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A4RZ86

- LIAS_OSTLU

UniProt

A4RZ86 - LIAS_OSTLU

Protein

Lipoyl synthase, mitochondrial

Gene

LIP1

Organism
Ostreococcus lucimarinus (strain CCE9901)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (15 May 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi131 – 1311Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi136 – 1361Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi142 – 1421Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi162 – 1621Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi166 – 1661Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi169 – 1691Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    Name:LIP1UniRule annotation
    ORF Names:OSTLU_32294
    OrganismiOstreococcus lucimarinus (strain CCE9901)
    Taxonomic identifieri436017 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeChlorophytaMamiellophyceaeMamiellalesOstreococcus
    ProteomesiUP000001568: Chromosome 6

    Subcellular locationi

    Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3030MitochondrionUniRule annotationAdd
    BLAST
    Chaini31 – 399369Lipoyl synthase, mitochondrialPRO_0000398850Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi436017.A4RZ86.

    Structurei

    3D structure databases

    ProteinModelPortaliA4RZ86.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0320.
    KOiK03644.
    OMAiDHRLHSV.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    MF_03128. Lipoyl_synth_plantM.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR027527. Lipoyl_synth_mt.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A4RZ86-1 [UniParc]FASTAAdd to Basket

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    MRAVLELTRR RARNARFARA RAVVGARARA ADAQELRDDS KGGSSVDKAT    50
    STAAEARETA HTLEGFREAL RHGPGFDDFV RGDVAYSVPA PKSLKDKTTR 100
    KPAWLKREVP GGERYTEIKS KLRELKLATV CEEAKCPNLG ECWGGGDGKT 150
    ATATIMIMGD TCTRGCRFCA VKTAKAPPPL DKDEPANVSK AIAAWGLDYV 200
    VLTSVDRDDI EDQGAGHFRE TVQRLKASCD VLVEALTPDF RGEKHLVELV 250
    ATSGLDVFAH NVETVPELQR DVRDRRANWD QSIEVLKHAK KSGAKITKTS 300
    IMLGLGETHE QVVNALKLLR EADVDVVTFG QYMRPTKKHL AVVEYVTPEA 350
    FKRYQEIAEE MGFLYVASGA MVRSSYKAGE FFLANVIKQR KAKEAAAAN 399
    Length:399
    Mass (Da):43,907
    Last modified:May 15, 2007 - v1
    Checksum:i2D648EC52BE8F0E5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000586 Genomic DNA. Translation: ABO96579.1.
    RefSeqiXP_001418286.1. XM_001418249.1.

    Genome annotation databases

    GeneIDi5002530.
    KEGGiolu:OSTLU_32294.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000586 Genomic DNA. Translation: ABO96579.1 .
    RefSeqi XP_001418286.1. XM_001418249.1.

    3D structure databases

    ProteinModelPortali A4RZ86.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 436017.A4RZ86.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 5002530.
    KEGGi olu:OSTLU_32294.

    Phylogenomic databases

    eggNOGi COG0320.
    KOi K03644.
    OMAi DHRLHSV.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    MF_03128. Lipoyl_synth_plantM.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR027527. Lipoyl_synth_mt.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CCE9901.

    Entry informationi

    Entry nameiLIAS_OSTLU
    AccessioniPrimary (citable) accession number: A4RZ86
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: May 15, 2007
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3