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A4RZ86

- LIAS_OSTLU

UniProt

A4RZ86 - LIAS_OSTLU

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Protein

Lipoyl synthase, mitochondrial

Gene

LIP1

Organism
Ostreococcus lucimarinus (strain CCE9901)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi131 – 1311Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi136 – 1361Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi142 – 1421Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi162 – 1621Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi166 – 1661Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi169 – 1691Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. aromatic amino acid family biosynthetic process Source: EnsemblPlants/Gramene
  2. cell differentiation Source: EnsemblPlants/Gramene
  3. chlorophyll biosynthetic process Source: EnsemblPlants/Gramene
  4. cysteine biosynthetic process Source: EnsemblPlants/Gramene
  5. glycine catabolic process Source: EnsemblPlants/Gramene
  6. isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway Source: EnsemblPlants/Gramene
  7. jasmonic acid biosynthetic process Source: EnsemblPlants/Gramene
  8. leaf morphogenesis Source: EnsemblPlants/Gramene
  9. nucleotide metabolic process Source: EnsemblPlants/Gramene
  10. oxidoreduction coenzyme metabolic process Source: EnsemblPlants/Gramene
  11. positive regulation of transcription, DNA-templated Source: EnsemblPlants/Gramene
  12. protein lipoylation Source: UniProtKB-HAMAP
  13. secondary metabolic process Source: EnsemblPlants/Gramene
  14. unsaturated fatty acid biosynthetic process Source: EnsemblPlants/Gramene
  15. vitamin metabolic process Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
Name:LIP1UniRule annotation
ORF Names:OSTLU_32294
OrganismiOstreococcus lucimarinus (strain CCE9901)
Taxonomic identifieri436017 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaprasinophytesMamiellophyceaeMamiellalesBathycoccaceaeOstreococcus
ProteomesiUP000001568: Chromosome 6

Subcellular locationi

Mitochondrion UniRule annotation

GO - Cellular componenti

  1. mitochondrial matrix Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3030MitochondrionUniRule annotationAdd
BLAST
Chaini31 – 399369Lipoyl synthase, mitochondrialPRO_0000398850Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi436017.A4RZ86.

Structurei

3D structure databases

ProteinModelPortaliA4RZ86.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
KOiK03644.
OMAiDHRLHSV.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
MF_03128. Lipoyl_synth_plantM.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027527. Lipoyl_synth_mt.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A4RZ86-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRAVLELTRR RARNARFARA RAVVGARARA ADAQELRDDS KGGSSVDKAT
60 70 80 90 100
STAAEARETA HTLEGFREAL RHGPGFDDFV RGDVAYSVPA PKSLKDKTTR
110 120 130 140 150
KPAWLKREVP GGERYTEIKS KLRELKLATV CEEAKCPNLG ECWGGGDGKT
160 170 180 190 200
ATATIMIMGD TCTRGCRFCA VKTAKAPPPL DKDEPANVSK AIAAWGLDYV
210 220 230 240 250
VLTSVDRDDI EDQGAGHFRE TVQRLKASCD VLVEALTPDF RGEKHLVELV
260 270 280 290 300
ATSGLDVFAH NVETVPELQR DVRDRRANWD QSIEVLKHAK KSGAKITKTS
310 320 330 340 350
IMLGLGETHE QVVNALKLLR EADVDVVTFG QYMRPTKKHL AVVEYVTPEA
360 370 380 390
FKRYQEIAEE MGFLYVASGA MVRSSYKAGE FFLANVIKQR KAKEAAAAN
Length:399
Mass (Da):43,907
Last modified:May 15, 2007 - v1
Checksum:i2D648EC52BE8F0E5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000586 Genomic DNA. Translation: ABO96579.1.
RefSeqiXP_001418286.1. XM_001418249.1.

Genome annotation databases

EnsemblPlantsiABO96579; ABO96579; OSTLU_32294.
GeneIDi5002530.
KEGGiolu:OSTLU_32294.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000586 Genomic DNA. Translation: ABO96579.1 .
RefSeqi XP_001418286.1. XM_001418249.1.

3D structure databases

ProteinModelPortali A4RZ86.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 436017.A4RZ86.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi ABO96579 ; ABO96579 ; OSTLU_32294 .
GeneIDi 5002530.
KEGGi olu:OSTLU_32294.

Phylogenomic databases

eggNOGi COG0320.
KOi K03644.
OMAi DHRLHSV.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
MF_03128. Lipoyl_synth_plantM.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027527. Lipoyl_synth_mt.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CCE9901.

Entry informationi

Entry nameiLIAS_OSTLU
AccessioniPrimary (citable) accession number: A4RZ86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: May 15, 2007
Last modified: October 29, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3