A4QNN3 (UBP30_XENTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 30
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 30
Ubiquitin thioesterase 30
Ubiquitin-specific-processing protease 30
Short name=Ub-specific protease 30

Gene names

Name:	usp30
ORF Names:	TEgg099b09.1

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	519 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May participate in the maintenance of mitochondrial morphology By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subcellular location	Mitochondrion outer membrane By similarity.
Sequence similarities	Belongs to the peptidase C19 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 519	519	Ubiquitin carboxyl-terminal hydrolase 30		PRO_0000377539
Regions
	Topological domain	1 – 52	52	Mitochondrial intermembrane Potential
	Transmembrane	53 – 73	21	Helical; Potential
	Topological domain	74 – 519	446	Cytoplasmic Potential
Sites
	Active site	94	1	Nucleophile By similarity
	Active site	455	1	Proton acceptor By similarity
Experimental info
	Sequence conflict	61	1	A → V in CAJ81380. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	A4QNN3 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 31FC4F6B8309D7E7	FASTA	519	58,683
	10 20 30 40 50 60 MSWAPVSTWS RRTPLAACCS APELPPAGAW KACAAGSLRI GPQGRCKMMK NWGMIGGIAA 70 80 90 100 110 120 ALAAGIYVLW GPISDRKKYR KGLVPGLLNL GNTCFMNSLL QGLASCPSFI RWLADFTSKY 130 140 150 160 170 180 RQENNTTEHQ HLSVTLLHLL KALCNQEGTE DEVLDASPLL EVLRAHRWQI SSFEEQDAHE 190 200 210 220 230 240 LFHVLTSSLE DERDRRPHVT HLFDLDSLEF PLEPQRQIHC RTQVPIYPIP SQWKSQHPFH 250 260 270 280 290 300 GRLTSNMVCK HCQHQSPMRY DTFDSLSLSI PVATWGHPIT LDQCLQHFIS TESVKDVVCE 310 320 330 340 350 360 NCTKIHAAQI PNSQSVENRK TTFVKQLKLG KLPQCLCIHL QRLSWSNQGS PLKRNEHVQF 370 380 390 400 410 420 SEFLAMDRFK YRISGCSTSK QPANHLSAAE QETTDGKEGG AQNPTMPFLN GACSTSYISP 430 440 450 460 470 480 PFTSPLPTNP EWTSSSYLFR LMAVVVHHGD MHSGHFVTYR RSPAAKNQKL TSQQWLWISD 490 500 510 DTVRRTNFQE VLSSSAYLLF YERIQSNLHH PEDQRAAEK

References

[1]	Sanger Xenopus tropicalis EST/cDNA project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Egg.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Tadpole.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR926280 mRNA. Translation: CAJ81380.1. BC135925 mRNA. Translation: AAI35926.1.
RefSeq	NP_001039085.1. NM_001045620.1.
UniGene	Str.19890.
3D structure databases
ProteinModelPortal	A4QNN3.
ModBase	Search...
Protein-protein interaction databases
STRING	8364.ENSXETP00000048254.
Protein family/group databases
MEROPS	C19.060.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSXETT00000048254; ENSXETP00000048254; ENSXETG00000022301.
GeneID	733894.
KEGG	xtr:733894.
Organism-specific databases
CTD	84749.
Xenbase	XB-GENE-981069. usp30.
Phylogenomic databases
eggNOG	COG5533.
GeneTree	ENSGT00550000075075.
HOGENOM	HOG000065744.
KO	K11851.
OMA	KTECTIM.
OrthoDB	EOG451DQH.
Gene expression databases
Bgee	A4QNN3.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBP30_XENTR

Accession

Primary (citable) accession number: A4QNN3
Secondary accession number(s): Q28CN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	May 15, 2007
Last modified:	April 3, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program