Reviewed, UniProtKB/Swiss-Prot A4SI57 (MURC_AERS4)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	ASA_0399

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_1000071093
Regions
	Nucleotide binding	126 – 132	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A4SI57-1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 90B1AC5377FCF2CC	FASTA	484	52,215
	10 20 30 40 50 60 MTKVELAKLR TMIPEMRRVR RIHFIGIGGA GMGGIAEVLA NEGYQVTGSD LARNAVTERL 70 80 90 100 110 120 ASLGAHIFVG HSAEHVSEAS VVVVSTAIKQ DNPELLAARE LRIPVVRRAE MLAELMRFRH 130 140 150 160 170 180 GIAIAGTHGK TTTTSLVASI YAEADRDPTF VIGGLLNSAG TNARLGSSRY LIAEADESDA 190 200 210 220 230 240 SFLHLQPMVS IVTNIEADHM ETYGGDFSKL KATFIDFLHN LPFYGLAVVC VDDPVIRGML 250 260 270 280 290 300 PEIARPTITY GLSDDADVQV LDFVQTSNRS CFRVRRKDAG ELAVTLNLPG IHNALNAAAA 310 320 330 340 350 360 IAVATEDGIG DDAIICALAK FEGVGRRFQQ YGEFETGRGK AMLVDDYGHH PSEVKVTINA 370 380 390 400 410 420 ARTGWPDKRL VMVFQPHRYT RTRDLYEDFS DVLSKVDVLV MLEVYAAGEE PIPGADGRAL 430 440 450 460 470 480 CRSIRSRGSL EPIFAATPDD VPAILADLIG EGDLVLTQGA GNVGALSRRL GEMKLSIDSM KSGA

References

[1]

"The genome sequence of Aeromonas salmonicida subsp. salmonicida A449." Reith M.E., Singh R.K., Curtis B., Boyd J., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J., Johnson S., Brown L. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000644 Genomic DNA. Translation: ABO88579.1.
RefSeq	YP_001140327.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4SI57.
Genome annotation databases
GeneID	4998411.
GenomeReviews	Gene locus ASA_0399 in contig CP000644_GR.
KEGG	asa:ASA_0399.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	EWMVVEA.
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_AERS4

Accession

Primary (citable) accession number: A4SI57

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	May 15, 2007
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)