Reviewed, UniProtKB/Swiss-Prot A4R935 (NCB5R_MAGGR)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase 1
    EC=1.6.2.2
Alternative name(s):
    Microsomal cytochrome b reductase

Gene names

Name:	CBR1
ORF Names:	MGG_06289

Organism

Magnaporthe grisea (Rice blast fungus) (Pyricularia grisea) [Complete proteome]

Taxonomic identifier

148305 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Magnaporthales › Magnaporthaceae › Magnaporthe

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Electron donor reductase for cytochrome b5. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein By similarity. Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	NADH-cytochrome b5 reductase 1		PRO_0000330157
Regions
	Transmembrane	31 – 51	21	Potential
	Domain	60 – 168	109	FAD-binding FR-type
	Nucleotide binding	148 – 163	16	FAD By similarity
	Nucleotide binding	174 – 208	35	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4R935-1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: DB354E0C02610802	FASTA	309	33,630
	10 20 30 40 50 60 MAPPLSSKVY VDGVYIPAAL IVIGTAIVKR DWVVYSVALA LALGTWKFFQ LKPKKVLDPT 70 80 90 100 110 120 KFQEFELKEK TIISHNVAIY RIQLPSPSSI LGLPIGQHIS IGADIPQPDG SSKEVVRSYT 130 140 150 160 170 180 PISGDEQPGY VDLLIKSYPT GNISKYMAGL SVGQSIRVRG PKGAFVYQPN MVRHFGMIAG 190 200 210 220 230 240 GTGITPMLQV VRAIVRGRAA GDTTQVDLIF ANVTKEDILL KEDLDALAAE DKGFRVHYVL 250 260 270 280 290 300 DRPPEGWTGG VGFVTQDMIT KWLPKPADDV KILLCGPPPM VSGLKKATEA LGFKKARPVS KLEDQVFAF

References

[1]

"The genome sequence of the rice blast fungus Magnaporthe grisea." Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K., Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D., Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S., Soanes D.M. , Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M., Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E., Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W. Nature 434:980-986(2005) [PubMed: 15846337] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 70-15 / FGSC 8958.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH476833 Genomic DNA. Translation: EDK00805.1.
RefSeq	XP_369774.1.
UniGene	Mgr.9835
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2684444.
KEGG	mgr:MGG_06289.
NMPDR	fig|242507.1.peg.10352.
Phylogenomic databases
OMA	GNISRHV.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCB5R_MAGGR

Accession

Primary (citable) accession number: A4R935

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	May 15, 2007
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)