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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkmb

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Unreviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (gpib), Glucose-6-phosphate isomerase (gpia), Glucose-6-phosphate isomerase (gpib), Glucose-6-phosphate isomerase (gpia), Glucose-6-phosphate isomerase (gpia), Glucose-6-phosphate isomerase (gpia), Glucose-6-phosphate isomerase (gpib)
  3. ATP-dependent 6-phosphofructokinase (pfkla), ATP-dependent 6-phosphofructokinase (pfkmb), ATP-dependent 6-phosphofructokinase (pfklb), ATP-dependent 6-phosphofructokinase (pfkpa), ATP-dependent 6-phosphofructokinase (pfkpb), ATP-dependent 6-phosphofructokinase (pfkmb), ATP-dependent 6-phosphofructokinase (pfkla), ATP-dependent 6-phosphofructokinase (pfkma), ATP-dependent 6-phosphofructokinase (pfkpb), ATP-dependent 6-phosphofructokinase (pfklb), ATP-dependent 6-phosphofructokinase (pfkpa)
  4. Fructose-bisphosphate aldolase C-A (aldoca), Fructose-bisphosphate aldolase (aldob), Fructose-bisphosphate aldolase (aldoaa), Fructose-bisphosphate aldolase (aldoca), Fructose-bisphosphate aldolase C-B (aldocb), Fructose-bisphosphate aldolase B (aldob), Fructose-bisphosphate aldolase (aldoab), Fructose-bisphosphate aldolase (aldoaa), Fructose-bisphosphate aldolase (aldob), Fructose-bisphosphate aldolase (aldocb)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei25ATP; via amide nitrogenUniRule annotation1
Metal bindingi119Magnesium; catalyticUniRule annotation1
Active sitei166Proton acceptorUniRule annotation1
Binding sitei201Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei264SubstrateUniRule annotation1
Binding sitei292Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei470Allosteric activator fructose 2,6-bisphosphateUniRule annotation1
Binding sitei565Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei628Allosteric activator fructose 2,6-bisphosphateUniRule annotation1
Binding sitei654Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei734Allosteric activator fructose 2,6-bisphosphateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi88 – 89ATPUniRule annotation2
Nucleotide bindingi118 – 121ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzymeUniRule annotation, KinaseUniRule annotation, Transferase
Biological processGlycolysisUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkmbImported
Synonyms:pfkmImported
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)Imported
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Organism-specific databases

ZFINiZDB-GENE-081114-1 pfkmb

Subcellular locationi

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Proteomic databases

PRIDEiA4QNV1

Interactioni

Subunit structurei

Homo- and heterotetramers.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA4QNV1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 323PFKInterPro annotationAdd BLAST306
Domaini401 – 686PFKInterPro annotationAdd BLAST286

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 390N-terminal catalytic PFK domain 1UniRule annotationAdd BLAST390
Regioni164 – 166Substrate bindingUniRule annotation3
Regioni208 – 210Substrate bindingUniRule annotation3
Regioni298 – 301Substrate bindingUniRule annotation4
Regioni401 – 776C-terminal regulatory PFK domain 2UniRule annotationAdd BLAST376
Regioni527 – 531Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation5
Regioni572 – 574Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation3
Regioni660 – 663Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation4

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili601 – 621Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

eggNOGiKOG2440 Eukaryota
COG0205 LUCA
HOGENOMiHOG000200154
HOVERGENiHBG000976
KOiK00850
PhylomeDBiA4QNV1

Family and domain databases

HAMAPiMF_03184 Phosphofructokinase_I_E, 1 hit
InterProiView protein in InterPro
IPR009161 6-Pfructokinase_euk
IPR022953 ATP_PFK
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 2 hits
PIRSFiPIRSF000533 ATP_PFK_euk, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 2 hits
TIGRFAMsiTIGR02478 6PF1K_euk, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 2 hits

Sequencei

Sequence statusi: Complete.

A4QNV1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQHSAPVDPT KMGVGRAIAV LTSGGDAQGM NAAVRATVRV GIYTGAKVYF
60 70 80 90 100
VHEGYQGLVD GGDNIRQATW ESVSMMLQLG GTVIGSARCQ DFRTKEGRAK
110 120 130 140 150
AACNLVKLGI TNLCVIGGDG SLTGANEFRN EWSELLQILQ KAGKITAEEA
160 170 180 190 200
KCSSHLNIVG MVGSIDNDFC GTDMTIGTDS ALHRIMEVVD AITTTAQSHQ
210 220 230 240 250
RAFILEVMGR HCGYLALVTA LSCGADWVFI PEMPPDEGWE DHLCRRLTYQ
260 270 280 290 300
RSIGNRLNVI IVAEGALDRH GKPITCDIIK NLVTKKLGFD TRATILGHVQ
310 320 330 340 350
RGGTPSAFDR ILGSRMGVEA VMALLEATPD TPACVVSLSG NMAVRLPLME
360 370 380 390 400
CVQVTKEVTK AMAEGRFEEA IKLRGKSFEN NWNTYKLLAH VTAPDVKSNI
410 420 430 440 450
NIAILNVGAP CAGMNAAVRS AVRIGILQGH NMLAVHDGFE GLANGTIEPM
460 470 480 490 500
AWGYVGGWTG KGGSILGTKR SLPANMIEEI SLNIAKFNIH ALVIIGGFEA
510 520 530 540 550
FVGGLELVTA REKYEELCIP LVVIPATVSN NVPGSDFSVG ADTALNTITT
560 570 580 590 600
TCDRIKQSAA GTKRRVFIIE TMGGYCGYLA TMAGLAAGAD AAYVFEDPFG
610 620 630 640 650
IHDLEANVEH LLEKMKTTVK RGLILRNEKC NANYTTDFLF NLYSEEGKGV
660 670 680 690 700
FDCRKNVLGH MQQGGTPTPF DRNFGTKMGA KAVLWLTDKL KECYRHGRIF
710 720 730 740 750
ANTPDSACVL GMRKRAMLFQ PLSDLKEDTD FEHRIPKTEW WLKLRPILKI
760 770
LAKYKISLDT SETATMEHVI KKRGGV
Length:776
Mass (Da):84,166
Last modified:May 15, 2007 - v1
Checksum:i92D93282047C995D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC139532 mRNA Translation: AAI39533.1
RefSeqiNP_001082886.1, NM_001089417.1
UniGeneiDr.41754

Genome annotation databases

GeneIDi568001
KEGGidre:568001

Similar proteinsi

Entry informationi

Entry nameiA4QNV1_DANRE
AccessioniPrimary (citable) accession number: A4QNV1
Entry historyiIntegrated into UniProtKB/TrEMBL: May 15, 2007
Last sequence update: May 15, 2007
Last modified: March 28, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)