A4QYQ5 (DAPB_MAGO7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable dipeptidyl-aminopeptidase B
Short name=DPAP B
EC=3.4.14.5

Gene names

Name:	DAPB
ORF Names:	MGG_07745

Organism

Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae) [Complete proteome]

Taxonomic identifier

242507 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Sordariomycetidae › Magnaporthales › Magnaporthaceae › Magnaporthe › Magnaporthe oryzae

Protein attributes

Sequence length	938 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Type IV dipeptidyl-peptidase which removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline By similarity.
Catalytic activity	Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.
Subcellular location	Vacuole membrane; Single-pass type II membrane protein By similarity. Note: Lysosome-like vacuoles By similarity.
Sequence similarities	Belongs to the peptidase S9B family.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Aminopeptidase Hydrolase Protease Serine protease
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 938	938	Probable dipeptidyl-aminopeptidase B		PRO_0000412147
Regions
	Topological domain	1 – 99	99	Cytoplasmic Potential
	Transmembrane	100 – 120	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	121 – 938	818	Vacuolar Potential
	Compositional bias	56 – 61	6	Poly-Asp
Sites
	Active site	765	1	Charge relay system By similarity
	Active site	842	1	Charge relay system By similarity
	Active site	875	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	220	1	N-linked (GlcNAc...) Potential
	Glycosylation	360	1	N-linked (GlcNAc...) Potential
	Glycosylation	824	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A4QYQ5 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 394BD6652B2C8283	FASTA	938	105,281
	10 20 30 40 50 60 MPVDKKSGNG RPSLDSVSSI STTSIVFDHL NDLEIHKKHR PHLMTESIRR DDHLSDFDDD 70 80 90 100 110 120 DPLKQEMDGE GDLETGPFLA GRDAYTFGGG KYMDRKFRRI MIVVAGLLVA AWVGGLFVYI 130 140 150 160 170 180 ASKSYKHATT VEHDPEKTKA AALTSGKKIT MDQVRSGTWY AASHDITWIA GPKGEDGLMI 190 200 210 220 230 240 EVGADGKDYI VIEDGRAENP DSSAEAEVDV TASRRTLMKN GSFRYQGRMH IPSGAEPSRD 250 260 270 280 290 300 QQKVLLRTEQ KSNWRHSHTA CYWIWDVATE TAEPLIPASP DARVQNAQWS PTSDAIVFTR 310 320 330 340 350 360 ENNLYLRTIG SKKVTQITKD GGADLFYGVP DWVYEEEVIA GASTTWWSED GKYVAFLRTN 370 380 390 400 410 420 ETGVPAFPVQ YFMSRPSGAS PKAGEETYPE VRDIKYPRSG SHNPVVDVQF YDIARGDVFT 430 440 450 460 470 480 VDINGGFADE DRLITTVLWA GSQVIVKETN RVSDIMRVVL IDVKKRTGKT TRTVDVGKID 490 500 510 520 530 540 GGWFEISQNT KYIPADPKKG RDQDGYIDTV IHNDGDHLAY FSPPDNPDPI MLTSGPGWEV 550 560 570 580 590 600 VDAPSAVDLE NNLVYFIATK EGSTQRHVYS VQLDGKNMKS FTDTEAKGYY DVSFSSGAGY 610 620 630 640 650 660 ALLSYKGPKI PWQKVVSTPA NNQRYEVLIE ENKELAESAR KYDLPLLNYG TLNVEGVELN 670 680 690 700 710 720 YLERRPPQFS EKKKYPVLFH QYSGPGSQSV SQKFSVDFQS YVAASLGYIV VTFDGRGTGF 730 740 750 760 770 780 IGRKNRVLVR SRLGEIEAQD QIAAAKHWAS LGYVDPSRIA IWGWSYGGFQ TLKTLEADAG 790 800 810 820 830 840 RTFSYGMAVA PVTDWRFYDS IYTERYMLTP QQNEDGYTKS AVHNVSALAS NKRFLLMHGA 850 860 870 880 890 900 SDDNVHFQNS LTLLDKLDMG AVENYDVHVF PDSDHSIFFH NANKIIYDKL ENWLVNAFNG 910 920 930 EWLKVNNAKP VELVHPTQLV REREKAKLRK RWQEIQNK

References

[1]

"The genome sequence of the rice blast fungus Magnaporthe grisea." Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K., Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D., Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S., Soanes D.M. , Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M., Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E., Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W. Nature 434:980-986(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 70-15 / ATCC MYA-4617 / FGSC 8958.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CM001233 Genomic DNA. Translation: EHA53149.1.
RefSeq	XP_003712956.1. XM_003712908.1.
3D structure databases
ProteinModelPortal	A4QYQ5.
ModBase	Search...
Protein family/group databases
MEROPS	S09.006.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	MGG_07745T0; MGG_07745T0; MGG_07745.
GeneID	2683672.
KEGG	mgr:MGG_07745.
Phylogenomic databases
KO	K01282.
OrthoDB	EOG4Q885J.
Family and domain databases
InterPro	IPR001375. Peptidase_S9. IPR002469. Peptidase_S9B. [Graphical view]
Pfam	PF00930. DPPIV_N. 1 hit. PF00326. Peptidase_S9. 1 hit. [Graphical view]
PROSITE	PS00708. PRO_ENDOPEP_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPB_MAGO7

Accession

Primary (citable) accession number: A4QYQ5
Secondary accession number(s): G4N0L8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	May 15, 2007
Last modified:	November 28, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program