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Protein

NH(3)-dependent NAD(+) synthetase

Gene

nadE

Organism
Chlorobium phaeovibrioides (strain DSM 265 / 1930) (Prosthecochloris vibrioformis (strain DSM 265))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.UniRule annotation

Catalytic activityi

ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. NH(3)-dependent NAD(+) synthetase (nadE)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi42MagnesiumUniRule annotation1
Binding sitei118Deamido-NADUniRule annotation1
Binding sitei138ATPUniRule annotation1
Metal bindingi143MagnesiumUniRule annotation1
Binding sitei167ATPUniRule annotation1
Binding sitei189ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi36 – 43ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciCPHA290318:G1G8E-1274-MONOMER
UniPathwayiUPA00253; UER00333

Names & Taxonomyi

Protein namesi
Recommended name:
NH(3)-dependent NAD(+) synthetaseUniRule annotation (EC:6.3.1.5UniRule annotation)
Gene namesi
Name:nadEUniRule annotation
Ordered Locus Names:Cvib_1211
OrganismiChlorobium phaeovibrioides (strain DSM 265 / 1930) (Prosthecochloris vibrioformis (strain DSM 265))
Taxonomic identifieri290318 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupChlorobium
Proteomesi
  • UP000006700 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000775791 – 277NH(3)-dependent NAD(+) synthetaseAdd BLAST277

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi290318.Cvib_1211

Structurei

3D structure databases

ProteinModelPortaliA4SFG4
SMRiA4SFG4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4K Bacteria
COG0171 LUCA
HOGENOMiHOG000238069
KOiK01916
OMAiCAINPIG
OrthoDBiPOG091H00G3

Family and domain databases

CDDicd00553 NAD_synthase, 1 hit
Gene3Di3.40.50.620, 1 hit
HAMAPiMF_00193 NadE_ammonia_dep, 1 hit
InterProiView protein in InterPro
IPR022310 NAD/GMP_synthase
IPR003694 NAD_synthase
IPR022926 NH(3)-dep_NAD(+)_synth
IPR014729 Rossmann-like_a/b/a_fold
PANTHERiPTHR23090 PTHR23090, 1 hit
PfamiView protein in Pfam
PF02540 NAD_synthase, 1 hit
TIGRFAMsiTIGR00552 nadE, 1 hit

Sequencei

Sequence statusi: Complete.

A4SFG4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVQDLDLNY QLVEEILSSF LRNEIRKFGF QSLVLGLSGG IDSAVVCELA
60 70 80 90 100
VRALGAENVL AVKMPYRASS RESLEHAELM VERLSIRSEE HDISQPVDAF
110 120 130 140 150
FTGIPEESRL RRGNIMARAR MIVLYDVSAR DGCLVAGTSN KTELLLGYGT
160 170 180 190 200
MFGDMASAVN PIGDLYKSQV RGLARHLGIP AALIDKAPSA DLWQGQSDEA
210 220 230 240 250
DLGFTYEEVD ILLYQMLELR MDKESILAEG VPEPFYARVR QMVVRNQYKR
260 270
LMPVIAKISG RTPGIDFRYA RDWQEIS
Length:277
Mass (Da):31,039
Last modified:May 15, 2007 - v1
Checksum:i2C6C51DF3490E784
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000607 Genomic DNA Translation: ABP37223.1
RefSeqiWP_011890446.1, NC_009337.1

Genome annotation databases

EnsemblBacteriaiABP37223; ABP37223; Cvib_1211
KEGGipvi:Cvib_1211

Similar proteinsi

Entry informationi

Entry nameiNADE_CHLPM
AccessioniPrimary (citable) accession number: A4SFG4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 15, 2007
Last modified: April 25, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health