Reviewed, UniProtKB/Swiss-Prot A4RF25 (PMIP_MAGGR)

Names and origin

Protein names

Recommended name:
    Mitochondrial intermediate peptidase
      Short name=MIP
    EC=3.4.24.59
Alternative name(s):
    Octapeptidyl aminopeptidase

Gene names

Name:	OCT1
ORF Names:	MGG_00487

Organism

Magnaporthe grisea (Rice blast fungus) (Pyricularia grisea) [Complete proteome]

Taxonomic identifier

148305 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Magnaporthales › Magnaporthaceae › Magnaporthe

Protein attributes

Sequence length	812 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane By similarity.
Catalytic activity	Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.
Cofactor	Binds 1 zinc ion By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the peptidase M3 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 35	35	Mitochondrion Potential
	Chain	36 – 812	777	Mitochondrial intermediate peptidase		PRO_0000338587
Regions
	Compositional bias	296 – 299	4	Poly-Ala
Sites
	Active site	588	1	By similarity
	Metal binding	587	1	Zinc; catalytic By similarity
	Metal binding	591	1	Zinc; catalytic By similarity
	Metal binding	594	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4RF25-1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: ADBC3C60189F33E9	FASTA	812	90,362
	10 20 30 40 50 60 MLKLLRPRPW VCNSCLNRVA FPKPYPVGSR STRWLSTAQS AAPAVSIPPV NPVPADHTTS 70 80 90 100 110 120 GTHDDALLGK IFDCSSAWRD FSARSAKFPT ENAGLFRNAY LTSPDGFLTF AQSSLSKASA 130 140 150 160 170 180 IVNRVLGAST IEEYKTIVRD LDRLSDLLCR VIDLSDFVRV THPDVRIQRA ASEAWYMVYQ 190 200 210 220 230 240 YMNQLNTMTG LNDQLGKAME NSDVTKTWSE EEMAVAQLLK LDFMKSAVNL PQAARDRFVD 250 260 270 280 290 300 LSQRISEIGS DFVNEMAPEQ RRVVLPSSKF QGMDPQIARR FTKHGYMQLP TMSGEAAAAL 310 320 330 340 350 360 RTVHDEETRK AVYLAIRTAS SRSVGLLEAL LKHRAELADL AGFESYGHMT LRDRMMAKTP 370 380 390 400 410 420 ESINKFLVEL SKNNAPRVLQ EVDSLLQEKK TLLASPSATL NPWDREYYIQ RIRNAQGKNV 430 440 450 460 470 480 KHDNFFASYF SVGRVMQGLS RLFTRLYGIR FVPRETLPGE KWHPDVRRLD VVSDTDGHVA 490 500 510 520 530 540 VLYCDLFYRE DKSPNPAHFT IRCSRAISED EISEAAVSTS EGGPAFGSPE SAANDGMAAS 550 560 570 580 590 600 RGASGGPLKQ LPTIALVCDF PQRDNPLSGS KSKPASLTFA SLETLFHEMG HAIHSVLART 610 620 630 640 650 660 SFQNVAGTRC ATDLAELPST LMEYFASDPS VLSLFARHAE TDEPLDYDLL AERVRSRGRF 670 680 690 700 710 720 EGCDTDYQII LAMLDQAYHS PLASSESFDT TRAYHDLQRE HSPLGPDPSS TRWQGFFGHL 730 740 750 760 770 780 FGYGSTYYSY LFDQVLAERA WKKVFSSGQD GAALSREAGE HLKESLLKWG GSREPWRCVS 790 800 810 DVLRDERIAG GGEEAMALVG SWGTSNKSTM KH

References

[1]

"The genome sequence of the rice blast fungus Magnaporthe grisea." Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K., Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D., Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S., Soanes D.M. , Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M., Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E., Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W. Nature 434:980-986(2005) [PubMed: 15846337] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 70-15 / FGSC 8958.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH476836 Genomic DNA. Translation: EDK02896.1.
RefSeq	XP_368757.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M03.006.
Genome annotation databases
GeneID	2674708.
KEGG	mgr:MGG_00487.
NMPDR	fig|242507.1.peg.674.
Phylogenomic databases
OMA	AMGERYR.
Family and domain databases
InterPro	IPR001567. Pept_M3A_M3B. IPR006025. Pept_M_Zn_BS. [Graphical view]
Pfam	PF01432. Peptidase_M3. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMIP_MAGGR

Accession

Primary (citable) accession number: A4RF25

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	May 15, 2007
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)