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Protein

Transcription factor IIIB 50 kDa subunit

Gene

brf2

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites (By similarity).By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi7 – 71ZincBy similarity
Metal bindingi10 – 101ZincBy similarity
Metal bindingi28 – 281ZincBy similarity
Metal bindingi31 – 311ZincBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3 – 3634TFIIB-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. zinc ion binding Source: InterPro

GO - Biological processi

  1. DNA-templated transcription, initiation Source: InterPro
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor IIIB 50 kDa subunit
Alternative name(s):
B-related factor 2
Short name:
BRF-2
Gene namesi
Name:brf2
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143 Componenti: Unplaced

Organism-specific databases

XenbaseiXB-GENE-964408. brf2.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 519519Transcription factor IIIB 50 kDa subunitPRO_0000337193Add
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati173 – 249772Add
BLAST

Sequence similaritiesi

Belongs to the TFIIB family.Curated
Contains 1 TFIIB-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3 – 3634TFIIB-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOVERGENiHBG107537.
InParanoidiA4QNR3.
KOiK15197.

Family and domain databases

Gene3Di1.10.472.10. 1 hit.
InterProiIPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERiPTHR11618. PTHR11618. 1 hit.
PfamiPF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 1 hit.
PROSITEiPS51134. ZF_TFIIB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4QNR3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGAKQCPDC GSSDIVEDAH YSQDQVVCAD CGCILSEGLI TTTAAEESHL
60 70 80 90 100
QAVRFADSTG ENDSMTVSKL RGIVRVRNIC RVLRLPDGFS DTAVSYYEQA
110 120 130 140 150
YKHPLYHSVS IEKKEIIVGC CVYITCRQHQ WPITMATICS LVYAKKELFA
160 170 180 190 200
SIFLSIVQVL KLDVPSVSLQ NLVMSHCRSF KLFKDSCEVP SHYAEKLDTV
210 220 230 240 250
SERTVQTVEL AYETWLVTGR HPIPIITAAA YISWQSLLPA RRLSCSLSRF
260 270 280 290 300
CKLSDVDLPP PSAIRLRELQ GTLIKLSVYL PWLKVLSLNK KTVVQHLGDL
310 320 330 340 350
LRHRVFLLRK ALAVTEAELS RGTLADTEAQ LSRGTLADTE AQLSRGTLAD
360 370 380 390 400
TEAQLSRGTL ADTEAQLSRG TLADTEAQLS RGTLADTEAQ LSRGTLADTE
410 420 430 440 450
AQLSRGTLAD TEAQLSRGTL ADTVAQLSRG TLADTEAQLS RGTKALSSND
460 470 480 490 500
QPNSTFVFLP PCVSNPRKRS RSIPFPRGHL DITGDEDISD SEIEQYLRTP
510
AEMKEFEQAL NRDDELPNA
Length:519
Mass (Da):57,126
Last modified:May 14, 2007 - v1
Checksum:i55564CD7F1E36AF6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC136234 mRNA. Translation: AAI36235.1.
RefSeqiNP_001096549.1. NM_001103079.1.
UniGeneiStr.53387.

Genome annotation databases

GeneIDi100125194.
KEGGixtr:100125194.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC136234 mRNA. Translation: AAI36235.1.
RefSeqiNP_001096549.1. NM_001103079.1.
UniGeneiStr.53387.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100125194.
KEGGixtr:100125194.

Organism-specific databases

CTDi55290.
XenbaseiXB-GENE-964408. brf2.

Phylogenomic databases

HOVERGENiHBG107537.
InParanoidiA4QNR3.
KOiK15197.

Family and domain databases

Gene3Di1.10.472.10. 1 hit.
InterProiIPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERiPTHR11618. PTHR11618. 1 hit.
PfamiPF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 1 hit.
PROSITEiPS51134. ZF_TFIIB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiBRF2_XENTR
AccessioniPrimary (citable) accession number: A4QNR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 19, 2008
Last sequence update: May 14, 2007
Last modified: March 31, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.