A4SJM6 (ACSA_AERS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-coenzyme A synthetase
EC=6.2.1.1

Alternative name(s):
Acetate--CoA ligase
Acyl-activating enzyme

Gene names

Name:	acsA
Ordered Locus Names:	ASA_0967

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	648 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity. HAMAP MF_01123
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 648	648	Acetyl-coenzyme A synthetase HAMAP MF_01123		PRO_1000065266
Sites
	Active site	516	1	By similarity
Amino acid modifications
	Modified residue	608	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4SJM6 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: A14A5C60C1F41248	FASTA	648	71,357
	10 20 30 40 50 60 MSESKVYPVK AHISNGALLD KAGYEAMYRA SVQDPDAFWG EQGKIIDWMK PYTKVKNTSY 70 80 90 100 110 120 DPGHVSIKWY EDGLLNVSAN CLDRHLAQRG DKVAIIWEGD NPAEDRKLTY RELHAEVCKF 130 140 150 160 170 180 SNVLKAQGVH RGDVVCLYMP MVPEAAIAML ACTRIGAVHS IVFGGFSPEA LAGRIIDSGS 190 200 210 220 230 240 SIVITADEGL RGGRPVPLKK NVDEALTNPD TRVSKVIVLK RTGGNVAWHN HRDIWWHEAT 250 260 270 280 290 300 AAASSDCPPE AMNAEDPLFV LYTSGSTGKP KGVLHTTGGY LVYATLTFKY VFDYHEEDIY 310 320 330 340 350 360 WCTADVGWVT GHSYLVYGPL ANGATTIMFE GVPNYPATNR MSQVVDKHQV TILYTAPTAI 370 380 390 400 410 420 RALMAKGRDA VEGTSRQSLR IMGSVGEPIN PEAWEWYYRT IGEERCPIVD TWWQTETGGI 430 440 450 460 470 480 LISPLPGVTD LKPGSATRPF FGVQPALVDN MGEPLEGATE GNLVITDSWP GQMRTVFGDH 490 500 510 520 530 540 ERFEQTYFST FPGRYFTGDG ARRDEDGYYW ITGRVDDVLN VSGHRMGTAE IESALVAHPK 550 560 570 580 590 600 IAEAAVVGVP HEIKGQGIYA YVTLIAGEEP SRELHKEVKE WVRKEIGAIA TPDVIHWAEG 610 620 630 640 LPKTRSGKIM RRILRKIATG ETDSLGDIST LADPGVVDKL IREKSEAA

References

[1]

"The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen." Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L. BMC Genomics 9:427-427(2008) [PubMed: 18801193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A449.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000644 Genomic DNA. Translation: ABO89098.1.
RefSeq	YP_001140846.1. NC_009348.1.
3D structure databases
ProteinModelPortal	A4SJM6.
SMR	A4SJM6. Positions 7-644.
ModBase	Search...
Protein-protein interaction databases
STRING	A4SJM6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4995560.
GenomeReviews	Gene locus ASA_0967 in contig CP000644_GR.
KEGG	asa:ASA_0967.
PATRIC	20788285. VBIAerSal2987_0961.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0365.
HOGENOM	HBG547964.
OMA	TRGTEES.
PhylomeDB	A4SJM6.
ProtClustDB	PRK00174.
Enzyme and pathway databases
BioCyc	ASAL382245:ASA_0967-MONOMER.
Family and domain databases
HAMAP	MF_01123. Ac_CoA_synth. [Tree]
InterPro	IPR011904. Ac_CoA_lig. IPR024597. Acyl-CoA_synth_DUF3448. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
KO	K01895.
PANTHER	PTHR24095:SF42. PTHR24095:SF42. 1 hit.
Pfam	PF00501. AMP-binding. 1 hit. PF11930. DUF3448. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA_AERS4

Accession

Primary (citable) accession number: A4SJM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 15, 2007
Last modified:	December 14, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program