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A4QI59 (SYL_CORGB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:cgR_2905
OrganismCorynebacterium glutamicum (strain R) [Complete proteome] [HAMAP]
Taxonomic identifier340322 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length952 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 952952Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009333

Regions

Motif66 – 7712"HIGH" region HAMAP-Rule MF_00049
Motif722 – 7265"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site7251ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4QI59 [UniParc].

Last modified May 15, 2007. Version 1.
Checksum: CA1A789CF8EAE151

FASTA952106,747
        10         20         30         40         50         60 
MTNPSEGTTP LAFRYTPELA NKIEGEWQNY WTDNGTFNAP NPVGDLAPAD GKALPEDKLF 

        70         80         90        100        110        120 
VQDMFPYPSG AGLHVGHPLG YIATDVFARY NRMLGKNVLH TLGYDAFGLP AEQYAIQTGT 

       130        140        150        160        170        180 
HPRTTTMANI ENMKRQLGAL GLGHDSRRAV ATTDPEFYKW TQWIFLQIFN SWFDAEQQKA 

       190        200        210        220        230        240 
RPISELIPLL ESGELKTKDG ADYNALGDVE KQKAVDDYRL VYRSNSTVNW CPGLGTVLAN 

       250        260        270        280        290        300 
EEVTADGRSE RGNFPVFRKN LSQWMMRITA YSDRLIDDLE LLDWTEKVKS MQRNWIGRSR 

       310        320        330        340        350        360 
GAEVDFSAEG ETVTVFTTRP DTLFGATYMV LAPEHELVDV LLEKAGSYEG VDARWTNGQA 

       370        380        390        400        410        420 
SPAEAVAAYR ASIAAKSDLE RQENKEKTGV FLGVYATNPV NGDQIPVFIA DYVLTGYGTG 

       430        440        450        460        470        480 
AIMAVPAHDE RDYEFATVLG LPIKEVVAGG NIEEAAFTES GEAVNSANDN GLDINGLAKD 

       490        500        510        520        530        540 
EAVAKTIEWL EEKELGRGTI QYKLRDWLFA RQRYWGEPFP IVYDENGQAH ALPDSMLPVE 

       550        560        570        580        590        600 
LPEVEDYKPV SFDPEDADSE PSPPLAKARE WVEVELDLGD GKKKYTRDTN VMPQWAGSSW 

       610        620        630        640        650        660 
YQLRYVDPSN DEQFCNIENE RYWTGPRPET HGPNDPGGVD LYVGGVEHAV LHLLYARFWH 

       670        680        690        700        710        720 
KVLFDLGHVS SKEPYRRLYN QGYIQAFAYT DSRGVYVPAD DVEEKDGKFF YQGEEVNQEY 

       730        740        750        760        770        780 
GKMGKSLKNA VAPDDICNNF GADTLRVYEM AMGPLDTSRP WATKDVVGAQ RFLQRLWRLV 

       790        800        810        820        830        840 
VDENTGEVLT RDEVLTDDDN KQLHRTIAGV RDDYTNLRVN TVVAKLIEYV NYLTKTYPDT 

       850        860        870        880        890        900 
IPAGAVLPLI VMVSPVAPHI AEELWKKLGH DDTVTYEPFP TFEEKWLTDD EIELPVQVNG 

       910        920        930        940        950 
KVRGRITVAA DASQEQVIEA ALADEKVQEQ ISGKNLIKQI VVPGRMVNLV VK 

« Hide

References

[1]"Comparative analysis of the Corynebacterium glutamicum group and complete genome sequence of strain R."
Yukawa H., Omumasaba C.A., Nonaka H., Kos P., Okai N., Suzuki N., Suda M., Tsuge Y., Watanabe J., Ikeda Y., Vertes A.A., Inui M.
Microbiology 153:1042-1058(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: R.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009044 Genomic DNA. Translation: BAF55925.1.
RefSeqYP_001139827.1. NC_009342.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING340322.cgR_2905.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAF55925; BAF55925; cgR_2905.
GeneID4993659.
KEGGcgt:cgR_2905.
PATRIC21510514. VBICorGlu58097_2975.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAREKNDWE.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycCGLU340322:GJBE-3041-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_CORGB
AccessionPrimary (citable) accession number: A4QI59
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 15, 2007
Last modified: April 16, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries