pfkmb

Functionⁱ

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityⁱ

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactorⁱ

Mg²⁺UniRule annotation

Enzyme regulationⁱ

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

Pathwayⁱ: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:

This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	25	ATP; via amide nitrogenUniRule annotation	1
Metal bindingⁱ	119	Magnesium; catalyticUniRule annotation	1
Active siteⁱ	166	Proton acceptorUniRule annotation	1
Binding siteⁱ	201	Substrate; shared with dimeric partnerUniRule annotation	1
Binding siteⁱ	264	SubstrateUniRule annotation	1
Binding siteⁱ	292	Substrate; shared with dimeric partnerUniRule annotation	1
Binding siteⁱ	470	Allosteric activator fructose 2,6-bisphosphateUniRule annotation	1
Binding siteⁱ	565	Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation	1
Binding siteⁱ	628	Allosteric activator fructose 2,6-bisphosphateUniRule annotation	1
Binding siteⁱ	654	Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation	1
Binding siteⁱ	734	Allosteric activator fructose 2,6-bisphosphateUniRule annotation	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	88 – 89	ATPUniRule annotation		2
Nucleotide bindingⁱ	118 – 121	ATPUniRule annotation		4

GO - Molecular functionⁱ

6-phosphofructokinase activity Source: UniProtKB-UniRule
ATP binding Source: UniProtKB-KW
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

fructose 6-phosphate metabolic process Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Allosteric enzymeUniRule annotation, KinaseUniRule annotation, Transferase
Biological process	GlycolysisUniRule annotation
Ligand	ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayⁱ	UPA00109; UER00182

UniPathwayⁱ

UPA00109; UER00182

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation) Short name: ATP-PFKUniRule annotation Short name: PhosphofructokinaseUniRule annotation Alternative name(s): PhosphohexokinaseUniRule annotation
Gene namesⁱ	Name:pfkmbImported Synonyms:pfkmImported
Organismⁱ	Danio rerio (Zebrafish) (Brachydanio rerio)Imported
Taxonomic identifierⁱ	7955 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Osteoglossocephalai › Clupeocephala › Otomorpha › Ostariophysi › Otophysi › Cypriniphysae › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Organism-specific databases

ZFINⁱ	ZDB-GENE-081114-1 pfkmb

ZFINⁱ

ZDB-GENE-081114-1 pfkmb

Keywords - Cellular componentⁱ

CytoplasmUniRule annotation

PTM / Processingⁱ

Proteomic databases

PRIDEⁱ	A4QNV1

PRIDEⁱ

A4QNV1

Interactionⁱ

Subunit structureⁱ

Homo- and heterotetramers.UniRule annotation

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	A4QNV1
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	18 – 323	PFKInterPro annotationAdd BLAST		306
Domainⁱ	401 – 686	PFKInterPro annotationAdd BLAST		286

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	1 – 390	N-terminal catalytic PFK domain 1UniRule annotationAdd BLAST	390
Regionⁱ	164 – 166	Substrate bindingUniRule annotation	3
Regionⁱ	208 – 210	Substrate bindingUniRule annotation	3
Regionⁱ	298 – 301	Substrate bindingUniRule annotation	4
Regionⁱ	401 – 776	C-terminal regulatory PFK domain 2UniRule annotationAdd BLAST	376
Regionⁱ	527 – 531	Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation	5
Regionⁱ	572 – 574	Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation	3
Regionⁱ	660 – 663	Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation	4

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	601 – 621	Sequence analysisAdd BLAST		21

Sequence similaritiesⁱ

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

Keywords - Domainⁱ

Coiled coilSequence analysis

Phylogenomic databases

eggNOGⁱ	KOG2440 Eukaryota COG0205 LUCA
HOGENOMⁱ	HOG000200154
HOVERGENⁱ	HBG000976
KEGG Orthology (KO) More...KOⁱ	K00850
PhylomeDBⁱ	A4QNV1

Family and domain databases

HAMAPⁱ	MF_03184 Phosphofructokinase_I_E, 1 hit
InterProⁱ	View protein in InterPro IPR009161 6-Pfructokinase_euk IPR022953 ATP_PFK IPR015912 Phosphofructokinase_CS IPR000023 Phosphofructokinase_dom IPR035966 PKF_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00365 PFK, 2 hits
PIRSFⁱ	PIRSF000533 ATP_PFK_euk, 1 hit
PRINTSⁱ	PR00476 PHFRCTKINASE
SUPFAMⁱ	SSF53784 SSF53784, 2 hits
TIGRFAMsⁱ	TIGR02478 6PF1K_euk, 1 hit
PROSITEⁱ	View protein in PROSITE PS00433 PHOSPHOFRUCTOKINASE, 2 hits

Sequenceⁱ

Sequence statusⁱ: Complete.

A4QNV1-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MQHSAPVDPT KMGVGRAIAV LTSGGDAQGM NAAVRATVRV GIYTGAKVYF 
        60         70         80         90        100
VHEGYQGLVD GGDNIRQATW ESVSMMLQLG GTVIGSARCQ DFRTKEGRAK 
       110        120        130        140        150
AACNLVKLGI TNLCVIGGDG SLTGANEFRN EWSELLQILQ KAGKITAEEA 
       160        170        180        190        200
KCSSHLNIVG MVGSIDNDFC GTDMTIGTDS ALHRIMEVVD AITTTAQSHQ 
       210        220        230        240        250
RAFILEVMGR HCGYLALVTA LSCGADWVFI PEMPPDEGWE DHLCRRLTYQ 
       260        270        280        290        300
RSIGNRLNVI IVAEGALDRH GKPITCDIIK NLVTKKLGFD TRATILGHVQ 
       310        320        330        340        350
RGGTPSAFDR ILGSRMGVEA VMALLEATPD TPACVVSLSG NMAVRLPLME 
       360        370        380        390        400
CVQVTKEVTK AMAEGRFEEA IKLRGKSFEN NWNTYKLLAH VTAPDVKSNI 
       410        420        430        440        450
NIAILNVGAP CAGMNAAVRS AVRIGILQGH NMLAVHDGFE GLANGTIEPM 
       460        470        480        490        500
AWGYVGGWTG KGGSILGTKR SLPANMIEEI SLNIAKFNIH ALVIIGGFEA 
       510        520        530        540        550
FVGGLELVTA REKYEELCIP LVVIPATVSN NVPGSDFSVG ADTALNTITT 
       560        570        580        590        600
TCDRIKQSAA GTKRRVFIIE TMGGYCGYLA TMAGLAAGAD AAYVFEDPFG 
       610        620        630        640        650
IHDLEANVEH LLEKMKTTVK RGLILRNEKC NANYTTDFLF NLYSEEGKGV 
       660        670        680        690        700
FDCRKNVLGH MQQGGTPTPF DRNFGTKMGA KAVLWLTDKL KECYRHGRIF 
       710        720        730        740        750
ANTPDSACVL GMRKRAMLFQ PLSDLKEDTD FEHRIPKTEW WLKLRPILKI 
       760        770 
LAKYKISLDT SETATMEHVI KKRGGV

Length:776

Mass (Da):84,166

Last modified:May 15, 2007 - v1

Checksum:ⁱ92D93282047C995D

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BC139532 mRNA Translation: AAI39533.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001082886.1, NM_001089417.1
UniGeneⁱ	Dr.41754

Genome annotation databases

GeneIDⁱ	568001
KEGGⁱ	dre:568001

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
A4QNV1	UPI0007B86C44 UPI0007AC9402 UPI0007B7AF5D UPI0007B86D52 UPI0007ACDEC7 UPI0007B9303E F1R047 UPI0001E9204B UPI0007BAAA20 UPI0007ACF2FB +1	Sinocyclocheilus anshuiensis Sinocyclocheilus grahami (Dianchi golden-line fish) (Barbus grahami) Sinocyclocheilus rhinocerous Danio rerio (Zebrafish) (Brachydanio rerio) Astyanax mexicanus (Blind cave fish) (Astyanax fasciatus mexicanus)	776	UniRef90_A4QNV1	Cluster: ATP-dependent 6-phosphofructokinase	12

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
A4QNV1	P17858 K7DD35 A0A2J8M8K4 UPI0003C12DA1 L8Y245 UPI000A347F50 UPI000846C6BF UPI00057A91AF UPI000BB4CF11 UPI0001DEBFDC +379	Homo sapiens (Human) Pan troglodytes (Chimpanzee) Pantholops hodgsonii (Chiru) (Tibetan antelope) Tupaia chinensis (Chinese tree shrew) Heterocephalus glaber (Naked mole rat) Capra hircus (Goat) Camelus bactrianus (Bactrian camel) Enhydra lutris kenyoni Ailuropoda melanoleuca (Giant panda) Lipotes vexillifer (Yangtze river dolphin) And more	780	UniRef50_P17858	Cluster: ATP-dependent 6-phosphofructokinase, liver type	390

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BC139532 mRNA Translation: AAI39533.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001082886.1, NM_001089417.1
UniGeneⁱ	Dr.41754

3D structure databases

ProteinModelPortalⁱ	A4QNV1
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Proteomic databases

PRIDEⁱ	A4QNV1

PRIDEⁱ

A4QNV1

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	568001
KEGGⁱ	dre:568001

Organism-specific databases

CTDⁱ	568001
ZFINⁱ	ZDB-GENE-081114-1 pfkmb

Phylogenomic databases

eggNOGⁱ	KOG2440 Eukaryota COG0205 LUCA
HOGENOMⁱ	HOG000200154
HOVERGENⁱ	HBG000976
KEGG Orthology (KO) More...KOⁱ	K00850
PhylomeDBⁱ	A4QNV1

Enzyme and pathway databases

UniPathwayⁱ	UPA00109; UER00182

UniPathwayⁱ

UPA00109; UER00182

Family and domain databases

HAMAPⁱ	MF_03184 Phosphofructokinase_I_E, 1 hit
InterProⁱ	View protein in InterPro IPR009161 6-Pfructokinase_euk IPR022953 ATP_PFK IPR015912 Phosphofructokinase_CS IPR000023 Phosphofructokinase_dom IPR035966 PKF_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00365 PFK, 2 hits
PIRSFⁱ	PIRSF000533 ATP_PFK_euk, 1 hit
PRINTSⁱ	PR00476 PHFRCTKINASE
SUPFAMⁱ	SSF53784 SSF53784, 2 hits
TIGRFAMsⁱ	TIGR02478 6PF1K_euk, 1 hit
PROSITEⁱ	View protein in PROSITE PS00433 PHOSPHOFRUCTOKINASE, 2 hits
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	A4QNV1_DANRE
Accessionⁱ	A4QNV1Primary (citable) accession number: A4QNV1
Entry historyⁱ	Integrated into UniProtKB/TrEMBL:	May 15, 2007
	Last sequence update:	May 15, 2007
	Last modified:	March 28, 2018
	This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Unreviewed (UniProtKB/TrEMBL)

UniProtKB

UniParc

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UniRef

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Annotation systems

Supporting data

UniProtKB - A4QNV1 (A4QNV1_DANRE)

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ATP-dependent 6-phosphofructokinase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Caution

<p>This subsection of the ‘Function’ section describes an enzyme regulatory mechanism and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/enzyme_regulation' target='_top'>More...</a></p>Enzyme regulationi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: glycolysis

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Other locations

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Coiled coil

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

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Functionⁱ

Enzyme regulationⁱ

Pathwayⁱ: glycolysis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ