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A4RZ86

- LIAS_OSTLU

UniProt

A4RZ86 - LIAS_OSTLU

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Protein

Lipoyl synthase, mitochondrial

Gene
LIP1, OSTLU_32294
Organism
Ostreococcus lucimarinus (strain CCE9901)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi131 – 1311Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi136 – 1361Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi142 – 1421Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi162 – 1621Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi166 – 1661Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi169 – 1691Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrial (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase
Short name:
LS
Short name:
Lip-syn
Lipoic acid synthase
Gene namesi
Name:LIP1
ORF Names:OSTLU_32294
OrganismiOstreococcus lucimarinus (strain CCE9901)
Taxonomic identifieri436017 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaMamiellophyceaeMamiellalesOstreococcus
ProteomesiUP000001568: Chromosome 6

Subcellular locationi

Mitochondrion Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3030Mitochondrion Reviewed predictionAdd
BLAST
Chaini31 – 399369Lipoyl synthase, mitochondrialUniRule annotationPRO_0000398850Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi436017.A4RZ86.

Structurei

3D structure databases

ProteinModelPortaliA4RZ86.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
KOiK03644.
OMAiDHRLHSV.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
MF_03128. Lipoyl_synth_plantM.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027527. Lipoyl_synth_mt.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A4RZ86-1 [UniParc]FASTAAdd to Basket

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MRAVLELTRR RARNARFARA RAVVGARARA ADAQELRDDS KGGSSVDKAT    50
STAAEARETA HTLEGFREAL RHGPGFDDFV RGDVAYSVPA PKSLKDKTTR 100
KPAWLKREVP GGERYTEIKS KLRELKLATV CEEAKCPNLG ECWGGGDGKT 150
ATATIMIMGD TCTRGCRFCA VKTAKAPPPL DKDEPANVSK AIAAWGLDYV 200
VLTSVDRDDI EDQGAGHFRE TVQRLKASCD VLVEALTPDF RGEKHLVELV 250
ATSGLDVFAH NVETVPELQR DVRDRRANWD QSIEVLKHAK KSGAKITKTS 300
IMLGLGETHE QVVNALKLLR EADVDVVTFG QYMRPTKKHL AVVEYVTPEA 350
FKRYQEIAEE MGFLYVASGA MVRSSYKAGE FFLANVIKQR KAKEAAAAN 399
Length:399
Mass (Da):43,907
Last modified:May 15, 2007 - v1
Checksum:i2D648EC52BE8F0E5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000586 Genomic DNA. Translation: ABO96579.1.
RefSeqiXP_001418286.1. XM_001418249.1.

Genome annotation databases

GeneIDi5002530.
KEGGiolu:OSTLU_32294.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000586 Genomic DNA. Translation: ABO96579.1 .
RefSeqi XP_001418286.1. XM_001418249.1.

3D structure databases

ProteinModelPortali A4RZ86.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 436017.A4RZ86.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 5002530.
KEGGi olu:OSTLU_32294.

Phylogenomic databases

eggNOGi COG0320.
KOi K03644.
OMAi DHRLHSV.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
MF_03128. Lipoyl_synth_plantM.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027527. Lipoyl_synth_mt.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CCE9901.

Entry informationi

Entry nameiLIAS_OSTLU
AccessioniPrimary (citable) accession number: A4RZ86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: May 15, 2007
Last modified: May 14, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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