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A4SHL4

- CYSG1_AERS4

UniProt

A4SHL4 - CYSG1_AERS4

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Protein

Siroheme synthase 1

Gene
cysG1, ASA_0192
Organism
Aeromonas salmonicida (strain A449)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.UniRule annotation
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.UniRule annotation
Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotation
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei225 – 2251S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Active sitei248 – 2481Proton acceptor By similarity
Active sitei270 – 2701Proton donor By similarity
Binding sitei306 – 3061S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei383 – 3831S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding sitei412 – 4121S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 232NAD By similarity
Nucleotide bindingi43 – 442NAD By similarity

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. precorrin-2 dehydrogenase activity Source: UniProtKB-HAMAP
  3. sirohydrochlorin ferrochelatase activity Source: UniProtKB-EC
  4. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-HAMAP
  2. siroheme biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

NAD, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciASAL382245:GJJN-190-MONOMER.
UniPathwayiUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme synthase 1
Including the following 3 domains:
Uroporphyrinogen-III C-methyltransferase 1 (EC:2.1.1.107)
Short name:
Urogen III methylase 1
Alternative name(s):
SUMT 1
Uroporphyrinogen III methylase 1
Short name:
UROM 1
Precorrin-2 dehydrogenase 1 (EC:1.3.1.76)
Sirohydrochlorin ferrochelatase 1 (EC:4.99.1.4)
Gene namesi
Name:cysG1
Ordered Locus Names:ASA_0192
OrganismiAeromonas salmonicida (strain A449)
Taxonomic identifieri382245 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
ProteomesiUP000000225: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 468468Siroheme synthase 1UniRule annotationPRO_0000330489Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi382245.ASA_0192.

Structurei

3D structure databases

ProteinModelPortaliA4SHL4.
SMRiA4SHL4. Positions 1-460.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 204204precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase By similarityAdd
BLAST
Regioni216 – 468253Uroporphyrinogen-III C-methyltransferase By similarityAdd
BLAST
Regioni301 – 3033S-adenosyl-L-methionine binding By similarity
Regioni331 – 3322S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

In the C-terminal section; belongs to the precorrin methyltransferase family.

Phylogenomic databases

eggNOGiCOG0007.
HOGENOMiHOG000290518.
KOiK02302.
OMAiRAMATRD.
OrthoDBiEOG6DRPFR.

Family and domain databases

Gene3Di1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01646. Siroheme_synth.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4SHL4-1 [UniParc]FASTAAdd to Basket

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MDYLPIFCRL DNKPVLLVGG GEVAERKARL LLDAGARLTV VSPALDPELA    50
ALATSGTIDW LAAEFEPAHL TGKWLVVAAT DRREVNALVY QSANQAGIFA 100
NVVDDPKRSS FIMPSIIDRS PLMVAISSGG KAPVLARLLR EKLEALLPQH 150
LGAVAAFAGG LRARVKARFA SMGERRQFWE RLLSADRLGQ ALARGDKASA 200
NQLADTLFAE ETGAKGEVIL VGAGPGDPGL LTLHALRHMQ QADLVVYDQL 250
VSDEVMALVR RDARRIFVGK QAGNHCVPQE GINQLLLEEA SKGQRVVRLK 300
GGDPFIFGRG GEELETLVGS GVGFQVVPGI TAASGCAAYA GIPLTHRDHA 350
QSVRFVTAHG KGGTQDLDWP LLARDQQTLV FYMGLSSCAT IRQKLTAHGK 400
AGTTPVALIE RGTQLNQRVI RGTLDQLPEL AVGVESPALI MVGSVVTLAD 450
KLAWFGQTNH GVQAAALA 468
Length:468
Mass (Da):49,834
Last modified:May 15, 2007 - v1
Checksum:i41162FD6A3A0A080
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000644 Genomic DNA. Translation: ABO88386.1.
RefSeqiYP_001140134.1. NC_009348.1.

Genome annotation databases

EnsemblBacteriaiABO88386; ABO88386; ASA_0192.
GeneIDi4998078.
KEGGiasa:ASA_0192.
PATRICi20786673. VBIAerSal2987_0200.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000644 Genomic DNA. Translation: ABO88386.1 .
RefSeqi YP_001140134.1. NC_009348.1.

3D structure databases

ProteinModelPortali A4SHL4.
SMRi A4SHL4. Positions 1-460.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 382245.ASA_0192.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABO88386 ; ABO88386 ; ASA_0192 .
GeneIDi 4998078.
KEGGi asa:ASA_0192.
PATRICi 20786673. VBIAerSal2987_0200.

Phylogenomic databases

eggNOGi COG0007.
HOGENOMi HOG000290518.
KOi K02302.
OMAi RAMATRD.
OrthoDBi EOG6DRPFR.

Enzyme and pathway databases

UniPathwayi UPA00148 ; UER00211 .
UPA00148 ; UER00222 .
UPA00262 ; UER00211 .
UPA00262 ; UER00222 .
UPA00262 ; UER00376 .
BioCyci ASAL382245:GJJN-190-MONOMER.

Family and domain databases

Gene3Di 1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPi MF_01646. Siroheme_synth.
InterProi IPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view ]
Pfami PF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMi SSF53790. SSF53790. 1 hit.
TIGRFAMsi TIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEi PS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen."
    Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L.
    BMC Genomics 9:427-427(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: A449.

Entry informationi

Entry nameiCYSG1_AERS4
AccessioniPrimary (citable) accession number: A4SHL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 15, 2007
Last modified: June 11, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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