKADA EWAKTTYNGQ 

       490        500        510        520        530        540 
EAFRETDTFD TFMESSWYYA RYCSPDYDKG MLDPAAANHW LPVDQYIGGI EHACMHLLYA 

       550        560        570        580        590        600 
RFFHKLLRDA GLVNSDEPFK RLLCQGMVLA DAFYYKDEKG GNVWVSPTDV KVERDEKGRI 

       610        620        630        640        650        660 
TKAIDNDGRE VIHSGMTKMS KSKNNGIDPQ LMVERYGADT VRLFMMFASP AEMTLEWSDS 

       670        680        690        700        710        720 
GVEGAQRFLR RLWRLTFEHV SAGAVPALDV AALTSEQKAV RRELHKTIAK VSDDVGRRQT 

       730        740        750        760        770        780 
FNTAIAAIME LMNNLAKLGS DEQDRALMQE ALETVVVMLS PITPHIGFEL WKMLGKGDDV 

       790        800        810        820        830        840 
DHATWPVADE AAMVETEKLV VVQINGKMRG KLTVPAEISQ ADVEKLAMAD ASVQKFTDGL 

       850 
TVRKVIYVPG KLLNIVAN 

« Hide

References

[1]"The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen."
Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L.
BMC Genomics 9:427-427(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: A449.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000644 Genomic DNA. Translation: ABO89190.1.
RefSeqYP_001140938.1. NC_009348.1.

3D structure databases

ProteinModelPortalA4SJW8.
SMRA4SJW8. Positions 228-412.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING382245.ASA_1068.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABO89190; ABO89190; ASA_1068.
GeneID4998256.
KEGGasa:ASA_1068.
PATRIC20788496. VBIAerSal2987_1064.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycASAL382245:GJJN-1063-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_AERS4
AccessionPrimary (citable) accession number: A4SJW8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 15, 2007
Last modified: April 16, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries