Reviewed, UniProtKB/Swiss-Prot A4QUT2 (KATG2_MAGGR)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 2
      Short name=CP 2
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 2

Gene names

Name:	katG2
Synonyms:	MagKatG2
ORF Names:	MGG_09834

Organism

Magnaporthe grisea (Rice blast fungus) (Pyricularia grisea) [Complete proteome]

Taxonomic identifier

148305 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Magnaporthales › Magnaporthaceae › Magnaporthe

Protein attributes

Sequence length	786 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. Donor + H2O2 = oxidized donor + 2 H2O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Subcellular location	Secreted Potential.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Cellular component	Secreted
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	catalase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 786	763	Catalase-peroxidase 2		PRO_0000345091
Sites
	Active site	141	1	Proton acceptor By similarity
	Metal binding	314	1	Iron (heme axial ligand) By similarity
	Site	137	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	140 ↔ 273		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-299) By similarity
	Cross-link	273 ↔ 299		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-140) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4QUT2-1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 2A6C8B2B043C3879	FASTA	786	85,587
	10 20 30 40 50 60 MHASLSSWLL AASLLTQPIS VSGQGCPFAK RDGTVDSSLP QKRADAPETT TFGRCAVKSN 70 80 90 100 110 120 QAGGGTRSHD WWPCQLRLDV LRQFQPSQNP LGGDFDYAEA FQSLDYEAVK KDIAALMTES 130 140 150 160 170 180 QDWWPADFGN YGGLFVRMAW HSAGTYRAMD GRGGGGMGQQ RFAPLNSWPD NQNLDKARRL 190 200 210 220 230 240 IWPIKQKYGN KISWADLMLL TGNVALENMG FKTLGFGGGR ADTWQSDEAV YWGAETTFVP 250 260 270 280 290 300 QGNDVRYNNS VDINARADKL EKPLAATHMG LIYVNPEGPN GTPDPAASAK DIREAFGRMG 310 320 330 340 350 360 MNDTETVALI AGGHAFGKTH GAVKGSNIGP APEAADLGMQ GLGWHNSVGD GNGPNQMTSG 370 380 390 400 410 420 LEVIWTKTPT KWSNGYLESL INNNWTLVES PAGAHQWEAV NGTVDYPDPF DKTKFRKATM 430 440 450 460 470 480 LTSDLALIND PEYLKISQRW LEHPEELADA FAKAWFKLLH RDLGPTTRYL GPEVPKESFI 490 500 510 520 530 540 WQDPLPAREG DLIDDADVDK LKAAILSTDG LDVSKLASTA MACATTYRNS DKRGGCNGAR 550 560 570 580 590 600 IALEPQRNWV SNNPTQLSAV LDALKKVQSD FNGSNGNKKV SLADLIVLGG TAAVEKAAKD 610 620 630 640 650 660 AGVDIKVPFS AGRVDATQEQ TDVTQFSYLE PQADGFRNYG RGTARARTEE IMVDKASQLT 670 680 690 700 710 720 LTPPELTVLV GGMRALGANY DGSDVGVFTA NKGKLTPDFF VNLVDMNIAW TASGADGESW 730 740 750 760 770 780 VGTDRKSRSE KYKGSRADLV FGSHAELRAI AEVYAENGNQ EKFVKDFVAA WTKVMNLDRF DLKVKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of the rice blast fungus Magnaporthe grisea." Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K., Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D., Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S., Soanes D.M. , Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M., Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E., Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W. Nature 434:980-986(2005) [PubMed: 15846337] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 70-15 / FGSC 8958.
[2]	"Evolution of catalases from bacteria to humans." Zamocky M., Furtmueller P.G., Obinger C. Antioxid. Redox Signal. 10:1527-1548(2008) [PubMed: 18498226] [Abstract] Cited for: GENE NAME.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH476821 Genomic DNA. Translation: EDJ95794.1.
RefSeq	XP_364989.1.
UniGene	Mgr.13376
3D structure databases
ModBase	Search...
Protein family/group databases
PeroxiBase	2337. MagCP02.
Genome annotation databases
GeneID	2680833.
KEGG	mgr:MGG_09834.
NMPDR	fig|242507.1.peg.6728.
Family and domain databases
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG2_MAGGR

Accession

Primary (citable) accession number: A4QUT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	May 15, 2007
Last modified:	October 13, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)