A4QK75 (NU1C_ARAHI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NAD(P)H-quinone oxidoreductase subunit 1, chloroplastic
EC=1.6.5.-

Alternative name(s):
NAD(P)H dehydrogenase subunit 1
Short name=NDH subunit 1
NADH-plastoquinone oxidoreductase subunit 1

Gene names

Name:

ndhA

Encoded on

Plastid; Chloroplast

Organism

Arabis hirsuta (Hairy rock-cress)

Taxonomic identifier

78191 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabideae › Arabis

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_01350
Catalytic activity	NAD(P)H + plastoquinone = NAD(P)+ + plastoquinol. HAMAP MF_01350
Subunit structure	NDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus By similarity.
Subcellular location	Plastid › chloroplast thylakoid membrane; Multi-pass membrane protein By similarity HAMAP MF_01350.
Sequence similarities	Belongs to the complex I subunit 1 family.

Ontologies

Keywords
Cellular component	Chloroplast Membrane Plastid Thylakoid
Domain	Transmembrane Transmembrane helix
Ligand	NAD NADP Plastoquinone
Molecular function	Oxidoreductase
PTM	Quinone
Gene Ontology (GO)
Cellular component	chloroplast thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	NAD(P)H-quinone oxidoreductase subunit 1, chloroplastic HAMAP MF_01350		PRO_0000298865
Regions
	Transmembrane	27 – 47	21	Helical; Potential
	Transmembrane	98 – 118	21	Helical; Potential
	Transmembrane	129 – 149	21	Helical; Potential
	Transmembrane	165 – 185	21	Helical; Potential
	Transmembrane	203 – 223	21	Helical; Potential
	Transmembrane	253 – 273	21	Helical; Potential
	Transmembrane	302 – 322	21	Helical; Potential
	Transmembrane	345 – 365	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	A4QK75 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: A031A3C56179FA97	FASTA	365	40,683
	10 20 30 40 50 60 MIIYATEVET INSFVRLESL KELYSLVWIF VPIFSLVLGI ITGVLVIAWI ERELSAGIQQ 70 80 90 100 110 120 RIGPEYAGPL GILQALADGT KLLFKEDLRP SRGNTPLFSI GPSIAVISIL LSYSVIPFSN 130 140 150 160 170 180 HLVLADLNIG IFLWIAISSI APIGLLMSGY GSNNKYSFLG GLRAAAQSIS YEIPLTLCVL 190 200 210 220 230 240 SISLLSNSLS TVDIVEAQSK YGFWGWNLWR QPIGFIIFLI SSLAECERLP FDLPEAEEEL 250 260 270 280 290 300 IAGYQTEYSG IKFGLFYVAS YINLLISSLF VTILYLGGWN ISIPYISIPY ISILELFERD 310 320 330 340 350 360 PIFGTTIGIF ITLAKTYLFL FISIATRWTL PRLRMDQLLN LGWKFLLPIS LGNLLLTTSF QLFSL

References

[1]	"Sequencing analysis of Arabis hirsuta chloroplast DNA." Hosouchi T., Tsuruoka H., Kotani H. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JO23.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009369 Genomic DNA. Translation: BAF50080.1.
RefSeq	YP_001123255.1. NC_009268.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4962577.
Phylogenomic databases
ProtClustDB	CHL00032.
Family and domain databases
HAMAP	MF_01350. NDH1_NuoH. [Tree]
InterPro	IPR001694. NADH_UbQ_OxRdtase_su1/FPO. IPR018086. NADH_UbQ_OxRdtase_su1_CS. [Graphical view]
PANTHER	PTHR11432. Resp_NADH_DH_1. 1 hit.
Pfam	PF00146. NADHdh. 1 hit. [Graphical view]
PROSITE	PS00667. COMPLEX1_ND1_1. 1 hit. PS00668. COMPLEX1_ND1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NU1C_ARAHI

Accession

Primary (citable) accession number: A4QK75

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	May 15, 2007
Last modified:	June 28, 2011
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program