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A4SE28 (DDL_PROVI) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Cvib_0722
OrganismProsthecochloris vibrioformis (strain DSM 265) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 265)) (Chlorobium phaeovibrioides (strain DSM 265)) [Complete proteome] [HAMAP]
Taxonomic identifier290318 [NCBI]
Taxonomic lineageBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupChlorobium

Protein attributes

Sequence length361 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 361361D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000091205

Regions

Domain144 – 350207ATP-grasp
Nucleotide binding177 – 23256ATP By similarity

Sites

Metal binding3031Magnesium or manganese 1 By similarity
Metal binding3171Magnesium or manganese 1 By similarity
Metal binding3171Magnesium or manganese 2 By similarity
Metal binding3191Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A4SE28 [UniParc].

Last modified May 15, 2007. Version 1.
Checksum: 2F9C5F1878B36729

FASTA36138,898
        10         20         30         40         50         60 
MKQVTVALLF GGRSAEHEIS IISAQSVARH INPDHYRVVP IYITRNGRWY SEGIASSILE 

        70         80         90        100        110        120 
LDIAALIRKS GTAAASLKLQ EMVLASGQQP FSFTFAGIDI AFPILHGSYG EDGRIQGFLD 

       130        140        150        160        170        180 
TVAIPYTGCG VTASALTMDK ALSKLCVSEA GIAVAPGVTV LAGDWEKSPE QILSSIPETL 

       190        200        210        220        230        240 
IYPVFVKPAH LGSSVGISKV SVQGELPEAL AHACNLDTKV LIEQAMHGKE IEVAVLGNND 

       250        260        270        280        290        300 
PIASACGEIE PGSDFYDYDD KYIHNTAKLF IPARLPEELS RRVQADALTA YRALGCRGMA 

       310        320        330        340        350        360 
RVDFFVDEKS GQVVFNEINT IPGFTDISMY PQLMEAAGIA FPELCDRLLQ LALEPEQPAK 


T 

« Hide

References

[1]"Complete sequence of Prosthecochloris vibrioformis DSM 265."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Mikhailova N., Li T., Overmann J., Schuster S.C., Bryant D.A., Richardson P.
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 265.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000607 Genomic DNA. Translation: ABP36737.1.
RefSeqYP_001130239.1. NC_009337.1.

3D structure databases

ProteinModelPortalA4SE28.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290318.Cvib_0722.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP36737; ABP36737; Cvib_0722.
GeneID4970564.
KEGGpvi:Cvib_0722.
PATRIC21395820. VBIChlPha132153_0758.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG64BQ73.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycCPHA290318:GHNQ-737-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_PROVI
AccessionPrimary (citable) accession number: A4SE28
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 15, 2007
Last modified: February 19, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways