A4RAE9 (AMPP3_MAGO7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable Xaa-Pro aminopeptidase PEPP
EC=3.4.11.9

Alternative name(s):
Aminoacylproline aminopeptidase

Gene names

Name:	PEPP
ORF Names:	MGG_13438

Organism

Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae) [Complete proteome]

Taxonomic identifier

242507 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Sordariomycetidae › Magnaporthales › Magnaporthaceae › Magnaporthe › Magnaporthe oryzae

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides By similarity.
Catalytic activity	Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Sequence similarities	Belongs to the peptidase M24B family.
Sequence caution	The sequence EHA51744.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular process Inferred from electronic annotation. Source: InterPro mycelium development Inferred from expression pattern PubMed 17156450. Source: PAMGO_MGG proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 465	465	Probable Xaa-Pro aminopeptidase PEPP		PRO_0000411874
Sites
	Metal binding	259	1	Manganese 2 By similarity
	Metal binding	270	1	Manganese 1 By similarity
	Metal binding	270	1	Manganese 2 By similarity
	Metal binding	395	1	Manganese 1 By similarity
	Metal binding	435	1	Manganese 1 By similarity
	Metal binding	435	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4RAE9 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 9F9A8FC38B5B44E9	FASTA	465	51,824
	10 20 30 40 50 60 MASKNYDDVL KGKYPAKEHA RRVVEVIRSS QPDVSGVLYL EGQKTKMIED NDSEEHFRQR 70 80 90 100 110 120 RYFYYLTGCE LPDCYLTYDI ATSRSTLYIP PVDPESVIWS GLPMSALEAL QKYDVDEVRY 130 140 150 160 170 180 THEVNAALTS LAEAAPSSTV YAIPNQVSDS ITFLGFGAKN FDVLKPAIER ARVVKSDYEI 190 200 210 220 230 240 ALIAKANDIS GAAHLAVLKR VRHVSNEREL YATFLAECIS RGAPHMAYHS IVAAGRAAAT 250 260 270 280 290 300 LHYVKNDEPT AGKLNLLLDA ACELNCYASD ITRTFPISGS FTPESRAIYD TVLRMQLETL 310 320 330 340 350 360 AMLKEGVRWD DVHIHAHRVA IEGLLAAGIF KKGFSVDEIL ESRTSVAFFP HGLGHYLGMD 370 380 390 400 410 420 THDTGGNANY QDKDSMFRYL RVRGTLPAGS VITVEPGIYF CNFIIEPYLK DEKHSKYIDA 430 440 450 460 AVLDKYWDVG GVRIEDNVVI TKDGYDNLTT AVKDAKEMEK IISSS

References

[1]

"The genome sequence of the rice blast fungus Magnaporthe grisea." Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K., Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D., Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S., Soanes D.M. , Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M., Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E., Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W. Nature 434:980-986(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 70-15 / ATCC MYA-4617 / FGSC 8958.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CM001233 Genomic DNA. Translation: EHA51744.1. Different initiation.
RefSeq	XP_003711551.1. XM_003711503.1.
3D structure databases
ProteinModelPortal	A4RAE9.
ModBase	Search...
Protein-protein interaction databases
STRING	148305.A4RAE9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5050362.
KEGG	mgr:MGG_13438.
Phylogenomic databases
KO	K14213.
OrthoDB	EOG4J40RD.
Family and domain databases
Gene3D	3.90.230.10. 1 hit.
InterPro	IPR007865. Aminopep_P_N. IPR000994. Pept_M24_structural-domain. [Graphical view]
Pfam	PF05195. AMP_N. 1 hit. PF00557. Peptidase_M24. 1 hit. [Graphical view]
SMART	SM01011. AMP_N. 1 hit. [Graphical view]
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
PROSITE	PS00491. PROLINE_PEPTIDASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPP3_MAGO7

Accession

Primary (citable) accession number: A4RAE9
Secondary accession number(s): G4N2Y5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	May 15, 2007
Last modified:	April 3, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program