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A4SFZ0 (ISPF_PROVI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Short name=MECDP-synthase
Short name=MECPP-synthase
Short name=MECPS
EC=4.6.1.12
Gene names
Name:ispF
Ordered Locus Names:Cvib_1388
OrganismProsthecochloris vibrioformis (strain DSM 265) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 265)) (Chlorobium phaeovibrioides (strain DSM 265)) [Complete proteome] [HAMAP]
Taxonomic identifier290318 [NCBI]
Taxonomic lineageBacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium ›

Protein attributes

Sequence length157 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) By similarity. HAMAP-Rule MF_00107

Catalytic activity

2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. HAMAP-Rule MF_00107

Cofactor

Binds 1 divalent metal cation per subunit By similarity.

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6. HAMAP-Rule MF_00107

Subunit structure

Homotrimer By similarity.

Sequence similarities

Belongs to the IspF family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 1571572-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase HAMAP-Rule MF_00107
PRO_1000075917

Regions

Region8 – 103Substrate binding By similarity
Region34 – 352Substrate binding By similarity
Region38 – 469Substrate binding By similarity
Region56 – 583Substrate binding By similarity
Region131 – 1355Substrate binding By similarity

Sites

Metal binding81Divalent metal cation By similarity
Metal binding101Divalent metal cation By similarity
Metal binding421Divalent metal cation By similarity
Binding site1421Substrate By similarity
Site341Transition state stabilizer By similarity
Site1331Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
A4SFZ0 [UniParc].

Last modified May 15, 2007. Version 1.
Checksum: 4656655D304BD9AE

FASTA15716,829
        10         20         30         40         50         60 
MRIGIGIDVH SFKEGRKLIV GGVDIPSPKG LDGHSDADVL LHAVSDALLG AAALGDIGLH 

        70         80         90        100        110        120 
FPDTSPEFKD IDSMILLKHV GKLLEKNGWR TVNVDAMLLL EAPKIAPFVV DMRKNIARCL 

       130        140        150 
QIETDLVSVK ATTNEKLGYI GREEGAAAHA VCIIEKA

« Hide

References

[1]"Complete sequence of Prosthecochloris vibrioformis DSM 265."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Mikhailova N., Li T., Overmann J., Schuster S.C., Bryant D.A., Richardson P.
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 265.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000607 Genomic DNA. Translation: ABP37399.1.
RefSeqYP_001130901.1. NC_009337.1.

3D structure databases

ProteinModelPortalA4SFZ0.
SMRA4SFZ0. Positions 1-156.
ModBaseSearch...

Protein-protein interaction databases

STRING290318.Cvib_1388.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP37399; ABP37399; Cvib_1388.
GeneID4969973.
KEGGpvi:Cvib_1388.
PATRIC21397276. VBIChlPha132153_1464.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0245.
HOGENOMHOG000239175.
KOK01770.
OMAHKFGGEG.
ProtClustDBPRK00084.

Enzyme and pathway databases

BioCycCPHA290318:GHNQ-1438-MONOMER.
UniPathwayUPA00056; UER00095.

Family and domain databases

Gene3D3.30.1330.50. 1 hit.
HAMAPMF_00107. IspF.
InterProIPR003526. MECDP_synthase.
IPR020555. MECDP_synthase_CS.
[Graphical view]
PfamPF02542. YgbB. 1 hit.
[Graphical view]
SUPFAMSSF69765. YgbB_synth. 1 hit.
TIGRFAMsTIGR00151. ispF. 1 hit.
PROSITEPS01350. ISPF. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameISPF_PROVI
AccessionPrimary (citable) accession number: A4SFZ0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 15, 2007
Last modified: May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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