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A4SG27 (SYL_PROVI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Cvib_1425
OrganismProsthecochloris vibrioformis (strain DSM 265) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 265)) (Chlorobium phaeovibrioides (strain DSM 265)) [Complete proteome] [HAMAP]
Taxonomic identifier290318 [NCBI]
Taxonomic lineageBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupChlorobium

Protein attributes

Sequence length805 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 805805Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000074839

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif576 – 5805"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5791ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4SG27 [UniParc].

Last modified May 15, 2007. Version 1.
Checksum: EA7A3A78D650EE00

FASTA80591,792
        10         20         30         40         50         60 
MRYDFSKTEK KWQARWKQQE TFKTGEAPEK PKYYVLDMFP YPSGSGLHVG HLEGYTASDI 

        70         80         90        100        110        120 
SARYRRSRGY NVLHPMGWDA FGLPAEQFAI KTGTHPSDTT QKNIANFKET LQAMGFSYDW 

       130        140        150        160        170        180 
SREINTTDPG YFQWTQWIFL KLHEMGLAYM SEVDVNWCVE LRAVLANEEV EEKLADGLTV 

       190        200        210        220        230        240 
VRRPLRQWVL KITAYADRLL EDLDGLDWPE NVKQMQRNWI GRSEGVEVDF ELRCHRTMLR 

       250        260        270        280        290        300 
VYTTRPDTLF GATYLVISPE HAMAEKLATA QQLVAVKEYI AKAKLKTELE RTGLQKDKTG 

       310        320        330        340        350        360 
VFTGSYAINP ATGEPLPVWI SDFVLTSYGT GAIMSVPAHD SRDWEFAKQY GLPIIEVVKS 

       370        380        390        400        410        420 
PHDVQQEVFE GKDSTVVNSS NNDISLNGLA FPEAFELMAS WLEKKKAGKR KVNYRLRDWI 

       430        440        450        460        470        480 
FSRQRYWGEP IPIKHYPDGT LKPETSLPLM LPEVEAYQPT ETGESPLANI PEWLNGTDEN 

       490        500        510        520        530        540 
GPFRRETNTM PQWAGSCWYY LRFIDPHNSK LPVDPAKEAY WMNVDLYIGG AEHAVLHLLY 

       550        560        570        580        590        600 
ARFWHKVLFD LKVVSTKEPF QRLFNQGMIL GEDNEKMSKS RGNVIPADHV LQTYGADAVR 

       610        620        630        640        650        660 
LYEMFLGPLE QVKPWNTNGI EGISRFLSRV WRLVWPEPEG TRADLSTLAP SPDLLRRMHK 

       670        680        690        700        710        720 
TIKKVAADTE NLKFNTAIAE MMVFTNELHR TGEGSRIAVE TLLLLLAPYA PHLSEELWEA 

       730        740        750        760        770        780 
LGHQESISLA PFPEFDPELA QDKEVTIAVQ VNGKLRGSFT AAPGSPKEQL LNEAKALEGV 

       790        800 
KKFLEGVTIM KEIVVPDKLI NFAVK 

« Hide

References

[1]"Complete sequence of Prosthecochloris vibrioformis DSM 265."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Mikhailova N., Li T., Overmann J., Schuster S.C., Bryant D.A., Richardson P.
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 265.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000607 Genomic DNA. Translation: ABP37436.1.
RefSeqYP_001130938.1. NC_009337.1.

3D structure databases

ProteinModelPortalA4SG27.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290318.Cvib_1425.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP37436; ABP37436; Cvib_1425.
GeneID4971325.
KEGGpvi:Cvib_1425.
PATRIC21397350. VBIChlPha132153_1501.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycCPHA290318:GHNQ-1463-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL_PROVI
AccessionPrimary (citable) accession number: A4SG27
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 15, 2007
Last modified: May 14, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries