A4SIV0 (MDH_AERS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	ASA_0659

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas › Aeromonas salmonicida › Aeromonas salmonicida subsp. salmonicida

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_01516
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_01516
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Malate dehydrogenase HAMAP-Rule MF_01516		PRO_0000294293
Regions
	Nucleotide binding	7 – 13	7	NAD By similarity
	Nucleotide binding	117 – 119	3	NAD By similarity
Sites
	Active site	177	1	Proton acceptor By similarity
	Binding site	34	1	NAD By similarity
	Binding site	81	1	Substrate By similarity
	Binding site	87	1	Substrate By similarity
	Binding site	94	1	NAD By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	153	1	Substrate By similarity
	Binding site	227	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4SIV0 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: EC37D0AF9C8150B3	FASTA	311	32,139
	10 20 30 40 50 60 MKVAVLGAAG GIGQALALLL KNRLPAGSEL SLYDIAPVTP GVAVDLSHIP TDVKVKGFCG 70 80 90 100 110 120 EDPSPALVGA DVVLISAGVA RKPGMDRSDL FNINPGIVKN LVEKCAASCP KALIGIITNP 130 140 150 160 170 180 VNTTVAIAAE VLKKAGVYDK RRLFGVTTLD VIRAETFVAD AKGLNVDKVR VNVIGGHSGV 190 200 210 220 230 240 TILPLLSQIE GASFSAEEVA AMTKRIQNAG TEVVEAKAGG GSATLSMGQA ACRFGLSLIK 250 260 270 280 290 300 GLQGEANVIE CAYVEGDGKH ATFFAQPILL GKNGVETVLD YGKLSAFEQE AMEGMLATLK 310 ADIQLGVEFV K

References

[1]

"The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen." Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L. BMC Genomics 9:427-427(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A449.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000644 Genomic DNA. Translation: ABO88822.1.
RefSeq	YP_001140570.1. NC_009348.1.
3D structure databases
ProteinModelPortal	A4SIV0.
SMR	A4SIV0. Positions 1-310.
ModBase	Search...
Protein-protein interaction databases
STRING	382245.ASA_0659.
Proteomic databases
PRIDE	A4SIV0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABO88822; ABO88822; ASA_0659.
GeneID	4996474.
KEGG	asa:ASA_0659.
PATRIC	20787650. VBIAerSal2987_0668.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HOG000213792.
KO	K00024.
OMA	VEVKGFA.
ProtClustDB	PRK05086.
Enzyme and pathway databases
BioCyc	ASAL382245:GJJN-655-MONOMER.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_01516. Malate_dehydrog_1.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010097. Malate_DH_type1. IPR023958. Malate_DH_type1_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_AERS4

Accession

Primary (citable) accession number: A4SIV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	May 15, 2007
Last modified:	May 1, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program