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Protein

Lipoyl synthase, mitochondrial

Gene

LIP1

Organism
Ostreococcus lucimarinus (strain CCE9901)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi131 – 1311Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi136 – 1361Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi142 – 1421Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi162 – 1621Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi166 – 1661Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi169 – 1691Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
Name:LIP1UniRule annotation
ORF Names:OSTLU_32294
OrganismiOstreococcus lucimarinus (strain CCE9901)
Taxonomic identifieri436017 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaprasinophytesMamiellophyceaeMamiellalesBathycoccaceaeOstreococcus
ProteomesiUP000001568 Componenti: Chromosome 6

Subcellular locationi

  • Mitochondrion UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3030MitochondrionUniRule annotationAdd
BLAST
Chaini31 – 399369Lipoyl synthase, mitochondrialPRO_0000398850Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi436017.A4RZ86.

Structurei

3D structure databases

ProteinModelPortaliA4RZ86.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
KOiK03644.
OMAiCAFCQVE.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
MF_03128. Lipoyl_synth_plantM.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027527. Lipoyl_synth_mt.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A4RZ86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAVLELTRR RARNARFARA RAVVGARARA ADAQELRDDS KGGSSVDKAT
60 70 80 90 100
STAAEARETA HTLEGFREAL RHGPGFDDFV RGDVAYSVPA PKSLKDKTTR
110 120 130 140 150
KPAWLKREVP GGERYTEIKS KLRELKLATV CEEAKCPNLG ECWGGGDGKT
160 170 180 190 200
ATATIMIMGD TCTRGCRFCA VKTAKAPPPL DKDEPANVSK AIAAWGLDYV
210 220 230 240 250
VLTSVDRDDI EDQGAGHFRE TVQRLKASCD VLVEALTPDF RGEKHLVELV
260 270 280 290 300
ATSGLDVFAH NVETVPELQR DVRDRRANWD QSIEVLKHAK KSGAKITKTS
310 320 330 340 350
IMLGLGETHE QVVNALKLLR EADVDVVTFG QYMRPTKKHL AVVEYVTPEA
360 370 380 390
FKRYQEIAEE MGFLYVASGA MVRSSYKAGE FFLANVIKQR KAKEAAAAN
Length:399
Mass (Da):43,907
Last modified:May 15, 2007 - v1
Checksum:i2D648EC52BE8F0E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000586 Genomic DNA. Translation: ABO96579.1.
RefSeqiXP_001418286.1. XM_001418249.1.

Genome annotation databases

EnsemblPlantsiABO96579; ABO96579; OSTLU_32294.
GeneIDi5002530.
KEGGiolu:OSTLU_32294.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000586 Genomic DNA. Translation: ABO96579.1.
RefSeqiXP_001418286.1. XM_001418249.1.

3D structure databases

ProteinModelPortaliA4RZ86.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi436017.A4RZ86.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiABO96579; ABO96579; OSTLU_32294.
GeneIDi5002530.
KEGGiolu:OSTLU_32294.

Phylogenomic databases

eggNOGiCOG0320.
KOiK03644.
OMAiCAFCQVE.

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
MF_03128. Lipoyl_synth_plantM.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027527. Lipoyl_synth_mt.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CCE9901.

Entry informationi

Entry nameiLIAS_OSTLU
AccessioniPrimary (citable) accession number: A4RZ86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: May 15, 2007
Last modified: April 1, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.