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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkmb

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei25 – 251ATP; via amide nitrogenUniRule annotation
Metal bindingi119 – 1191Magnesium; catalyticUniRule annotation
Active sitei166 – 1661Proton acceptorUniRule annotation
Binding sitei201 – 2011Substrate; shared with dimeric partnerUniRule annotation
Binding sitei264 – 2641SubstrateUniRule annotation
Binding sitei292 – 2921Substrate; shared with dimeric partnerUniRule annotation
Binding sitei470 – 4701Allosteric activator fructose 2,6-bisphosphateUniRule annotation
Binding sitei565 – 5651Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
Binding sitei628 – 6281Allosteric activator fructose 2,6-bisphosphateUniRule annotation
Binding sitei654 – 6541Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
Binding sitei734 – 7341Allosteric activator fructose 2,6-bisphosphateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi88 – 892ATPUniRule annotation
Nucleotide bindingi118 – 1214ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkmbImported
Synonyms:pfkmImported
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)Imported
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Organism-specific databases

ZFINiZDB-GENE-081114-1. pfkmb.

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homo- and heterotetramers.UniRule annotation

Protein-protein interaction databases

STRINGi7955.ENSDARP00000079712.

Structurei

3D structure databases

ProteinModelPortaliA4QNV1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 390390N-terminal catalytic PFK domain 1UniRule annotationAdd
BLAST
Regioni164 – 1663Substrate bindingUniRule annotation
Regioni208 – 2103Substrate bindingUniRule annotation
Regioni298 – 3014Substrate bindingUniRule annotation
Regioni391 – 40111Interdomain linkerUniRule annotationAdd
BLAST
Regioni401 – 776376C-terminal regulatory PFK domain 2UniRule annotationAdd
BLAST
Regioni527 – 5315Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni572 – 5743Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni660 – 6634Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000200154.
HOVERGENiHBG000976.
KOiK00850.
PhylomeDBiA4QNV1.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-Pfructokinase_euk.
IPR022953. ATP_PFK.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4QNV1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQHSAPVDPT KMGVGRAIAV LTSGGDAQGM NAAVRATVRV GIYTGAKVYF
60 70 80 90 100
VHEGYQGLVD GGDNIRQATW ESVSMMLQLG GTVIGSARCQ DFRTKEGRAK
110 120 130 140 150
AACNLVKLGI TNLCVIGGDG SLTGANEFRN EWSELLQILQ KAGKITAEEA
160 170 180 190 200
KCSSHLNIVG MVGSIDNDFC GTDMTIGTDS ALHRIMEVVD AITTTAQSHQ
210 220 230 240 250
RAFILEVMGR HCGYLALVTA LSCGADWVFI PEMPPDEGWE DHLCRRLTYQ
260 270 280 290 300
RSIGNRLNVI IVAEGALDRH GKPITCDIIK NLVTKKLGFD TRATILGHVQ
310 320 330 340 350
RGGTPSAFDR ILGSRMGVEA VMALLEATPD TPACVVSLSG NMAVRLPLME
360 370 380 390 400
CVQVTKEVTK AMAEGRFEEA IKLRGKSFEN NWNTYKLLAH VTAPDVKSNI
410 420 430 440 450
NIAILNVGAP CAGMNAAVRS AVRIGILQGH NMLAVHDGFE GLANGTIEPM
460 470 480 490 500
AWGYVGGWTG KGGSILGTKR SLPANMIEEI SLNIAKFNIH ALVIIGGFEA
510 520 530 540 550
FVGGLELVTA REKYEELCIP LVVIPATVSN NVPGSDFSVG ADTALNTITT
560 570 580 590 600
TCDRIKQSAA GTKRRVFIIE TMGGYCGYLA TMAGLAAGAD AAYVFEDPFG
610 620 630 640 650
IHDLEANVEH LLEKMKTTVK RGLILRNEKC NANYTTDFLF NLYSEEGKGV
660 670 680 690 700
FDCRKNVLGH MQQGGTPTPF DRNFGTKMGA KAVLWLTDKL KECYRHGRIF
710 720 730 740 750
ANTPDSACVL GMRKRAMLFQ PLSDLKEDTD FEHRIPKTEW WLKLRPILKI
760 770
LAKYKISLDT SETATMEHVI KKRGGV
Length:776
Mass (Da):84,166
Last modified:May 15, 2007 - v1
Checksum:i92D93282047C995D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC139532 mRNA. Translation: AAI39533.1.
RefSeqiNP_001082886.1. NM_001089417.1.
UniGeneiDr.41754.

Genome annotation databases

GeneIDi568001.
KEGGidre:568001.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC139532 mRNA. Translation: AAI39533.1.
RefSeqiNP_001082886.1. NM_001089417.1.
UniGeneiDr.41754.

3D structure databases

ProteinModelPortaliA4QNV1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000079712.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi568001.
KEGGidre:568001.

Organism-specific databases

CTDi568001.
ZFINiZDB-GENE-081114-1. pfkmb.

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000200154.
HOVERGENiHBG000976.
KOiK00850.
PhylomeDBiA4QNV1.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Miscellaneous databases

NextBioi20888958.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-Pfructokinase_euk.
IPR022953. ATP_PFK.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Whole bodyImported.

Entry informationi

Entry nameiA4QNV1_DANRE
AccessioniPrimary (citable) accession number: A4QNV1
Entry historyi
Integrated into UniProtKB/TrEMBL: May 15, 2007
Last sequence update: May 15, 2007
Last modified: April 29, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.