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Protein

Argininosuccinate synthase

Gene

argG

Organism
Aeromonas salmonicida (strain A449)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei37 – 371ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei89 – 891CitrullineUniRule annotation
Binding sitei94 – 941CitrullineUniRule annotation
Binding sitei119 – 1191ATP; via amide nitrogenUniRule annotation
Binding sitei121 – 1211AspartateUniRule annotation
Binding sitei125 – 1251AspartateUniRule annotation
Binding sitei125 – 1251CitrullineUniRule annotation
Binding sitei126 – 1261AspartateUniRule annotation
Binding sitei129 – 1291CitrullineUniRule annotation
Binding sitei178 – 1781CitrullineUniRule annotation
Binding sitei187 – 1871CitrullineUniRule annotation
Binding sitei263 – 2631CitrullineUniRule annotation
Binding sitei275 – 2751CitrullineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciASAL382245:GJJN-577-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:ASA_0581
OrganismiAeromonas salmonicida (strain A449)
Taxonomic identifieri382245 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
Proteomesi
  • UP000000225 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 411411Argininosuccinate synthasePRO_0000321298Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi382245.ASA_0581.

Structurei

3D structure databases

ProteinModelPortaliA4SIM5.
SMRiA4SIM5. Positions 11-405.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CDH. Bacteria.
COG0137. LUCA.
HOGENOMiHOG000230093.
KOiK01940.
OMAiPAREWGM.
OrthoDBiEOG6K9QCV.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4SIM5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGINKIVLA YSGGLDTSAI IPWLKEHYDA EIIAFVADVG QERDDLEGIE
60 70 80 90 100
QKAIASGAVK CIVKDLREEF VKEYVYPTLK TGAVYEGTYL LGTSMARPII
110 120 130 140 150
AKAMVEAALA EGADAISHGC TGKGNDQVRF EGAVAALAPQ LKVIAPWRLW
160 170 180 190 200
DMRSREDLLA YLEARDIPCK ATLKKIYSRD ANAWHISTEG GELESTWNEP
210 220 230 240 250
SEAVWQWTVS AEQAPNEPEY VKLTVAKGEV VAVDDQPLSP HQILTTLNER
260 270 280 290 300
AGKHGVGRID ITENRMVGMK SRGCYETPGG TVMVAALRAV EELVLDRPTR
310 320 330 340 350
AWREKLGAEF SHLVYDGRWF TPLCKAIVAS ANAIAEDLDG EVILKMYKGQ
360 370 380 390 400
VTAVQKKSPN SLYSEDFATF GADEVYDQSH AEGFIRLYTL ASRIRAMKEQ
410
HQAIGGDHTH G
Length:411
Mass (Da):45,200
Last modified:April 13, 2016 - v2
Checksum:iEB11DDB0F8F7C8D8
GO

Sequence cautioni

The sequence ABO88747.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000644 Genomic DNA. Translation: ABO88747.1. Different initiation.

Genome annotation databases

EnsemblBacteriaiABO88747; ABO88747; ASA_0581.
KEGGiasa:ASA_0581.
PATRICi20787486. VBIAerSal2987_0586.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000644 Genomic DNA. Translation: ABO88747.1. Different initiation.

3D structure databases

ProteinModelPortaliA4SIM5.
SMRiA4SIM5. Positions 11-405.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi382245.ASA_0581.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO88747; ABO88747; ASA_0581.
KEGGiasa:ASA_0581.
PATRICi20787486. VBIAerSal2987_0586.

Phylogenomic databases

eggNOGiENOG4105CDH. Bacteria.
COG0137. LUCA.
HOGENOMiHOG000230093.
KOiK01940.
OMAiPAREWGM.
OrthoDBiEOG6K9QCV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciASAL382245:GJJN-577-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen."
    Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L.
    BMC Genomics 9:427-427(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: A449.

Entry informationi

Entry nameiASSY_AERS4
AccessioniPrimary (citable) accession number: A4SIM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: April 13, 2016
Last modified: April 13, 2016
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.