Reviewed, UniProtKB/Swiss-Prot A4QUC1 (LKHA4_MAGGR)

Names and origin

Protein names

Recommended name:
    Leukotriene A-4 hydrolase
    EC=3.3.2.6
Alternative name(s):
    Leukotriene A(4) hydrolase
      Short name=LTA-4 hydrolase

Gene names

ORF Names:

MGG_09481

Organism

Magnaporthe grisea (Rice blast fungus) (Pyricularia grisea) [Complete proteome]

Taxonomic identifier

148305 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Magnaporthales › Magnaporthaceae › Magnaporthe

Protein attributes

Sequence length	613 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolyzes an epoxide moiety of LTA4 to form LTB4. The enzyme also has some peptidase activity By similarity.
Catalytic activity	(7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O = (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Lipid metabolism; leukotriene B4 biosynthesis.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase M1 family.

Ontologies

Keywords
Biological process	Leukotriene biosynthesis
Cellular component	Cytoplasm Nucleus
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	leukotriene biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inositol or phosphatidylinositol kinase activity Inferred from electronic annotation. Source: InterPro leukotriene-A4 hydrolase activity Inferred from electronic annotation. Source: EC metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 613	613	Leukotriene A-4 hydrolase		PRO_0000324932
Sites
	Active site	295	1	By similarity
	Active site	382	1	Proton donor By similarity
	Metal binding	294	1	Zinc; catalytic By similarity
	Metal binding	298	1	Zinc; catalytic By similarity
	Metal binding	317	1	Zinc; catalytic By similarity
	Site	198	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4QUC1-1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: A5DFF832C8519E0C	FASTA	613	69,141
	10 20 30 40 50 60 MAARDPSTAS NYDAWKTKHT TANLRIDFDD KCLRGSVVLE LESRTAKESK EIVLDSSYVS 70 80 90 100 110 120 VESIKLNDVQ TKWEIKERNG PMGSPLHISV PEGADSGEVV RLEMAVKTTP QCTALQWLTP 130 140 150 160 170 180 AQTSNKKAPF MFSQAQACHA RSLFPCQDTP DVKSTYSFNI TSPHVVVASG VANDGDKAEA 190 200 210 220 230 240 DGGDKVYKYE QNVPIPSYLF ALASGDIAMA PIGPRSSVAT GPDEVKECQW ELEEDMGKFM 250 260 270 280 290 300 DAAERLVFPY RWGEYNVLVL PPSFPYGGME NPIYTFATPT IISGDRQNTD VIAHELSHSW 310 320 330 340 350 360 SGNLVTSCSW EHYWLNEGWT MYLERRIIAA VRGPAYFDFS ALLGWKHLED AIEEFGADHK 370 380 390 400 410 420 FTQLCINHKG IDPDDAFSTV PYEKGFHMVY YLDRLVGRKN FDKFIPYYFT KWANKSLDSY 430 440 450 460 470 480 EFKDTFLEFF DKPEYADLKD KIAGIDWEGR FYTPGLPPKP EFDTSLVDVC YALADKWKKG 490 500 510 520 530 540 DYTPSSKDVD GWTGNQKLVF LGSVQDFEQP LSAEQAKQLG NAYDLIETKN VELKTAYYLI 550 560 570 580 590 600 ALRAQDSTAY QGVADLLGQV GRMKFVRPLF RALNKVDRPL ALTTFEKNKD FYHPICRAMA 610 EKDLGLSDDA KKE

References

[1]

"The genome sequence of the rice blast fungus Magnaporthe grisea." Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K., Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D., Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S., Soanes D.M. , Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M., Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E., Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W. Nature 434:980-986(2005) [PubMed: 15846337] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 70-15 / FGSC 8958.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH476820 Genomic DNA. Translation: EDJ95633.1.
RefSeq	XP_364636.1.
UniGene	Mgr.13026
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2680552.
KEGG	mgr:MGG_09481.
NMPDR	fig|242507.1.peg.6375.
Phylogenomic databases
OMA	GQEVKYT.
Enzyme and pathway databases
BRENDA	3.3.2.6. 5953.
Family and domain databases
InterPro	IPR006025. Pept_M_Zn_BS. IPR001930. Peptidase_M1. IPR015211. Peptidase_M1_C. IPR014782. Peptidase_M1_N. IPR001263. PI3Ka. [Graphical view]
Gene3D	G3DSA:1.25.40.70. PI3Ka. 1 hit.
PANTHER	PTHR11533. Peptidase_M1. 1 hit.
Pfam	PF09127. Leuk-A4-hydro_C. 1 hit. PF01433. Peptidase_M1. 1 hit. [Graphical view]
PRINTS	PR00756. ALADIPTASE.
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LKHA4_MAGGR

Accession

Primary (citable) accession number: A4QUC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	May 15, 2007
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)