A4SIX5 (SYI_AERS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isoleucine--tRNA ligase
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS

Gene names

Name:	ileS
Ordered Locus Names:	ASA_0684

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	953 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP MF_02002
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02002
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_02002
Subunit structure	Monomer By similarity. HAMAP MF_02002
Subcellular location	Cytoplasm By similarity HAMAP MF_02002.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP MF_02002
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 953	953	Isoleucine--tRNA ligase HAMAP MF_02002		PRO_1000022038
Regions
	Motif	58 – 68	11	"HIGH" region HAMAP MF_02002
	Motif	618 – 622	5	"KMSKS" region HAMAP MF_02002
Sites
	Metal binding	916	1	Zinc By similarity
	Metal binding	919	1	Zinc By similarity
	Metal binding	936	1	Zinc By similarity
	Metal binding	939	1	Zinc By similarity
	Binding site	577	1	Aminoacyl-adenylate By similarity
	Binding site	621	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4SIX5 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: DAACBBF073200EFB	FASTA	953	106,242
	10 20 30 40 50 60 MSDYKHTLNL PETEFPMRGD LAKREPNMLK RWYDQDLYGA IRRAKAGKPS FILHDGPPYA 70 80 90 100 110 120 NGSIHIGHSV NKILKDVIIK SKGLSGFDSP YVPGWDCHGL PIELKVEGMV GKPGEKVSAA 130 140 150 160 170 180 EFRAECRKYA KTQIEAQKTD FIRLGVLGDW EHPYLTMDFG TEANIIRSMA KIVENGHLHK 190 200 210 220 230 240 GSKPVHWCTD CGSALAEAEV EYYDKNSPSI DVRFKAVDEA TVAAKFDCPE GHLGKGPISA 250 260 270 280 290 300 VIWTTTPWTL PANRAIAMHA DLDYALVQVE GEHPERLILA AELVKDVMDR AGIEKFHNLG 310 320 330 340 350 360 YTKGVALELL RFNHPFYSFD VPVVLGDHVT LDAGTGAVHT APGHGQEDFV VGQKYGLEVA 370 380 390 400 410 420 NPVGSNGVYL PDTELFAGQH VFKANAAVVE VLTERGALLH HMVFNHSYPH CWRHKTPIIF 430 440 450 460 470 480 RATPQWFISM EQKGLRQRAL EEIERIEKDG ITQHGQSGWV PAWGKNRIQA MVENRPDWCI 490 500 510 520 530 540 SRQRTWGVPI SLFVHKETQQ LHPESVRLMH EVAKRVEQSG IQAWWDLDKA ELLGSDADMY 550 560 570 580 590 600 DKVPDTLDVW FDSGSTHSSV VDARPEFNGH SADLYLEGSD QHRGWFMSSL MIGVAMKDKA 610 620 630 640 650 660 PYNQVLTHGF TVDGQGRKMS KSIGNVVSPQ DVMNKLGADI LRLWVASTDY TGEMTVSDEI 670 680 690 700 710 720 LKRSADAYRR IRNTARFLLA NLNGFNPATD MVAAADMVVV DRWAVGRAKA AQAEIVTAFE 730 740 750 760 770 780 EYNFHGVTQK LMQFCSIEMG SFYLDVIKDR QYTAKADSLA RRSCQTALYH IAQAMVRWMA 790 800 810 820 830 840 PIMSFTADEI WALLPGERSE FVFTEEWYDG LFGLDAGEQM DDAFWAEILT VRGEVNKALE 850 860 870 880 890 900 AARGEKRIGG SLQAELTLFA KPELAERLNA LADELRFVLL TSKAKVVTAD TAPEGSVATE 910 920 930 940 950 RADLWLSVAQ SAAAKCDRCW HHVEDVGTIA GHEEICGRCA TNVEGDGETR QFA

References

[1]

"The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen." Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L. BMC Genomics 9:427-427(2008) [PubMed: 18801193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A449.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000644 Genomic DNA. Translation: ABO88847.1.
RefSeq	YP_001140595.1. NC_009348.1.
3D structure databases
ProteinModelPortal	A4SIX5.
ModBase	Search...
Protein-protein interaction databases
STRING	A4SIX5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4998575.
GenomeReviews	Gene locus ASA_0684 in contig CP000644_GR.
KEGG	asa:ASA_0684.
PATRIC	20787698. VBIAerSal2987_0692.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0060.
HOGENOM	HBG577712.
OMA	KQVLTHG.
PhylomeDB	A4SIX5.
ProtClustDB	PRK05743.
Enzyme and pathway databases
BioCyc	ASAL382245:ASA_0684-MONOMER.
Family and domain databases
HAMAP	MF_02002. Ile_tRNA_synth_type1. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-synt. IPR023585. Ile-tRNA-synt_type1. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. IPR010663. Znf_DNA_glyclase/IsotRNA_synth. [Graphical view]
Gene3D	G3DSA:3.90.740.10. G3DSA:3.90.740.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01870.
PANTHER	PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF06827. zf-FPG_IleRS. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. IleS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_AERS4

Accession

Primary (citable) accession number: A4SIX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 15, 2007
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program