A4SHE9 (ILVD_AERS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	ASA_0122

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	613 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 613	613	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000000953
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A4SHE9 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 8854AB013FA182B6	FASTA	613	65,115
	10 20 30 40 50 60 MPKLRSATTT HGRNMAGARA LWRATGMTDE DFGKPIIAVV NSFTQFVPGH VHLKDLGQLV 70 80 90 100 110 120 AREIEAAGGV AKEFNTIAVD DGIAMGHGGM LYSLPSRELI ADSVEYMVNA HCADAMVCIS 130 140 150 160 170 180 NCDKITPGML MAALRINIPV IFVSGGPMEA GKTKLSDQII KLDLVDAMIQ GADPKVSDAQ 190 200 210 220 230 240 SEQVERSACP TCGSCSGMFT ANSMNCLTEA LGLSQPGNGS MLATHADREQ LFKLAGQRIV 250 260 270 280 290 300 TLAKRYYQQD DESALPRNIA TKAAFENAMA LDIAMGGSTN TVLHLLAAAQ EAGVDFTMAD 310 320 330 340 350 360 IDRMSRKVPQ LCKVAPSTQK YHMEDVHRAG GVVAILGQLE KAGLVHGDTR TVLGGSLAEL 370 380 390 400 410 420 LNEYDVSRQP SQEVVDFYRA GPAGIRTTKA FSQDCRWPEL DVDRAEGCIR SLEHAYSLEG 430 440 450 460 470 480 GLAVLSGNLA LDGAIVKTAG VDDEHLCFRG PARVFESQDT AVAGILDGTV KAGEVVVIRY 490 500 510 520 530 540 EGPKGGPGMQ EMLYPTTYLK SMGLGKQCAL ITDGRFSGGT SGLSIGHVSP EAASGGTIGL 550 560 570 580 590 600 VADGDIININ IPARSMVLEV ADSVLAARRV AVEARGWKPL DRQRQVSFAL RAYAMLATSA 610 DKGAVRDRSK LEE

References

[1]

"The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen." Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L. BMC Genomics 9:427-427(2008) [PubMed: 18801193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A449.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000644 Genomic DNA. Translation: ABO88321.1.
RefSeq	YP_001140069.1. NC_009348.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4SHE9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4994987.
GenomeReviews	Gene locus ASA_0122 in contig CP000644_GR.
KEGG	asa:ASA_0122.
PATRIC	20786536. VBIAerSal2987_0133.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	SQDCRWP.
PhylomeDB	A4SHE9.
ProtClustDB	PRK12448.
Enzyme and pathway databases
BioCyc	ASAL382245:ASA_0122-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_AERS4

Accession

Primary (citable) accession number: A4SHE9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 15, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program