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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Chlorobium phaeovibrioides (strain DSM 265 / 1930) (Prosthecochloris vibrioformis (strain DSM 265))
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei46ATP; via amide nitrogenUniRule annotation1
Metal bindingi138Magnesium; catalyticUniRule annotation1
Active sitei163Proton acceptorUniRule annotation1
Binding sitei190Allosteric activator ADPUniRule annotation1
Binding sitei198Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei258SubstrateUniRule annotation1
Binding sitei282Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi107 – 108ATPUniRule annotation2
Nucleotide bindingi137 – 140ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationSAAS annotationImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:Cvib_1665Imported
OrganismiChlorobium phaeovibrioides (strain DSM 265 / 1930) (Prosthecochloris vibrioformis (strain DSM 265))Imported
Taxonomic identifieri290318 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupChlorobium
Proteomesi
  • UP000006700 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi290318.Cvib_1665.

Structurei

3D structure databases

ProteinModelPortaliA4SGR6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 314PFKInterPro annotationAdd BLAST277

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni56 – 60Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation5
Regioni161 – 163Substrate bindingUniRule annotation3
Regioni205 – 207Substrate bindingUniRule annotation3
Regioni221 – 223Allosteric activator ADP bindingUniRule annotation3
Regioni288 – 291Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CTQ. Bacteria.
COG0205. LUCA.
HOGENOMiHOG000248870.
KOiK00850.
OMAiVMGHEAG.
OrthoDBiPOG091H01AC.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP_prok.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4SGR6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGQPRLRES RTLHPPMATM TRKSDNDQLS FTSGRIKKIG ILTSGGDAPG
60 70 80 90 100
MNAAIRAATR SAIAAGREAV GILRGYQGMI DGDFIPLQST DVSGILQLGG
110 120 130 140 150
TMLKTARCKE FRTPEGRRRA KEQLTAAAID AVIVIGGDGS FTGAWVMSQE
160 170 180 190 200
CDIPFVGIPA TIDNDMYGTD YTIGYETALN SVVCAVDKIK DTARSHGRIF
210 220 230 240 250
FIEVMGHEAG LLALNSGIAC GAEVILIPES KAQHEELQKF LEKGYRDKES
260 270 280 290 300
SGIVMVAEGD EAGGALHIAE QIRRDHPDLD VRISILGHIQ RGGSPVARDR
310 320 330 340 350
INATRMGIAA VDALLNDQKS IMIGLDNNRI VHVTFNKAVK MNHSIDTGLL

EVHNLLNGM
Length:359
Mass (Da):38,658
Last modified:May 15, 2007 - v1
Checksum:i9F7F93EA3913ECE8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000607 Genomic DNA. Translation: ABP37675.1.

Genome annotation databases

EnsemblBacteriaiABP37675; ABP37675; Cvib_1665.
KEGGipvi:Cvib_1665.
PATRICi21397866. VBIChlPha132153_1750.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000607 Genomic DNA. Translation: ABP37675.1.

3D structure databases

ProteinModelPortaliA4SGR6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290318.Cvib_1665.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP37675; ABP37675; Cvib_1665.
KEGGipvi:Cvib_1665.
PATRICi21397866. VBIChlPha132153_1750.

Phylogenomic databases

eggNOGiENOG4105CTQ. Bacteria.
COG0205. LUCA.
HOGENOMiHOG000248870.
KOiK00850.
OMAiVMGHEAG.
OrthoDBiPOG091H01AC.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP_prok.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiA4SGR6_CHLPM
AccessioniPrimary (citable) accession number: A4SGR6
Entry historyi
Integrated into UniProtKB/TrEMBL: May 15, 2007
Last sequence update: May 15, 2007
Last modified: November 30, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.