Reviewed, UniProtKB/Swiss-Prot A4R5S9 (KATG1_MAGGR)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 1
      Short name=CP 1
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 1

Gene names

Name:	katG1
Synonyms:	MagKatG1
ORF Names:	MGG_04337

Organism

Magnaporthe grisea (Rice blast fungus) (Pyricularia grisea) [Complete proteome]

Taxonomic identifier

148305 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Magnaporthales › Magnaporthaceae › Magnaporthe

Protein attributes

Sequence length	750 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. Donor + H2O2 = oxidized donor + 2 H2O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 750	750	Catalase-peroxidase 1		PRO_0000345090
Sites
	Active site	91	1	Proton acceptor By similarity
	Metal binding	279	1	Iron (heme axial ligand) By similarity
	Site	87	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	90 ↔ 238		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-264) By similarity
	Cross-link	238 ↔ 264		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-90) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4R5S9-1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: E327E7C3950B46DE	FASTA	750	82,859
	10 20 30 40 50 60 MGECPLRTAN VAGGGTRNRD WWPNTLKLNI LRQHTEATNP YDPNFDYAEA FKSLDYEGLK 70 80 90 100 110 120 KDLRALMTDS QEYWPADFGH YGGLFVRMAW HSAGTYRVMD GRGGGGQGQQ RFAPLNSWPD 130 140 150 160 170 180 NVSLDKARRL LWPIKQKYGN KISWADLMLL TGNVALEDMG FKTFGFAGGR PDTWEADEST 190 200 210 220 230 240 YWGGETTWLG NEVRYSSGNE GHKESGVIDG SESKKGHKDI HTRDLEKPVS AAHMGLIYVN 250 260 270 280 290 300 PEGPDGIPDP VAAARDIRTT FSRMAMNDEE TVALIAGGHT VGKTHGAAPS DNVGPEPEAA 310 320 330 340 350 360 PIENQGLGWS NKHGSGKGPD TITSGLEVIW TKEPAKFTMN YLEYLFKYEW ELTKSPAGAN 370 380 390 400 410 420 QWVAKNAEEF IPDAFDPSKK HKPRMLTTDL SLRFDPEYEK ISRRFLENPE QFKDAFARAW 430 440 450 460 470 480 FKLLHRDMGP RSRWLGPEVP KETLLWEDPI PTPDHPIIDG SDVDSLKKAI LATGVAPSKL 490 500 510 520 530 540 IQTAWASAST FRGGDKRGGA NGARIRLEPQ NKWEVNNPQQ LAEVLKALEG VKADFEKSGK 550 560 570 580 590 600 KVSIADLIVL AGVAAVEQAA GVPVPFTPGR GDATQEQTDV ESFTHLEPAA DAFRNYGKGT 610 620 630 640 650 660 SRVTTEQIMV DRAQQLTLTA PELTVLVGGL RVLGANYDGS SHGVWTDKPG KLTNDFFVTL 670 680 690 700 710 720 LDPYTSWKSV DGEVFEGTNS KSGKKLTGTR ADLVFGSHSE LRALAEVYGS ADGQQKFTKD 730 740 750 FVAAWDKVMN LDRFDVRRGI YDETRLKSKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of the rice blast fungus Magnaporthe grisea." Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K., Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D., Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S., Soanes D.M. , Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M., Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E., Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W. Nature 434:980-986(2005) [PubMed: 15846337] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 70-15 / FGSC 8958.
[2]	"Evolution of catalases from bacteria to humans." Zamocky M., Furtmueller P.G., Obinger C. Antioxid. Redox Signal. 10:1527-1548(2008) [PubMed: 18498226] [Abstract] Cited for: GENE NAME.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH476831 Genomic DNA. Translation: EDJ99644.1.
RefSeq	XP_361863.2.
3D structure databases
ModBase	Search...
Protein family/group databases
PeroxiBase	2288. MagCP01.
Genome annotation databases
GeneID	2677580.
KEGG	mgr:MGG_04337.
Phylogenomic databases
OMA	A4R5S9. FEWELTK.
Family and domain databases
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG1_MAGGR

Accession

Primary (citable) accession number: A4R5S9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	May 15, 2007
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)