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Protein

Transcription factor IIIB 50 kDa subunit

Gene

brf2

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. Contributes to the regulation of gene expression; functions as activator in the absence of oxidative stress. Down-regulates expression of target genes in response to oxidative stress. Overexpression protects cells against apoptosis in response to oxidative stress.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7ZincBy similarity1
Metal bindingi10ZincBy similarity1
Metal bindingi28ZincBy similarity1
Metal bindingi31ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri3 – 36TFIIB-typePROSITE-ProRule annotationAdd BLAST34

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor IIIB 50 kDa subunit
Alternative name(s):
B-related factor 2
Short name:
BRF-2
Gene namesi
Name:brf2
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
Proteomesi
  • UP000008143 Componenti: Unassembled WGS sequence

Organism-specific databases

XenbaseiXB-GENE-964408 brf2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003371931 – 519Transcription factor IIIB 50 kDa subunitAdd BLAST519

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei462Cysteine sulfenic acid (-SOH)By similarity1

Post-translational modificationi

In response to oxidative stress, a Cys-residue is reversibly oxidized to cysteine sulfenic acid. This impairs formation of a ternary complex with TBP and DNA and down-regulates expression of target genes in response to oxidative stress.By similarity

Keywords - PTMi

Oxidation

Proteomic databases

PaxDbiA4QNR3

Interactioni

Subunit structurei

Component of TFIIIB complexes. Interacts with TBP and forms a ternary complex with TBp and target DNA sequences.By similarity

Protein-protein interaction databases

STRINGi8364.ENSXETP00000060533

Structurei

3D structure databases

ProteinModelPortaliA4QNR3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati173 – 2492Add BLAST77

Sequence similaritiesi

Belongs to the TFIIB family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri3 – 36TFIIB-typePROSITE-ProRule annotationAdd BLAST34

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1598 Eukaryota
COG1405 LUCA
HOVERGENiHBG107537
InParanoidiA4QNR3
KOiK15197

Family and domain databases

InterProiView protein in InterPro
IPR036915 Cyclin-like_sf
IPR000812 TFIIB
IPR013137 Znf_TFIIB
PANTHERiPTHR11618 PTHR11618, 2 hits
SUPFAMiSSF47954 SSF47954, 1 hit
PROSITEiView protein in PROSITE
PS51134 ZF_TFIIB, 1 hit

Sequencei

Sequence statusi: Complete.

A4QNR3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGAKQCPDC GSSDIVEDAH YSQDQVVCAD CGCILSEGLI TTTAAEESHL
60 70 80 90 100
QAVRFADSTG ENDSMTVSKL RGIVRVRNIC RVLRLPDGFS DTAVSYYEQA
110 120 130 140 150
YKHPLYHSVS IEKKEIIVGC CVYITCRQHQ WPITMATICS LVYAKKELFA
160 170 180 190 200
SIFLSIVQVL KLDVPSVSLQ NLVMSHCRSF KLFKDSCEVP SHYAEKLDTV
210 220 230 240 250
SERTVQTVEL AYETWLVTGR HPIPIITAAA YISWQSLLPA RRLSCSLSRF
260 270 280 290 300
CKLSDVDLPP PSAIRLRELQ GTLIKLSVYL PWLKVLSLNK KTVVQHLGDL
310 320 330 340 350
LRHRVFLLRK ALAVTEAELS RGTLADTEAQ LSRGTLADTE AQLSRGTLAD
360 370 380 390 400
TEAQLSRGTL ADTEAQLSRG TLADTEAQLS RGTLADTEAQ LSRGTLADTE
410 420 430 440 450
AQLSRGTLAD TEAQLSRGTL ADTVAQLSRG TLADTEAQLS RGTKALSSND
460 470 480 490 500
QPNSTFVFLP PCVSNPRKRS RSIPFPRGHL DITGDEDISD SEIEQYLRTP
510
AEMKEFEQAL NRDDELPNA
Length:519
Mass (Da):57,126
Last modified:May 15, 2007 - v1
Checksum:i55564CD7F1E36AF6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC136234 mRNA Translation: AAI36235.1
RefSeqiNP_001096549.1, NM_001103079.1
UniGeneiStr.53387

Genome annotation databases

GeneIDi100125194
KEGGixtr:100125194

Similar proteinsi

Entry informationi

Entry nameiBRF2_XENTR
AccessioniPrimary (citable) accession number: A4QNR3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 15, 2007
Last modified: March 28, 2018
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health