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A4SIX5

- SYI_AERS4

UniProt

A4SIX5 - SYI_AERS4

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Aeromonas salmonicida (strain A449)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (15 May 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei577 – 5771Aminoacyl-adenylateUniRule annotation
    Binding sitei621 – 6211ATPUniRule annotation
    Metal bindingi916 – 9161ZincUniRule annotation
    Metal bindingi919 – 9191ZincUniRule annotation
    Metal bindingi936 – 9361ZincUniRule annotation
    Metal bindingi939 – 9391ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciASAL382245:GJJN-680-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:ASA_0684
    OrganismiAeromonas salmonicida (strain A449)
    Taxonomic identifieri382245 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
    ProteomesiUP000000225: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 953953Isoleucine--tRNA ligasePRO_1000022038Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi382245.ASA_0684.

    Structurei

    3D structure databases

    ProteinModelPortaliA4SIX5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi618 – 6225"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4SIX5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDYKHTLNL PETEFPMRGD LAKREPNMLK RWYDQDLYGA IRRAKAGKPS    50
    FILHDGPPYA NGSIHIGHSV NKILKDVIIK SKGLSGFDSP YVPGWDCHGL 100
    PIELKVEGMV GKPGEKVSAA EFRAECRKYA KTQIEAQKTD FIRLGVLGDW 150
    EHPYLTMDFG TEANIIRSMA KIVENGHLHK GSKPVHWCTD CGSALAEAEV 200
    EYYDKNSPSI DVRFKAVDEA TVAAKFDCPE GHLGKGPISA VIWTTTPWTL 250
    PANRAIAMHA DLDYALVQVE GEHPERLILA AELVKDVMDR AGIEKFHNLG 300
    YTKGVALELL RFNHPFYSFD VPVVLGDHVT LDAGTGAVHT APGHGQEDFV 350
    VGQKYGLEVA NPVGSNGVYL PDTELFAGQH VFKANAAVVE VLTERGALLH 400
    HMVFNHSYPH CWRHKTPIIF RATPQWFISM EQKGLRQRAL EEIERIEKDG 450
    ITQHGQSGWV PAWGKNRIQA MVENRPDWCI SRQRTWGVPI SLFVHKETQQ 500
    LHPESVRLMH EVAKRVEQSG IQAWWDLDKA ELLGSDADMY DKVPDTLDVW 550
    FDSGSTHSSV VDARPEFNGH SADLYLEGSD QHRGWFMSSL MIGVAMKDKA 600
    PYNQVLTHGF TVDGQGRKMS KSIGNVVSPQ DVMNKLGADI LRLWVASTDY 650
    TGEMTVSDEI LKRSADAYRR IRNTARFLLA NLNGFNPATD MVAAADMVVV 700
    DRWAVGRAKA AQAEIVTAFE EYNFHGVTQK LMQFCSIEMG SFYLDVIKDR 750
    QYTAKADSLA RRSCQTALYH IAQAMVRWMA PIMSFTADEI WALLPGERSE 800
    FVFTEEWYDG LFGLDAGEQM DDAFWAEILT VRGEVNKALE AARGEKRIGG 850
    SLQAELTLFA KPELAERLNA LADELRFVLL TSKAKVVTAD TAPEGSVATE 900
    RADLWLSVAQ SAAAKCDRCW HHVEDVGTIA GHEEICGRCA TNVEGDGETR 950
    QFA 953
    Length:953
    Mass (Da):106,242
    Last modified:May 15, 2007 - v1
    Checksum:iDAACBBF073200EFB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000644 Genomic DNA. Translation: ABO88847.1.
    RefSeqiYP_001140595.1. NC_009348.1.

    Genome annotation databases

    EnsemblBacteriaiABO88847; ABO88847; ASA_0684.
    GeneIDi4998575.
    KEGGiasa:ASA_0684.
    PATRICi20787698. VBIAerSal2987_0692.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000644 Genomic DNA. Translation: ABO88847.1 .
    RefSeqi YP_001140595.1. NC_009348.1.

    3D structure databases

    ProteinModelPortali A4SIX5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 382245.ASA_0684.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABO88847 ; ABO88847 ; ASA_0684 .
    GeneIDi 4998575.
    KEGGi asa:ASA_0684.
    PATRICi 20787698. VBIAerSal2987_0692.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci ASAL382245:GJJN-680-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen."
      Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L.
      BMC Genomics 9:427-427(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: A449.

    Entry informationi

    Entry nameiSYI_AERS4
    AccessioniPrimary (citable) accession number: A4SIX5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: May 15, 2007
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3