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Protein

Biotin synthase

Gene

bioB

Organism
Chlorobium phaeovibrioides (strain DSM 265 / 1930) (Prosthecochloris vibrioformis (strain DSM 265))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi71Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi75Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi78Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi150Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi211Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi281Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCPHA290318:G1G8E-1794-MONOMER
UniPathwayiUPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:Cvib_1711
OrganismiChlorobium phaeovibrioides (strain DSM 265 / 1930) (Prosthecochloris vibrioformis (strain DSM 265))
Taxonomic identifieri290318 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupChlorobium
Proteomesi
  • UP000006700 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003815491 – 332Biotin synthaseAdd BLAST332

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi290318.Cvib_1711

Structurei

3D structure databases

ProteinModelPortaliA4SGW2
SMRiA4SGW2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107SFF Bacteria
COG0502 LUCA
HOGENOMiHOG000239958
KOiK01012
OrthoDBiPOG091H01DF

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

A4SGW2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIKTLHPAI AAAYRVLETG EPISLEEAEL LASLPGEFSL DLASLANKVR
60 70 80 90 100
NRWGKGGIHA CSIMNAKSGV CGENCRFCAQ SRHNHADIEV YPLVDEDAVL
110 120 130 140 150
FEARSCAEHG VSHFGLVTSG YGYRTMNAEF KRILAMIDRL HEELPELNVC
160 170 180 190 200
ASIGILGPET AAALARHGIA HYNINLQVAP EKYAGLIADT HGIEERMETV
210 220 230 240 250
RLLRREGVNV CCGGIIGVGE SMEDRVAMLF ALRDLDVSVI PINVLVPIEG
260 270 280 290 300
TPLQAAESVP LADIVKVFAL ARLVHPHSII KFAAGRETLM KDFQGLLMLS
310 320 330
GADGYLTGGY LTTRGRDLAD DQRFSERLAS FS
Length:332
Mass (Da):36,051
Last modified:July 28, 2009 - v2
Checksum:iD0B4A9A1167A5001
GO

Sequence cautioni

The sequence ABP37721 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000607 Genomic DNA Translation: ABP37721.1 Different initiation.
RefSeqiWP_041470238.1, NC_009337.1

Genome annotation databases

EnsemblBacteriaiABP37721; ABP37721; Cvib_1711
KEGGipvi:Cvib_1711

Similar proteinsi

Entry informationi

Entry nameiBIOB_CHLPM
AccessioniPrimary (citable) accession number: A4SGW2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: April 25, 2018
This is version 68 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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