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Protein

Dicer-like protein 1

Gene

DCL1

Organism
Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Dicer-like endonuclease which seems not to be involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway, contrary to its DCL2 counterpart.1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi1283 – 12831Magnesium or manganeseBy similarity
Sitei1388 – 13881Important for activityBy similarity
Metal bindingi1392 – 13921Magnesium or manganeseBy similarity
Metal bindingi1395 – 13951Magnesium or manganeseBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi128 – 1358ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antiviral protein, Helicase, Hydrolase

Keywords - Biological processi

Antiviral defense

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Dicer-like protein 1
Including the following 2 domains:
Endoribonuclease DCL1 (EC:3.1.26.-)
ATP-dependent helicase DCL1 (EC:3.6.4.-)
Gene namesi
Name:DCL1
Synonyms:MDL1
ORF Names:MGG_01541
OrganismiMagnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Taxonomic identifieri242507 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeMagnaporthalesMagnaporthaceaeMagnaporthe
Proteomesi
  • UP000009058 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiFungiDB:MGG_01541.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15911591Dicer-like protein 1PRO_0000306781Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliA4RKC3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini115 – 298184Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini439 – 607169Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini639 – 72991Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd
BLAST
Domaini1050 – 1190141RNase III 1PROSITE-ProRule annotationAdd
BLAST
Domaini1243 – 1406164RNase III 2PROSITE-ProRule annotationAdd
BLAST
Domaini1440 – 151475DRBMAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi242 – 2454DEAH box

Sequence similaritiesi

Belongs to the helicase family. Dicer subfamily.PROSITE-ProRule annotation
Contains 1 Dicer dsRNA-binding fold domain.PROSITE-ProRule annotation
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 2 RNase III domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

InParanoidiA4RKC3.
KOiK11592.
OrthoDBiEOG76QFRP.

Family and domain databases

Gene3Di1.10.1520.10. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR005034. Dicer_dimerisation_dom.
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 3 hits.
PROSITEiPS51327. DICER_DSRBF. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4RKC3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVHDGLKSP DKAAKSRYDD DRIDQDSEDE AVRLVANPDP SKPRKISERK
60 70 80 90 100
RADQAAFESW VNDNKIRLSL PSATKSRRSG IRELFSSDET LETRPTRTRP
110 120 130 140 150
RERVIEGPRE YQIELFERAK QKNTIAVLDT GTGKTLIAIL LIRHIIELEL
160 170 180 190 200
GARWQGREKR ITFFLVDKVA LVRQQTDHIR ANLDFPVTGL HGDTVRNLWY
210 220 230 240 250
SKEYFEKLLQ EQEVVVCTAE ILYRCLHRSY LNISQVSLVV FDEAHHAKKN
260 270 280 290 300
HVYARIIKDF YLMEEDCQKR PRIFGMTASP IDTKDTCISY ERATHELESL
310 320 330 340 350
LHSEIATISD RDLLKVIGSR PQEVRKSYAR VLRQENTELC NQLRELVGNH
360 370 380 390 400
PLFKQTFDSA EFAVTELGAW CADKLWELCF REEAVSTLDG RVEGSRARDP
410 420 430 440 450
DEVGESSHEV SNAREALSLV QQWSFSPPED GSLSTKTHKL IEILAECFSQ
460 470 480 490 500
ASAGNAIQCI VFVKRRDTAV LLNALCEQAE IRTKIPDLKG AFLIGAGRGG
510 520 530 540 550
NAAFTTTRQQ EQTVSRFRDG EINCLFATSI AEEGLDIPGC NVVIRFDLHG
560 570 580 590 600
TTIQYIQSRG RARMRNSWFI HMTEFGNPEH NRRWFQDRVN EQKMRDFCLS
610 620 630 640 650
LPKDRIMEKA EVDDVLRGDQ SQKIFVVPGS KASLTFKQSL VVLAEFVATL
660 670 680 690 700
PARPDEILSV DYTVVPVFGG FQGEVYLPAS SPLRSAMGGV YRSKQLAKCA
710 720 730 740 750
AAYAMCIQLY NSNYLDDHLK STLAKVLPAM RNARLAVSSK KRKSYNMRTK
760 770 780 790 800
PVLWSEVGPL TELYAMVLSL AQPGAAYYHS RPILLLTRKP LPEIAQFPLF
810 820 830 840 850
FGKGSSRRSN VRCIPLAHPW SPTTTQVEGI CAFTLCIFRD IFSKDFQASG
860 870 880 890 900
TDMPYFLAPS TGIEHGTDLS SLVNPERIID WATVHRTTTT DRVPYNFNEP
910 920 930 940 950
DEFFQDKYVS DPFDGSRKFF MRKVRRDLKP QDKVPEGVPA PSKWRAVEHT
960 970 980 990 1000
ILNYSVSLWK KSRAGHNSRQ DQPVVEAELA PLRRNLLDET DGENSTGPQT
1010 1020 1030 1040 1050
CYLVLETLLI SQIPVDTVVM AYTFPAIIYR LENSLISLEA CQNLGLDIPI
1060 1070 1080 1090 1100
DIALIAMTKD SDNSDDHDEA PINFQSGMGQ NYERLEFLGD CFLKMATSIA
1110 1120 1130 1140 1150
LYTLVEGDEF EYHVERMLDI CNKNLLNWAL ESNLQEHIRS KSFNRRTWYP
1160 1170 1180 1190 1200
PGLKLLKGKK TEVDDEHALG DKSIADVCEA LIGAAYLTAQ AQSSPNFDLA
1210 1220 1230 1240 1250
VKAVTVMTHS KTHTMQAWSD YYASYQCPEW LSTPPSQTQL ELCSQIKNKM
1260 1270 1280 1290 1300
GYRFKNPRLL RCAFMHPSYP RQYENIPSYQ RLEFLGDSLL DMVCVDYLFK
1310 1320 1330 1340 1350
KHPDKDPQWL TEHKMAMVSN QFFGCVAVGL GFHRHLIHMQ PALGGSITEW
1360 1370 1380 1390 1400
AELVTKKREE ARQLAVRRGQ REEDYARDFW IEVHHPPKCL PDILEAYVGA
1410 1420 1430 1440 1450
LFVDTGYDYS AVVGFFDRHI KPYFADMSIY DMYSSKHPVT HITSIITTQF
1460 1470 1480 1490 1500
GCSSFRLMVH EIPDDVQGEG LVTGAVKVVA ACMIHGEVRC HAVAASGRYA
1510 1520 1530 1540 1550
KLAVAKQAVA IYEDMSPTEF RLRHGCNCKP EDGDGDHGVL VDHRADCEPE
1560 1570 1580 1590
EPLRKQDPPA KSVVDVDGKT IAEMRLRHSK QMNDQEECLS W
Length:1,591
Mass (Da):180,666
Last modified:October 2, 2007 - v2
Checksum:iBFB08E2247DD9A84
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM001232 Genomic DNA. Translation: EHA54708.1.
RefSeqiXP_003714515.1. XM_003714467.1.

Genome annotation databases

EnsemblFungiiMGG_01541T0; MGG_01541T0; MGG_01541.
GeneIDi2679399.
KEGGimgr:MGG_01541.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM001232 Genomic DNA. Translation: EHA54708.1.
RefSeqiXP_003714515.1. XM_003714467.1.

3D structure databases

ProteinModelPortaliA4RKC3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiMGG_01541T0; MGG_01541T0; MGG_01541.
GeneIDi2679399.
KEGGimgr:MGG_01541.

Organism-specific databases

EuPathDBiFungiDB:MGG_01541.

Phylogenomic databases

InParanoidiA4RKC3.
KOiK11592.
OrthoDBiEOG76QFRP.

Family and domain databases

Gene3Di1.10.1520.10. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR005034. Dicer_dimerisation_dom.
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 3 hits.
PROSITEiPS51327. DICER_DSRBF. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 70-15 / ATCC MYA-4617 / FGSC 8958.
  2. "One of the two Dicer-like proteins in the filamentous fungi Magnaporthe oryzae genome is responsible for hairpin RNA-triggered RNA silencing and related small interfering RNA accumulation."
    Kadotani N., Nakayashiki H., Tosa Y., Mayama S.
    J. Biol. Chem. 279:44467-44474(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiDCL1_MAGO7
AccessioniPrimary (citable) accession number: A4RKC3
Secondary accession number(s): G4MTF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: February 17, 2016
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.