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A4SGW5 (A4SGW5_PROVI) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Protein attributes

Sequence length803 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733

Sequence similarities

Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206

Contains 1 Toprim domain. SAAS SAAS023406

Sequences

Sequence LengthMass (Da)Tools
A4SGW5 [UniParc].

Last modified May 15, 2007. Version 1.
Checksum: F08D8C25DEAA169B

FASTA80389,767
        10         20         30         40         50         60 
MAVKTPSLSA KNRTLIVVES PSKAKTINKY LGDGYAVFAS VGHIKDLPKK EIGLDFDNHY 

        70         80         90        100        110        120 
EPSYEVIAGK DKVVRQLKKL SAEASAVMIA TDPDREGEAI AWHIANEIGK TSKPVTRVMF 

       130        140        150        160        170        180 
NEITKSAILE AIADPRQIDY KLVRSQQTRQ GLDKIVGYRI SPFLWNVVMR GLSAGRVQSV 

       190        200        210        220        230        240 
ALRLICEREM EINRFEIQEY WTIAADFITS EKESFKARLI SINGEKPDLK NQASAEAAAA 

       250        260        270        280        290        300 
LVSKGNYRLK AIAPRTQQRR APLPFTTSLL QQAASNQLGF GSKKTMRAAQ QLYEGIDLGS 

       310        320        330        340        350        360 
EGATGLITYM RTDSIRIGDE AKGQARHFIE NTFGKEYIGF GGAAAKSSKN AQDAHEAIRP 

       370        380        390        400        410        420 
TSVNRRPEDL KPYLSTDQYR LYDLIWKRFV AAMMAPAKIE QTRVDIADPE ARISFRANGS 

       430        440        450        460        470        480 
RVLFPGFMKV FNDQEELAYE ERKSTKDEGE KEQTVRLPKV LRENDPLNLG PIDSRQSFTR 

       490        500        510        520        530        540 
PPARFSEATL VKDLDNYGIG RPSTYASIFS TLQDRRYVEL QKKKIIPTVL GMDVSEILVS 

       550        560        570        580        590        600 
NFPELFNVDF TAGMENELDK VAVGEDDYES VLDRFYKPLE ATLSARKEDP VIPQNRDAAP 

       610        620        630        640        650        660 
CEKCGEGRMI VKWTQSGKFL GCSRYPKCRN NIPIATNKEK PKDTGILCPS CNEGHMLLRN 

       670        680        690        700        710        720 
GRHGAFLACS SYPKCKGLLN LGKQRQIEPM KLPPVLTDLT CPKCGAPMNL RIGKRGPWLG 

       730        740        750        760        770        780 
CSKFPKCRGR MAWNSLEEPV RLHWEEVMAE HIKANPAITI TMIDGNPASM TMPIDDIITS 

       790        800 
AEERGLVRIE AEEQAAEKTP VTE

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References

[1]"Complete sequence of Prosthecochloris vibrioformis DSM 265."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Mikhailova N., Li T., Overmann J., Schuster S.C., Bryant D.A., Richardson P.
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 265.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000607 Genomic DNA. Translation: ABP37724.1.
RefSeqYP_001131226.1. NC_009337.1.

3D structure databases

ProteinModelPortalA4SGW5.
ModBaseSearch...

Protein-protein interaction databases

STRINGA4SGW5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4971211.
GenomeReviewsGene locus Cvib_1714 in contig CP000607_GR.
KEGGpvi:Cvib_1714.
PATRIC21397970. VBIChlPha132153_1802.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0550.
HOGENOMHBG512935.
OMAEEYWSID.
PhylomeDBA4SGW5.
ProtClustDBPRK06599.

Family and domain databases

InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KOK03168.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 3 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. TopA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA4SGW5_PROVI
AccessionPrimary (citable) accession number: A4SGW5
Entry history
Integrated into UniProtKB/TrEMBL: May 15, 2007
Last sequence update: May 15, 2007
Last modified: December 14, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info