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Protein

Ubiquitin carboxyl-terminal hydrolase 30

Gene

usp30

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Deubiquitinating enzyme that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei94NucleophilePROSITE-ProRule annotation1
Active sitei455Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-XTR-5689880. Ub-specific processing proteases.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 30 (EC:3.4.19.12By similarity)
Alternative name(s):
Deubiquitinating enzyme 30
Ubiquitin thioesterase 30
Ubiquitin-specific-processing protease 30
Short name:
Ub-specific protease 30
Gene namesi
Name:usp30
ORF Names:TEgg099b09.1
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
Proteomesi
  • UP000008143 Componenti: Unassembled WGS sequence

Organism-specific databases

XenbaseiXB-GENE-981069. usp30.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 52Mitochondrial intermembraneSequence analysisAdd BLAST52
Transmembranei53 – 73HelicalSequence analysisAdd BLAST21
Topological domaini74 – 519CytoplasmicSequence analysisAdd BLAST446

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003775391 – 519Ubiquitin carboxyl-terminal hydrolase 30Add BLAST519

Proteomic databases

PaxDbiA4QNN3.

Expressioni

Gene expression databases

BgeeiENSXETG00000022301.

Interactioni

Protein-protein interaction databases

STRINGi8364.ENSXETP00000048254.

Structurei

3D structure databases

ProteinModelPortaliA4QNN3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini85 – 504USPAdd BLAST420

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1867. Eukaryota.
ENOG410XQQ0. LUCA.
GeneTreeiENSGT00550000075075.
HOGENOMiHOG000065744.
InParanoidiA4QNN3.
KOiK11851.
OMAiCNETTTH.
OrthoDBiEOG091G0D1P.
TreeFamiTF105781.

Family and domain databases

InterProiView protein in InterPro
IPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
PfamiView protein in Pfam
PF00443. UCH. 1 hit.
PROSITEiView protein in PROSITE
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.

Sequencei

Sequence statusi: Complete.

A4QNN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWAPVSTWS RRTPLAACCS APELPPAGAW KACAAGSLRI GPQGRCKMMK
60 70 80 90 100
NWGMIGGIAA ALAAGIYVLW GPISDRKKYR KGLVPGLLNL GNTCFMNSLL
110 120 130 140 150
QGLASCPSFI RWLADFTSKY RQENNTTEHQ HLSVTLLHLL KALCNQEGTE
160 170 180 190 200
DEVLDASPLL EVLRAHRWQI SSFEEQDAHE LFHVLTSSLE DERDRRPHVT
210 220 230 240 250
HLFDLDSLEF PLEPQRQIHC RTQVPIYPIP SQWKSQHPFH GRLTSNMVCK
260 270 280 290 300
HCQHQSPMRY DTFDSLSLSI PVATWGHPIT LDQCLQHFIS TESVKDVVCE
310 320 330 340 350
NCTKIHAAQI PNSQSVENRK TTFVKQLKLG KLPQCLCIHL QRLSWSNQGS
360 370 380 390 400
PLKRNEHVQF SEFLAMDRFK YRISGCSTSK QPANHLSAAE QETTDGKEGG
410 420 430 440 450
AQNPTMPFLN GACSTSYISP PFTSPLPTNP EWTSSSYLFR LMAVVVHHGD
460 470 480 490 500
MHSGHFVTYR RSPAAKNQKL TSQQWLWISD DTVRRTNFQE VLSSSAYLLF
510
YERIQSNLHH PEDQRAAEK
Length:519
Mass (Da):58,683
Last modified:May 15, 2007 - v1
Checksum:i31FC4F6B8309D7E7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61A → V in CAJ81380 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR926280 mRNA. Translation: CAJ81380.1.
BC135925 mRNA. Translation: AAI35926.1.
RefSeqiNP_001039085.1. NM_001045620.1.
UniGeneiStr.19890.

Genome annotation databases

EnsembliENSXETT00000048254; ENSXETP00000048254; ENSXETG00000022301.
GeneIDi733894.
KEGGixtr:733894.

Similar proteinsi

Entry informationi

Entry nameiUBP30_XENTR
AccessioniPrimary (citable) accession number: A4QNN3
Secondary accession number(s): Q28CN8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: May 15, 2007
Last modified: September 27, 2017
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families