A4QJE0 (CLPP_AETCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent Clp protease proteolytic subunit
EC=3.4.21.92

Alternative name(s):
Endopeptidase Clp

Gene names

Name:

clpP

Encoded on

Plastid; Chloroplast

Organism

Aethionema cordifolium (Lebanon stonecress)

Taxonomic identifier

434059 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Aethionemeae › Aethionema

Protein attributes

Sequence length	196 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP-Rule MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP-Rule MF_00444
Subunit structure	Component of the chloroplastic Clp protease core complex.
Subcellular location	Plastid › chloroplast stroma By similarity.
Sequence similarities	Belongs to the peptidase S14 family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 196	196	ATP-dependent Clp protease proteolytic subunit HAMAP-Rule MF_00444		PRO_0000309286
Sites
	Active site	101	1	By similarity
	Active site	126	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4QJE0 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 2A0A3FDA4EFAE709	FASTA	196	22,070
	10 20 30 40 50 60 MPIGVPKVPF RSPGEGDTSW VDIYNRLYRE RLFFLGQEVD TDISNQLISL MIYLSIEKDT 70 80 90 100 110 120 KDLYLFINSP GGWVISGMAI YDTMQFVRPD VQTICMGLAA SIASFILVGG AITKRIAFPH 130 140 150 160 170 180 ARVMIHQPAS SFYEAQTGEF ILEAEELLKL RETITRVYVQ RTGKPIWVVS EDMERDVFMS 190 ATEAQAHGIV DLVAVQ

References

[1]	"Sequencing analysis of Aethionema coridifolium chloroplast DNA." Hosouchi T., Tsuruoka H., Kotani H. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009366 Genomic DNA. Translation: BAF49795.1.
RefSeq	YP_001122971.1. NC_009265.1.
3D structure databases
ProteinModelPortal	A4QJE0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4968625.
Phylogenomic databases
ProtClustDB	CHL00028.
Family and domain databases
HAMAP	MF_00444. ClpP.
InterPro	IPR001907. ClpP. IPR023562. ClpP/TepA. IPR018215. ClpP_AS. [Graphical view]
PANTHER	PTHR10381. PTHR10381. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. PS00381. CLP_PROTEASE_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPP_AETCO

Accession

Primary (citable) accession number: A4QJE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	May 15, 2007
Last modified:	April 3, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program