A4SH46 (DNAA_AERS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromosomal replication initiator protein DnaA

Gene names

Name:	dnaA
Ordered Locus Names:	ASA_0001

Organism

Aeromonas salmonicida (strain A449) [Complete proteome] [HAMAP]

Taxonomic identifier

382245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids By similarity. HAMAP MF_00377
Subcellular location	Cytoplasm By similarity HAMAP MF_00377.
Sequence similarities	Belongs to the DnaA family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA-dependent DNA replication initiation Inferred from electronic annotation. Source: InterPro regulation of DNA replication Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA replication origin binding Inferred from electronic annotation. Source: InterPro nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 456	456	Chromosomal replication initiator protein DnaA HAMAP MF_00377		PRO_1000048600
Regions
	Nucleotide binding	161 – 168	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A4SH46 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 30D3026CC40162EA	FASTA	456	51,861
	10 20 30 40 50 60 MTASLWQQCL NRLQDELPSA EFSMWIRPLQ AELSDNTLTL YAPNRFVLDW VRDKYLIRVN 70 80 90 100 110 120 GIINELCGVD GPTLRFDIGN RPHPVAIARA PARGAAPVNN LQKSWESKAD AKPEPNHKSN 130 140 150 160 170 180 TNVNYTFENF VEGKSNQLAR AAARQVADNP GGAYNPLFLY GGTGLGKTHL LHAVGNAIKE 190 200 210 220 230 240 RKKDAKVIYM HSERFVQDMV KALQNNAIEE FKRYYRSVDA LLIDDIQFFA NKERSQEEFF 250 260 270 280 290 300 HTFNALLEGN QQIILTSDRY PKEINGVEDR LKSRFGWGLT VAIEPPELET RVAILMRKAD 310 320 330 340 350 360 ENQIHLPDEV AFFIAKRLRS NVRELEGALN RVIANANFTG RAINIDFVRE ALRDLLALQE 370 380 390 400 410 420 KLVTIDNIQK TVAEYYKIKL ADLLSKRRSR SVARPRQLAM ALAKELTNHS LPEIGDAFGG 430 440 450 REHTTVLHAC RKIEQLKEES HDIKEDYSNL IRTLSS

References

[1]

"The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen." Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C., Brown L.L. BMC Genomics 9:427-427(2008) [PubMed: 18801193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A449.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000644 Genomic DNA. Translation: ABO88218.1.
RefSeq	YP_001139966.1. NC_009348.1.
3D structure databases
ProteinModelPortal	A4SH46.
SMR	A4SH46. Positions 4-82, 363-456.
ModBase	Search...
Protein-protein interaction databases
STRING	A4SH46.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4998344.
GenomeReviews	Gene locus ASA_0001 in contig CP000644_GR.
KEGG	asa:ASA_0001.
PATRIC	20786282. VBIAerSal2987_0015.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0593.
HOGENOM	HBG695666.
OMA	DEVAFLI.
PhylomeDB	A4SH46.
ProtClustDB	PRK00149.
Enzyme and pathway databases
BioCyc	ASAL382245:ASA_0001-MONOMER.
Family and domain databases
HAMAP	MF_00377. DnaA_bact. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR001957. Chromosome_initiator_DnaA. IPR020591. Chromosome_initiator_DnaA-like. IPR018312. Chromosome_initiator_DnaA_CS. IPR013159. DnaA_C. IPR013317. DnaA_Hda. IPR010921. Trp_repressor/repl_initiator. [Graphical view]
Gene3D	G3DSA:1.10.1750.10. DnaA_C. 1 hit.
KO	K02313.
Pfam	PF00308. Bac_DnaA. 1 hit. PF08299. Bac_DnaA_C. 1 hit. [Graphical view]
PRINTS	PR00051. DNAA.
SMART	SM00382. AAA. 1 hit. SM00760. Bac_DnaA_C. 1 hit. [Graphical view]
SUPFAM	SSF48295. Trp_repress_rep. 1 hit.
TIGRFAMs	TIGR00362. DnaA. 1 hit.
PROSITE	PS01008. DNAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAA_AERS4

Accession

Primary (citable) accession number: A4SH46

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 15, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program