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A4QHQ6 (TRMB_CORGB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:cgR_2753
OrganismCorynebacterium glutamicum (strain R) [Complete proteome] [HAMAP]
Taxonomic identifier340322 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length255 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 255255tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_1000064390

Regions

Region164 – 1696Interaction with RNA Potential
Region232 – 2354Substrate binding By similarity

Sites

Active site1581 By similarity
Binding site831S-adenosyl-L-methionine By similarity
Binding site1081S-adenosyl-L-methionine By similarity
Binding site1351S-adenosyl-L-methionine By similarity
Binding site1581S-adenosyl-L-methionine By similarity
Binding site1621Substrate By similarity
Binding site1941Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A4QHQ6 [UniParc].

Last modified May 15, 2007. Version 1.
Checksum: 550F5003FAE01AA6

FASTA25528,806
        10         20         30         40         50         60 
MSISDNSRDQ LGELPAGRPL QSDFDNDLDY PRLGSVTFRR GTLTENQQTM WDEKWPELGR 

        70         80         90        100        110        120 
VLEDELIDVD AWFGREGAKT IVEIGSGTGT STAAMAPLEA DTNIVAVELY KPGLAKLMGS 

       130        140        150        160        170        180 
VVRGEIDNVR MVRGDGIEVL NRMFADGSLD GIRVYFPDPW PKARHNKRRI IQSGPLNLFA 

       190        200        210        220        230        240 
KKLKPGGVLH VATDHADYAE WINELVEVEP LLEYKGWPWE ECPQLTDRQV ITKFEGKGLE 

       250 
KDHVINEYLW QKVQN 

« Hide

References

[1]"Comparative analysis of the Corynebacterium glutamicum group and complete genome sequence of strain R."
Yukawa H., Omumasaba C.A., Nonaka H., Kos P., Okai N., Suzuki N., Suda M., Tsuge Y., Watanabe J., Ikeda Y., Vertes A.A., Inui M.
Microbiology 153:1042-1058(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: R.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009044 Genomic DNA. Translation: BAF55772.1.
RefSeqYP_001139674.1. NC_009342.1.

3D structure databases

ProteinModelPortalA4QHQ6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING340322.cgR_2753.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAF55772; BAF55772; cgR_2753.
GeneID4994270.
KEGGcgt:cgR_2753.
PATRIC21510198. VBICorGlu58097_2820.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMATEILQDC.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycCGLU340322:GJBE-2884-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_CORGB
AccessionPrimary (citable) accession number: A4QHQ6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 15, 2007
Last modified: April 16, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways