Reviewed, UniProtKB/Swiss-Prot A4SJP9 (DXS_AERS4)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose-5-phosphate synthase
    EC=2.2.1.7
Alternative name(s):
    1-deoxyxylulose-5-phosphate synthase
      Short name=DXP synthase
      Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	ASA_0990

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	621 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity. HAMAP MF_00315
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP thiamin pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 621	621	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000019002

Sequences

	Sequence		Length	Mass (Da)
	A4SJP9-1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 5511CF8DD5BE3561	FASTA	621	67,486
	10 20 30 40 50 60 MSVDISNFPN LALADTPVEL RSLPFERLPL LCNELREYLL RSVSRSSGHL ASGLGTVELT 70 80 90 100 110 120 VALHYVYNTP FDRLVWDVGH QAYPHKILTG RRDRMQSIRQ KDGLHPFPWR GESEYDVLSV 130 140 150 160 170 180 GHSSTSIGAA LGMAVAAESE GLGRKVVAVI GDGAITAGMA FEALNHAGDV HKDMLVVLND 190 200 210 220 230 240 NEMSISENVG ALNNHLARIM SGKLYTTIRE GGKKVLAGLP PVKELAKRAE EHLKGMVVPG 250 260 270 280 290 300 TLFEELGFNY IGPIDGHDIE ALVETLRNMR NLKDPQLLHV KTKKGKGYEP AEKDPIGYHG 310 320 330 340 350 360 VPKFDPDECT LPKASGGKPS FSAIFGQWLC DMAAKDEKLV GITPAMREGS GLVKFSQQYP 370 380 390 400 410 420 DRYFDVAIAE QHAVTFAAGL AIADKKPVVA IYSSFLQRAY DQVIHDVALQ SLPVLFAIDR 430 440 450 460 470 480 AGLVGADGPT HQGAFDISFL RTVPNMVVMT PSDENECRQM LYTGYQCNQP AAVRYPRGSG 490 500 510 520 530 540 TGIEVQTEMQ VLELGKGRIV RNGKGTAILA FGTLLQQARA AAEALDATLV DMRFVKPMDE 550 560 570 580 590 600 ALVLSLAASH DQFVTIEDNA IMGGAGSAVN ELLMRNKQCK PVLNLGLPDR FVEQGTQEEI 610 620 YALLGLDSTG IQASIEAWLQ A

References

[1]

"The genome sequence of Aeromonas salmonicida subsp. salmonicida A449." Reith M.E., Singh R.K., Curtis B., Boyd J., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J., Johnson S., Brown L. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000644 Genomic DNA. Translation: ABO89121.1.
RefSeq	YP_001140869.1.
3D structure databases
SMR	A4SJP9. Positions 3-619.
ModBase	Search...
Protein-protein interaction databases
STRING	A4SJP9.
Genome annotation databases
GeneID	4998810.
GenomeReviews	Gene locus ASA_0990 in contig CP000644_GR.
KEGG	asa:ASA_0990.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1154.
HOGENOM	HBG571647.
OMA	QRFPDRY.
PhylomeDB	A4SJP9.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR011766. TPP_enzyme_bd_C. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
TIGRFAMs	TIGR00204. dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. False negative. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_AERS4

Accession

Primary (citable) accession number: A4SJP9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 15, 2007
Last modified:	February 9, 2010
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)