A4SGA5 (SYE_PROVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	Cvib_1503

Organism

Prosthecochloris vibrioformis (strain DSM 265) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 265)) (Chlorobium phaeovibrioides (strain DSM 265)) [Complete proteome] [HAMAP]

Taxonomic identifier

290318 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	521 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 521	521	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000367739
Regions
	Motif	30 – 40	11	"HIGH" region HAMAP MF_00022_B
	Motif	277 – 281	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	280	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4SGA5 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: AE751037843FB84D	FASTA	521	59,828
	10 20 30 40 50 60 MFLTDGSSLN SLTMTRRMMV GKRVRTRFAP SPTGYLHVGG LRTALYNYLF AKRMDGDFVV 70 80 90 100 110 120 RIEDTDQSRK VADAQENLIK TLEWAGLMPD ESPLHGGDFG PYLQSERLDI YKKYCEQLLE 130 140 150 160 170 180 AGHAYHCFAT SEELEENRQL QLKQGLQPKY NRKWLPEDMG GSMPRSEIQK KLDEGVPSVV 190 200 210 220 230 240 RMKVPDYVSV WFEDIIRGPI EFDSATIDDQ VLMKSDGFPT YHFASVIDDH LMEFTHIIRG 250 260 270 280 290 300 EEWLPSMPKH LLLYEFLGWE PPKYAHLPLL LNPDRSKLSK RQGDVSVEDY IRKGYSGEAI 310 320 330 340 350 360 VNFVALLGWN QGEGCEQEVY SLQELTERFS LERVGKAGSI FTIDKLNWLE KQYIKNRPAE 370 380 390 400 410 420 DIIRVIKPLL LSELEKKETL LDPATITGER YLEDVIELMR ERVGFEREFV TFSSYFFFEP 430 440 450 460 470 480 ETYEEDAVKK RWTPDTNSLL DEFLPVLESM PDFTAEAIEA ALKEFVAPKG LKAAALIHPL 490 500 510 520 RIVSSGVSFG PSLYHMLEVL GREAVVRRIR KGMAVITLPQ Q

References

[1]

"Complete sequence of Prosthecochloris vibrioformis DSM 265." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Mikhailova N., Li T., Overmann J., Schuster S.C., Bryant D.A., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 265.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000607 Genomic DNA. Translation: ABP37514.1.
RefSeq	YP_001131016.1. NC_009337.1.
3D structure databases
ProteinModelPortal	A4SGA5.
ModBase	Search...
Protein-protein interaction databases
STRING	A4SGA5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4970581.
GenomeReviews	Gene locus Cvib_1503 in contig CP000607_GR.
KEGG	pvi:Cvib_1503.
PATRIC	21397514. VBIChlPha132153_1582.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	MAHIPLI.
PhylomeDB	A4SGA5.
ProtClustDB	PRK01406.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_PROVI

Accession

Primary (citable) accession number: A4SGA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 15, 2007
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program