A4SDG1 (DXS_PROVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	Cvib_0498

Organism

Prosthecochloris vibrioformis (strain DSM 265) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 265)) (Chlorobium phaeovibrioides (strain DSM 265)) [Complete proteome] [HAMAP]

Taxonomic identifier

290318 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	635 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 635	635	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000079097

Sequences

	Sequence		Length	Mass (Da)
	A4SDG1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 9DCB70A4F6F634C1	FASTA	635	68,758
	10 20 30 40 50 60 MADSRSLLDT INSPRDLKKL SIRQLETLAN ECRHELINLI SLNGGHFASS LGVTELSVAL 70 80 90 100 110 120 HHVYNTEKDR IVWDVGHQAY IHKMLTGRRE RMNSNRKYGG ISGFPKIHES PHDAFGTGHA 130 140 150 160 170 180 STSISAAAGI ASARDLKGGN EKVIAVIGDG SMTGGMAFEA MNHLGDLKND VLVILNDNQM 190 200 210 220 230 240 AISPSTGGLK THMVNFTLNK TYNKARRLLW ESMSMMNNDL AERAKTSLRR LEDGMKAALT 250 260 270 280 290 300 PGAFFEALGL RYFGPIDGHN MGQLVRALRE MQELPHPKLL HVITTKGKGF LPAEENQSDW 310 320 330 340 350 360 HAHNGGFDTV TGITAKKEGP SATPKYQEVF GEALVEMALK DPAITAITAA MPTGTSLDLF 370 380 390 400 410 420 QKAMPDRFYD VGIAEGHAVT FAAGQALEGL KPVCAIYSTF LQRALDQVIH DVALQNLPVV 430 440 450 460 470 480 FAIDRAGLVG EDGPTHHGAF DLSYLHAVPG LTIMAPSDGQ ELRDMLHTAL YHIDGPVAIR 490 500 510 520 530 540 YPRGSTGGEE MRKNFTALEP GKGRMLKEGT GPVILTLGTM AATALEAGRL LENEGISVEI 550 560 570 580 590 600 ADMRFLKPLD TALIDRLSAS ATHIVTLEEN SIIGGFGSAV ADHLSEASKK TRLLRIGLPD 610 620 630 AFVTHGSMTD LYRETGLDAP AVAEKIRLFY TGRES

References

[1]

"Complete sequence of Prosthecochloris vibrioformis DSM 265." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Mikhailova N., Li T., Overmann J., Schuster S.C., Bryant D.A., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 265.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000607 Genomic DNA. Translation: ABP36520.1.
RefSeq	YP_001130022.1. NC_009337.1.
3D structure databases
ProteinModelPortal	A4SDG1.
ModBase	Search...
Protein-protein interaction databases
STRING	A4SDG1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4969543.
GenomeReviews	Gene locus Cvib_0498 in contig CP000607_GR.
KEGG	pvi:Cvib_0498.
PATRIC	21395348. VBIChlPha132153_0526.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1154.
HOGENOM	HBG571647.
OMA	DPILYHG.
PhylomeDB	A4SDG1.
ProtClustDB	PRK05444.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR011766. TPP_enzyme-bd_C. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_PROVI

Accession

Primary (citable) accession number: A4SDG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 15, 2007
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program