A4SGC0 (ISPH_PROVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	Cvib_1518

Organism

Prosthecochloris vibrioformis (strain DSM 265) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 265)) (Chlorobium phaeovibrioides (strain DSM 265)) [Complete proteome] [HAMAP]

Taxonomic identifier

290318 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000077523

Sequences

	Sequence		Length	Mass (Da)
	A4SGC0 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: D3FF21F0B929B880	FASTA	313	34,900
	10 20 30 40 50 60 MKVNLDRNSS GFCFGVQSTI NVAEEKLRKE KGLYSLGDVV HNEVEVKRLE ALGLITIDTP 70 80 90 100 110 120 AFNELKNAPV LIRAHGEPPS TYETANRNNL TLTDTTCPVV AKLQRTASQL NELGYQIIIY 130 140 150 160 170 180 GKHLHPEVIG INGHCQNRAL IIKHADLSDP EETLPIDLSK KTALISQTTM DVSGFYELKK 190 200 210 220 230 240 NLEKLFSKGG RETGPWAEVK SIGEAEPMPE FIFKDTICRQ ISNRNQKLQD FSRANERIIF 250 260 270 280 290 300 VAGKKSSNGQ VLYAICKEAN PESHFIEDVE ELQSEWFTTT QGKTVESIGV CGATSTPMWL 310 LEKVANHIEA HYA

References

[1]

"Complete sequence of Prosthecochloris vibrioformis DSM 265." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Mikhailova N., Li T., Overmann J., Schuster S.C., Bryant D.A., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 265.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000607 Genomic DNA. Translation: ABP37529.1.
RefSeq	YP_001131031.1. NC_009337.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4SGC0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4970659.
GenomeReviews	Gene locus Cvib_1518 in contig CP000607_GR.
KEGG	pvi:Cvib_1518.
PATRIC	21397544. VBIChlPha132153_1597.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0761.
HOGENOM	HBG335228.
OMA	HNQGKRY.
ProtClustDB	PRK01045.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_PROVI

Accession

Primary (citable) accession number: A4SGC0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 15, 2007
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program