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UniProtKB - A4QNV1 (A4QNV1_DANRE)

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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkmb

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (gpib), Glucose-6-phosphate isomerase (gpia), Glucose-6-phosphate isomerase (gpib), Glucose-6-phosphate isomerase (gpia), Glucose-6-phosphate isomerase (gpia), Glucose-6-phosphate isomerase (gpia), Glucose-6-phosphate isomerase (gpib)
  3. ATP-dependent 6-phosphofructokinase (pfkla), ATP-dependent 6-phosphofructokinase (pfkmb), ATP-dependent 6-phosphofructokinase (pfklb), ATP-dependent 6-phosphofructokinase (pfkpa), ATP-dependent 6-phosphofructokinase (pfkpb), ATP-dependent 6-phosphofructokinase (pfkmb), ATP-dependent 6-phosphofructokinase (pfkla), ATP-dependent 6-phosphofructokinase (pfkma), ATP-dependent 6-phosphofructokinase (pfklb), ATP-dependent 6-phosphofructokinase (pfkpb), ATP-dependent 6-phosphofructokinase (pfkpa)
  4. Fructose-bisphosphate aldolase C-A (aldoca), Fructose-bisphosphate aldolase (aldob), Fructose-bisphosphate aldolase (aldoaa), Fructose-bisphosphate aldolase (aldoca), Fructose-bisphosphate aldolase (aldoca), Fructose-bisphosphate aldolase C-B (aldocb), Fructose-bisphosphate aldolase B (aldob), Fructose-bisphosphate aldolase (aldoab), Fructose-bisphosphate aldolase (aldoaa), Fructose-bisphosphate aldolase (aldob), Fructose-bisphosphate aldolase (aldocb)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei25ATP; via amide nitrogenUniRule annotation1
Metal bindingi119Magnesium; catalyticUniRule annotation1
Active sitei166Proton acceptorUniRule annotation1
Binding sitei201Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei264SubstrateUniRule annotation1
Binding sitei292Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei470Allosteric activator fructose 2,6-bisphosphateUniRule annotation1
Binding sitei565Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei628Allosteric activator fructose 2,6-bisphosphateUniRule annotation1
Binding sitei654Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei734Allosteric activator fructose 2,6-bisphosphateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi88 – 89ATPUniRule annotation2
Nucleotide bindingi118 – 121ATPUniRule annotation4

GO - Molecular functioni

Complete GO annotation...

GO - Biological processi

Complete GO annotation...

Keywordsi

Molecular functionKinaseUniRule annotation, Transferase
Biological processGlycolysisUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkmbImported
Synonyms:pfkmImported
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)Imported
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Organism-specific databases

ZFINiZDB-GENE-081114-1. pfkmb.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Proteomic databases

PaxDbiA4QNV1.
PRIDEiA4QNV1.

Interactioni

Subunit structurei

Homo- and heterotetramers.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA4QNV1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 323PFKInterPro annotationAdd BLAST306
Domaini401 – 686PFKInterPro annotationAdd BLAST286

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 390N-terminal catalytic PFK domain 1UniRule annotationAdd BLAST390
Regioni164 – 166Substrate bindingUniRule annotation3
Regioni208 – 210Substrate bindingUniRule annotation3
Regioni298 – 301Substrate bindingUniRule annotation4
Regioni401 – 776C-terminal regulatory PFK domain 2UniRule annotationAdd BLAST376
Regioni527 – 531Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation5
Regioni572 – 574Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation3
Regioni660 – 663Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2440. Eukaryota.
COG0205. LUCA.
HOGENOMiHOG000200154.
HOVERGENiHBG000976.
KOiK00850.
PhylomeDBiA4QNV1.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E. 1 hit.
InterProiView protein in InterPro
IPR009161. 6-Pfructokinase_euk.
IPR022953. ATP_PFK.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
PfamiView protein in Pfam
PF00365. PFK. 2 hits.
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiView protein in PROSITE
PS00433. PHOSPHOFRUCTOKINASE. 2 hits.

Sequencei

Sequence statusi: Complete.

A4QNV1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQHSAPVDPT KMGVGRAIAV LTSGGDAQGM NAAVRATVRV GIYTGAKVYF 
        60         70         80         90        100
VHEGYQGLVD GGDNIRQATW ESVSMMLQLG GTVIGSARCQ DFRTKEGRAK 
       110        120        130        140        150
AACNLVKLGI TNLCVIGGDG SLTGANEFRN EWSELLQILQ KAGKITAEEA 
       160        170        180        190        200
KCSSHLNIVG MVGSIDNDFC GTDMTIGTDS ALHRIMEVVD AITTTAQSHQ 
       210        220        230        240        250
RAFILEVMGR HCGYLALVTA LSCGADWVFI PEMPPDEGWE DHLCRRLTYQ 
       260        270        280        290        300
RSIGNRLNVI IVAEGALDRH GKPITCDIIK NLVTKKLGFD TRATILGHVQ 
       310        320        330        340        350
RGGTPSAFDR ILGSRMGVEA VMALLEATPD TPACVVSLSG NMAVRLPLME 
       360        370        380        390        400
CVQVTKEVTK AMAEGRFEEA IKLRGKSFEN NWNTYKLLAH VTAPDVKSNI 
       410        420        430        440        450
NIAILNVGAP CAGMNAAVRS AVRIGILQGH NMLAVHDGFE GLANGTIEPM 
       460        470        480        490        500
AWGYVGGWTG KGGSILGTKR SLPANMIEEI SLNIAKFNIH ALVIIGGFEA 
       510        520        530        540        550
FVGGLELVTA REKYEELCIP LVVIPATVSN NVPGSDFSVG ADTALNTITT 
       560        570        580        590        600
TCDRIKQSAA GTKRRVFIIE TMGGYCGYLA TMAGLAAGAD AAYVFEDPFG 
       610        620        630        640        650
IHDLEANVEH LLEKMKTTVK RGLILRNEKC NANYTTDFLF NLYSEEGKGV 
       660        670        680        690        700
FDCRKNVLGH MQQGGTPTPF DRNFGTKMGA KAVLWLTDKL KECYRHGRIF 
       710        720        730        740        750
ANTPDSACVL GMRKRAMLFQ PLSDLKEDTD FEHRIPKTEW WLKLRPILKI 
       760        770 
LAKYKISLDT SETATMEHVI KKRGGV
Length:776
Mass (Da):84,166
Last modified:May 15, 2007 - v1
Checksum:i92D93282047C995D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC139532 mRNA. Translation: AAI39533.1.
RefSeqiNP_001082886.1. NM_001089417.1.
UniGeneiDr.41754.

Genome annotation databases

GeneIDi568001.
KEGGidre:568001.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiA4QNV1_DANRE
AccessioniPrimary (citable) accession number: A4QNV1
Entry historyiIntegrated into UniProtKB/TrEMBL: May 15, 2007
Last sequence update: May 15, 2007
Last modified: July 5, 2017
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Allosteric enzymeUniRule annotation