A4SHR6 (ASNA_AERS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase
EC=6.3.1.1

Alternative name(s):
Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	ASA_0247

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000017933

Sequences

	Sequence		Length	Mass (Da)
	A4SHR6 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 3E5ADCE75199F7BB	FASTA	330	36,949
	10 20 30 40 50 60 MKQHYIRSQQ QISFVKEMFS RQLAQQLGLM EVQAPILSRV GDGTQDNLSG SENAVQVKVK 70 80 90 100 110 120 TLPGHSYEVV HSLAKWKRQT LGRFGFGPGE GIYTHMKALR PDEDKLTPIH SVYVDQWDWE 130 140 150 160 170 180 KVMPSERRDL AYLQETVRGI WAAIKATERA VCAEHELTPF LPGEIQFLHS EALLARYPDL 190 200 210 220 230 240 DAKGRERAIA KELGAVFLIG IGGALSHGER HDVRAPDYDD WSSHSELGLA GLNGDILVWN 250 260 270 280 290 300 PVLEDSFEIS SMGIRVDAEA LRRQLAITGD EGRLQYDWHR DLLAERMPQT IGGGIGQSRL 310 320 330 AMLLLQKEHI GQVQVGVWPS EMKAAIPGML

References

[1]

"The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen." Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L. BMC Genomics 9:427-427(2008) [PubMed: 18801193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A449.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000644 Genomic DNA. Translation: ABO88438.1.
RefSeq	YP_001140186.1. NC_009348.1.
3D structure databases
ProteinModelPortal	A4SHR6.
SMR	A4SHR6. Positions 5-330.
ModBase	Search...
Protein-protein interaction databases
STRING	A4SHR6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4996636.
GenomeReviews	Gene locus ASA_0247 in contig CP000644_GR.
KEGG	asa:ASA_0247.
PATRIC	20786790. VBIAerSal2987_0257.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2502.
HOGENOM	HBG288146.
OMA	LNDNLNG.
PhylomeDB	A4SHR6.
ProtClustDB	PRK05425.
Enzyme and pathway databases
BioCyc	ASAL382245:ASA_0247-MONOMER.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_AERS4

Accession

Primary (citable) accession number: A4SHR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 15, 2007
Last modified:	December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program