A4SFC2 (ILVD_PROVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Cvib_1169

Organism

Prosthecochloris vibrioformis (strain DSM 265) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 265)) (Chlorobium phaeovibrioides (strain DSM 265)) [Complete proteome] [HAMAP]

Taxonomic identifier

290318 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000073982
Sites
	Metal binding	121	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	194	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A4SFC2 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 290198E3051BA764	FASTA	565	59,693
	10 20 30 40 50 60 MRSDTIKTGF EKAPHRSLLK ATGTIKEKGD YRKPFIGICN SFNELIPGHA HLQELGRIAK 70 80 90 100 110 120 EEVRKAGGVP FEFNTIGVCD GIAMGHIGMR YSLASRELIA DSVETVAEAH RLDGLVCIPN 130 140 150 160 170 180 CDKITPGMMM AALRINIPVI FVSGGPMKAG CTPSGKTVDL ISVFEAVGQH STGEISEQEL 190 200 210 220 230 240 ESIEENACPG CGSCSGMFTA NSMNCLSEAL GFALPGNGTI LAVDPRRNEL VREASRQIIT 250 260 270 280 290 300 LVKNNVRPRD ILSREALLNA FALDFAMGGS TNTILHTLAI ANEAELDFDF SELNALSART 310 320 330 340 350 360 PYICKVSPAT MAVHIEDVDR AGGISAILHE LSKVEGLLDL SVPTVTGHTL GENIADAEVK 370 380 390 400 410 420 DRSVIRSVED PYSATGGLCV LYGNLAPQGS VIKTGAVSAP MMKHTGPAKV YDCQDDAIKG 430 440 450 460 470 480 IMEGEVKSGD VVVIRYEGPK GGPGMPEMLS PTSVIMGRGL GDSVALITDG RFSGGSRGAC 490 500 510 520 530 540 IGHISPEAAE KGPIAALQNG DTITIDIPGR TISVDLTDED IASRIAALPD FQPKIKKGYL 550 560 ARYSQMVTSA STGAILKTPV SCEPK

References

[1]

"Complete sequence of Prosthecochloris vibrioformis DSM 265." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Mikhailova N., Li T., Overmann J., Schuster S.C., Bryant D.A., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 265.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000607 Genomic DNA. Translation: ABP37181.1.
RefSeq	YP_001130683.1. NC_009337.1.
3D structure databases
ProteinModelPortal	A4SFC2.
ModBase	Search...
Protein-protein interaction databases
STRING	A4SFC2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4970913.
GenomeReviews	Gene locus Cvib_1169 in contig CP000607_GR.
KEGG	pvi:Cvib_1169.
PATRIC	21396808. VBIChlPha132153_1234.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	SQDCRWP.
PhylomeDB	A4SFC2.
ProtClustDB	PRK00911.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_PROVI

Accession

Primary (citable) accession number: A4SFC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 15, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program