A4SGL4 (A4SGL4_PROVI) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase HAMAP-Rule MF_00159
EC=1.17.7.1 HAMAP-Rule MF_00159

Alternative name(s):
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase HAMAP-Rule MF_00159

Gene names

Name:	ispG HAMAP-Rule MF_00159
Ordered Locus Names:	Cvib_1613 EMBL ABP37623.1

Organism

Prosthecochloris vibrioformis (strain DSM 265) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 265)) (Chlorobium phaeovibrioides (strain DSM 265)) [Complete proteome] [HAMAP] EMBL ABP37623.1

Taxonomic identifier

290318 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Bacteroidetes/Chlorobi group › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium › Chlorobium phaeovibrioides

Protein attributes

Sequence length	686 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate By similarity. HAMAP-Rule MF_00159 SAAS SAAS004588
Catalytic activity	(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin. HAMAP-Rule MF_00159 SAAS SAAS004588
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_00159 SAAS SAAS004588
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 5/6. HAMAP-Rule MF_00159 SAAS SAAS004588
Sequence similarities	Belongs to the IspG family. HAMAP-Rule MF_00159

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP-Rule MF_00159 SAAS SAAS004588
Ligand	4Fe-4S HAMAP-Rule MF_00159 SAAS SAAS004588 Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase HAMAP-Rule MF_00159 SAAS SAAS004588 EMBL ABP37623.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: UniProtKB-UniPathway terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	591	1	Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_00159
	Metal binding	594	1	Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_00159
	Metal binding	625	1	Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_00159
	Metal binding	632	1	Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_00159

Sequences

	Sequence		Length	Mass (Da)
	A4SGL4 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: D83406578372CAF2	FASTA	686	74,902
	10 20 30 40 50 60 MYQYRRRLTR EVSFGPISLG GYQPIRVESM TNTHTADVDA TVAQCRRLRD AGCEIIRLTV 70 80 90 100 110 120 PTEKDADSLR LIRDRLRGEG IDTPLVADIH FSARAAMKAV EFVENIRINP GNYASRPKRS 130 140 150 160 170 180 PGEDYTDEEY RAELEYVRGE FLPLVEKARR LGVSMRIGTN HGSLSDRIVS RRGNSPDGMV 190 200 210 220 230 240 EAALEFARMA EEAGYHDILF SMKSSNVRVM IQAYRLLVER ADSELRYAYP LHLGVTEAGD 250 260 270 280 290 300 GDEGRIKSAM GIGALLEDGL GDTIRVSLTE DPVNEVPVGF AIVKKYNDFY LIKGERAHLP 310 320 330 340 350 360 LKHLLEADWK GKNSGTDALF APFSPFSYAR RLSSPVELAG IPVGGDAPPA VETALHVPLS 370 380 390 400 410 420 DLEGATAEAL QRLRPDLGEQ AIRSEFVSIP VGSREDLQAF DALLARLGDA AGRVNASTAD 430 440 450 460 470 480 TGVFTELLKR EVAKVRLDIA EEELLPKELV SSLALSGRPA VEFSFIHNAA GIAAPAAVLG 490 500 510 520 530 540 MLAQKARSLS VDRVFLSVLS DTGLFATRKL VSECLRLGIS LPVVVRFRST ERDRLSVLIQ 550 560 570 580 590 600 SAVQAGSLFS DGIGDMISLE SVLEPADEIN LALNILQGAR IRMSKTEFIS CPGCGRTYFD 610 620 630 640 650 660 LEKAAASIKG RMAHLKGLKI GIMGCIVNGP GEMADADFGY VGAGKNRISL YVGKECVEEN 670 680 LPEDEAVERL IGLIRERGRW VDKVEG

References

[1]

"Complete sequence of Prosthecochloris vibrioformis DSM 265." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Mikhailova N., Li T., Overmann J., Schuster S.C., Bryant D.A., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 265 EMBL ABP37623.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000607 Genomic DNA. Translation: ABP37623.1.
RefSeq	YP_001131125.1. NC_009337.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	290318.Cvib_1613.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABP37623; ABP37623; Cvib_1613.
GeneID	4971096.
KEGG	pvi:Cvib_1613.
PATRIC	21397756. VBIChlPha132153_1696.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0821.
HOGENOM	HOG000261018.
KO	K03526.
OMA	SMRIGTN.
ProtClustDB	CLSK637188.
Enzyme and pathway databases
BioCyc	CPHA290318:GHNQ-1663-MONOMER.
UniPathway	UPA00056; UER00096.
Family and domain databases
HAMAP	MF_00159. IspG.
InterPro	IPR017178. IspG_atypical. IPR004588. IspG_bac-typ. [Graphical view]
Pfam	PF04551. GcpE. 2 hits. [Graphical view]
PIRSF	PIRSF037336. IspG_like. 1 hit.
TIGRFAMs	TIGR00612. ispG_gcpE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A4SGL4_PROVI

Accession

Primary (citable) accession number: A4SGL4

Entry history

Integrated into UniProtKB/TrEMBL:	May 15, 2007
Last sequence update:	May 15, 2007
Last modified:	May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)