UniProtKB - A4QNV1 (A4QNV1_DANRE)
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Protein
ATP-dependent 6-phosphofructokinase
Gene
pfkmb
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Functioni
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation
Caution
Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
Catalytic activityi
ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation
Cofactori
Mg2+UniRule annotation
Enzyme regulationi
Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation
: glycolysis Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotationProteins known to be involved in the 4 steps of the subpathway in this organism are:
- no protein annotated in this organism
- Glucose-6-phosphate isomerase (gpib), Glucose-6-phosphate isomerase (gpia), Glucose-6-phosphate isomerase (gpib), Glucose-6-phosphate isomerase (gpia), Glucose-6-phosphate isomerase (gpia), Glucose-6-phosphate isomerase (gpia), Glucose-6-phosphate isomerase (gpib)
- ATP-dependent 6-phosphofructokinase (pfkla), ATP-dependent 6-phosphofructokinase (pfkmb), ATP-dependent 6-phosphofructokinase (pfklb), ATP-dependent 6-phosphofructokinase (pfkpa), ATP-dependent 6-phosphofructokinase (pfkpb), ATP-dependent 6-phosphofructokinase (pfkmb), ATP-dependent 6-phosphofructokinase (pfkla), ATP-dependent 6-phosphofructokinase (pfkma), ATP-dependent 6-phosphofructokinase (pfkpb), ATP-dependent 6-phosphofructokinase (pfklb), ATP-dependent 6-phosphofructokinase (pfkpa)
- Fructose-bisphosphate aldolase C-A (aldoca), Fructose-bisphosphate aldolase (aldob), Fructose-bisphosphate aldolase (aldoaa), Fructose-bisphosphate aldolase (aldoca), Fructose-bisphosphate aldolase C-B (aldocb), Fructose-bisphosphate aldolase B (aldob), Fructose-bisphosphate aldolase (aldoab), Fructose-bisphosphate aldolase (aldoaa), Fructose-bisphosphate aldolase (aldob), Fructose-bisphosphate aldolase (aldocb)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 25 | ATP; via amide nitrogenUniRule annotation | 1 | |
Metal bindingi | 119 | Magnesium; catalyticUniRule annotation | 1 | |
Active sitei | 166 | Proton acceptorUniRule annotation | 1 | |
Binding sitei | 201 | Substrate; shared with dimeric partnerUniRule annotation | 1 | |
Binding sitei | 264 | SubstrateUniRule annotation | 1 | |
Binding sitei | 292 | Substrate; shared with dimeric partnerUniRule annotation | 1 | |
Binding sitei | 470 | Allosteric activator fructose 2,6-bisphosphateUniRule annotation | 1 | |
Binding sitei | 565 | Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation | 1 | |
Binding sitei | 628 | Allosteric activator fructose 2,6-bisphosphateUniRule annotation | 1 | |
Binding sitei | 654 | Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation | 1 | |
Binding sitei | 734 | Allosteric activator fructose 2,6-bisphosphateUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 88 – 89 | ATPUniRule annotation | 2 | |
Nucleotide bindingi | 118 – 121 | ATPUniRule annotation | 4 |
GO - Molecular functioni
- 6-phosphofructokinase activity Source: UniProtKB-UniRule
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- fructose 6-phosphate metabolic process Source: InterPro
Keywordsi
Molecular function | Allosteric enzymeUniRule annotation, KinaseUniRule annotation, Transferase |
Biological process | GlycolysisUniRule annotation |
Ligand | ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding |
Enzyme and pathway databases
UniPathwayi | UPA00109; UER00182 |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)Short name: ATP-PFKUniRule annotation Short name: PhosphofructokinaseUniRule annotation Alternative name(s): PhosphohexokinaseUniRule annotation |
Gene namesi | |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio)Imported |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio |
Organism-specific databases
ZFINi | ZDB-GENE-081114-1 pfkmb |
Interactioni
Subunit structurei
Homo- and heterotetramers.UniRule annotation
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 18 – 323 | PFKInterPro annotationAdd BLAST | 306 | |
Domaini | 401 – 686 | PFKInterPro annotationAdd BLAST | 286 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 390 | N-terminal catalytic PFK domain 1UniRule annotationAdd BLAST | 390 | |
Regioni | 164 – 166 | Substrate bindingUniRule annotation | 3 | |
Regioni | 208 – 210 | Substrate bindingUniRule annotation | 3 | |
Regioni | 298 – 301 | Substrate bindingUniRule annotation | 4 | |
Regioni | 401 – 776 | C-terminal regulatory PFK domain 2UniRule annotationAdd BLAST | 376 | |
Regioni | 527 – 531 | Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation | 5 | |
Regioni | 572 – 574 | Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation | 3 | |
Regioni | 660 – 663 | Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation | 4 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 601 – 621 | Sequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation
Keywords - Domaini
Coiled coilSequence analysisPhylogenomic databases
eggNOGi | KOG2440 Eukaryota COG0205 LUCA |
HOGENOMi | HOG000200154 |
HOVERGENi | HBG000976 |
KOi | K00850 |
PhylomeDBi | A4QNV1 |
Family and domain databases
HAMAPi | MF_03184 Phosphofructokinase_I_E, 1 hit |
InterProi | View protein in InterPro IPR009161 6-Pfructokinase_euk IPR022953 ATP_PFK IPR015912 Phosphofructokinase_CS IPR000023 Phosphofructokinase_dom IPR035966 PKF_sf |
Pfami | View protein in Pfam PF00365 PFK, 2 hits |
PIRSFi | PIRSF000533 ATP_PFK_euk, 1 hit |
PRINTSi | PR00476 PHFRCTKINASE |
SUPFAMi | SSF53784 SSF53784, 2 hits |
TIGRFAMsi | TIGR02478 6PF1K_euk, 1 hit |
PROSITEi | View protein in PROSITE PS00433 PHOSPHOFRUCTOKINASE, 2 hits |
i Sequence
Sequence statusi: Complete.
A4QNV1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQHSAPVDPT KMGVGRAIAV LTSGGDAQGM NAAVRATVRV GIYTGAKVYF
60 70 80 90 100
VHEGYQGLVD GGDNIRQATW ESVSMMLQLG GTVIGSARCQ DFRTKEGRAK
110 120 130 140 150
AACNLVKLGI TNLCVIGGDG SLTGANEFRN EWSELLQILQ KAGKITAEEA
160 170 180 190 200
KCSSHLNIVG MVGSIDNDFC GTDMTIGTDS ALHRIMEVVD AITTTAQSHQ
210 220 230 240 250
RAFILEVMGR HCGYLALVTA LSCGADWVFI PEMPPDEGWE DHLCRRLTYQ
260 270 280 290 300
RSIGNRLNVI IVAEGALDRH GKPITCDIIK NLVTKKLGFD TRATILGHVQ
310 320 330 340 350
RGGTPSAFDR ILGSRMGVEA VMALLEATPD TPACVVSLSG NMAVRLPLME
360 370 380 390 400
CVQVTKEVTK AMAEGRFEEA IKLRGKSFEN NWNTYKLLAH VTAPDVKSNI
410 420 430 440 450
NIAILNVGAP CAGMNAAVRS AVRIGILQGH NMLAVHDGFE GLANGTIEPM
460 470 480 490 500
AWGYVGGWTG KGGSILGTKR SLPANMIEEI SLNIAKFNIH ALVIIGGFEA
510 520 530 540 550
FVGGLELVTA REKYEELCIP LVVIPATVSN NVPGSDFSVG ADTALNTITT
560 570 580 590 600
TCDRIKQSAA GTKRRVFIIE TMGGYCGYLA TMAGLAAGAD AAYVFEDPFG
610 620 630 640 650
IHDLEANVEH LLEKMKTTVK RGLILRNEKC NANYTTDFLF NLYSEEGKGV
660 670 680 690 700
FDCRKNVLGH MQQGGTPTPF DRNFGTKMGA KAVLWLTDKL KECYRHGRIF
710 720 730 740 750
ANTPDSACVL GMRKRAMLFQ PLSDLKEDTD FEHRIPKTEW WLKLRPILKI
760 770
LAKYKISLDT SETATMEHVI KKRGGV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC139532 mRNA Translation: AAI39533.1 |
RefSeqi | NP_001082886.1, NM_001089417.1 |
UniGenei | Dr.41754 |
Genome annotation databases
GeneIDi | 568001 |
KEGGi | dre:568001 |
Similar proteinsi
Entry informationi
Entry namei | A4QNV1_DANRE | |
Accessioni | A4QNV1Primary (citable) accession number: A4QNV1 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | May 15, 2007 |
Last sequence update: | May 15, 2007 | |
Last modified: | March 28, 2018 | |
This is version 90 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |