A4SIX8 (ISPH_AERS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	ASA_0687

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000021081

Sequences

	Sequence		Length	Mass (Da)
	A4SIX8 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 3BCE319FA7C6B276	FASTA	311	34,111
	10 20 30 40 50 60 MDILLANPRG FCAGVDRAIS IVESALEKFG APIYVRHEVV HNRYVVNKLK EAGAVFVEEL 70 80 90 100 110 120 DEVPDDSIVI FSAHGVAKTV REMAKSRALK VFDATCPLVT KVHMEVHRAS RKGSEAVLIG 130 140 150 160 170 180 HAGHPEVIGT MGQYENREGG MYLVETPDDV ARLKVKNPDD LCFVTQTTLS VDETSDVIDA 190 200 210 220 230 240 LRKQFPKIQG PRKDDICYAT QNRQDAVREM AGLVDVMLVV GSRNSSNSNR LRELAEKVGS 250 260 270 280 290 300 KAYLIDDASM IETVWLEGVK AIGVTAGASA PDVLVQNVIA RLRELGGNMV VEHPGREENV 310 VFEVPPELRI I

References

[1]

"The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen." Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L. BMC Genomics 9:427-427(2008) [PubMed: 18801193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A449.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000644 Genomic DNA. Translation: ABO88850.1.
RefSeq	YP_001140598.1. NC_009348.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4SIX8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4996706.
GenomeReviews	Gene locus ASA_0687 in contig CP000644_GR.
KEGG	asa:ASA_0687.
PATRIC	20787704. VBIAerSal2987_0695.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0761.
HOGENOM	HBG335228.
OMA	VQEMQLL.
PhylomeDB	A4SIX8.
ProtClustDB	PRK01045.
Enzyme and pathway databases
BioCyc	ASAL382245:ASA_0687-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_AERS4

Accession

Primary (citable) accession number: A4SIX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 15, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program