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Protein

Lipoyl synthase, mitochondrial

Gene

LIP1

Organism
Ostreococcus lucimarinus (strain CCE9901)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Lipoyl synthase, chloroplastic (LIP1P), Lipoyl synthase, mitochondrial (LIP1)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi131Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi136Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi142Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi162Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi166Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi169Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
Name:LIP1UniRule annotation
ORF Names:OSTLU_32294
OrganismiOstreococcus lucimarinus (strain CCE9901)
Taxonomic identifieri436017 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaprasinophytesMamiellophyceaeMamiellalesBathycoccaceaeOstreococcus
Proteomesi
  • UP000001568 Componenti: Chromosome 6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 30MitochondrionUniRule annotationAdd BLAST30
ChainiPRO_000039885031 – 399Lipoyl synthase, mitochondrialAdd BLAST369

Interactioni

Protein-protein interaction databases

STRINGi436017.A4RZ86

Structurei

3D structure databases

ProteinModelPortaliA4RZ86
SMRiA4RZ86
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2672 Eukaryota
COG0320 LUCA
KOiK03644
OMAiGRCPNRG

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
MF_03128 Lipoyl_synth_plantM, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR031691 LIAS_N
IPR003698 Lipoyl_synth
IPR027527 Lipoyl_synth_mt
IPR007197 rSAM
PfamiView protein in Pfam
PF16881 LIAS_N, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A4RZ86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAVLELTRR RARNARFARA RAVVGARARA ADAQELRDDS KGGSSVDKAT
60 70 80 90 100
STAAEARETA HTLEGFREAL RHGPGFDDFV RGDVAYSVPA PKSLKDKTTR
110 120 130 140 150
KPAWLKREVP GGERYTEIKS KLRELKLATV CEEAKCPNLG ECWGGGDGKT
160 170 180 190 200
ATATIMIMGD TCTRGCRFCA VKTAKAPPPL DKDEPANVSK AIAAWGLDYV
210 220 230 240 250
VLTSVDRDDI EDQGAGHFRE TVQRLKASCD VLVEALTPDF RGEKHLVELV
260 270 280 290 300
ATSGLDVFAH NVETVPELQR DVRDRRANWD QSIEVLKHAK KSGAKITKTS
310 320 330 340 350
IMLGLGETHE QVVNALKLLR EADVDVVTFG QYMRPTKKHL AVVEYVTPEA
360 370 380 390
FKRYQEIAEE MGFLYVASGA MVRSSYKAGE FFLANVIKQR KAKEAAAAN
Length:399
Mass (Da):43,907
Last modified:May 15, 2007 - v1
Checksum:i2D648EC52BE8F0E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000586 Genomic DNA Translation: ABO96579.1
RefSeqiXP_001418286.1, XM_001418249.1

Genome annotation databases

EnsemblPlantsiABO96579; ABO96579; OSTLU_32294
GeneIDi5002530
GrameneiABO96579; ABO96579; OSTLU_32294
KEGGiolu:OSTLU_32294

Similar proteinsi

Entry informationi

Entry nameiLIAS_OSTLU
AccessioniPrimary (citable) accession number: A4RZ86
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: May 15, 2007
Last modified: May 23, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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