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A4QHQ4 (PCKG_CORGB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [GTP]

Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.32
Gene names
Name:pckG
Ordered Locus Names:cgR_2751
OrganismCorynebacterium glutamicum (strain R) [Complete proteome] [HAMAP]
Taxonomic identifier340322 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length610 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity. HAMAP-Rule MF_00452

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP-Rule MF_00452

Cofactor

Binds 1 manganese ion per subunit By similarity. HAMAP-Rule MF_00452

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00452

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00452

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00452.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandGTP-binding
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

phosphoenolpyruvate carboxykinase (GTP) activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 610610Phosphoenolpyruvate carboxykinase [GTP] HAMAP-Rule MF_00452
PRO_1000060288

Regions

Nucleotide binding273 – 2786GTP By similarity
Nucleotide binding515 – 5184GTP By similarity
Region387 – 3893Substrate binding By similarity

Sites

Active site2741 By similarity
Metal binding2301Manganese By similarity
Metal binding2501Manganese; via tele nitrogen By similarity
Metal binding2971Manganese By similarity
Binding site821Substrate By similarity
Binding site2231Substrate; via amide nitrogen By similarity
Binding site2301Substrate By similarity
Binding site2721Substrate By similarity
Binding site3891GTP By similarity
Binding site4201GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4QHQ4 [UniParc].

Last modified May 15, 2007. Version 1.
Checksum: 7958DBA2E99B0F30

FASTA61066,861
        10         20         30         40         50         60 
MTTAAIRGLQ GEAPTKNKEL LNWIADAVEL FQPEAVVFVD GSQAEWDRMA EDLVEAGTLI 

        70         80         90        100        110        120 
KLNEEKRPNS YLARSNPSDV ARVESRTFIC SEKEEDAGPT NNWAPPQAMK DEMSKHYAGS 

       130        140        150        160        170        180 
MKGRTMYVVP FCMGPISDPD PKLGVQLTDS EYVVMSMRIM TRMGIEALDK IGANGSFVKC 

       190        200        210        220        230        240 
LHSVGAPLEP GQEDVAWPCN DTKYITQFPE TKEIWSYGSG YGGNAILAKK CYALRIASVM 

       250        260        270        280        290        300 
AREEGWMAEH MLILKLINPE GKAYHIAAAF PSACGKTNLA MITPTIPGWT AQVVGDDIAW 

       310        320        330        340        350        360 
LKLREDGLYA VNPENGFFGV APGTNYASNP IAMKTMEPGN TLFTNVALTD DGDIWWEGMD 

       370        380        390        400        410        420 
GDAPAHLIDW KGNDWTPESD ENAAHPNSRY CVAIDQSPAA APEFNDWEGV KIDAILFGGR 

       430        440        450        460        470        480 
RADTVPLVTQ TYDWEHGTMV GALLASGQTA ASAEAKVGTL RHDPMAMLPF IGYNAGEYLQ 

       490        500        510        520        530        540 
NWIDMGNKGG DKMPSIFLVN WFRRGEDGRF LWPGFGDNSR VLKWVIDRIE GRVGADETVV 

       550        560        570        580        590        600 
GHTAKAEDLD LDGLDTPIED VKEALTAPAE QWANDVQDNA EYLTFLGPRV PAEVHSQFDA 

       610 
LKARISAAHA 

« Hide

References

[1]"Comparative analysis of the Corynebacterium glutamicum group and complete genome sequence of strain R."
Yukawa H., Omumasaba C.A., Nonaka H., Kos P., Okai N., Suzuki N., Suda M., Tsuge Y., Watanabe J., Ikeda Y., Vertes A.A., Inui M.
Microbiology 153:1042-1058(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: R.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009044 Genomic DNA. Translation: BAF55770.1.
RefSeqYP_001139672.1. NC_009342.1.

3D structure databases

ProteinModelPortalA4QHQ4.
SMRA4QHQ4. Positions 13-606.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING340322.cgR_2751.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAF55770; BAF55770; cgR_2751.
GeneID4992267.
KEGGcgt:cgR_2751.
PATRIC21510194. VBICorGlu58097_2818.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1274.
HOGENOMHOG000191700.
KOK01596.
OMAWMRFGED.
OrthoDBEOG6MPWQS.
ProtClustDBPRK04210.

Enzyme and pathway databases

BioCycCGLU340322:GJBE-2882-MONOMER.
UniPathwayUPA00138.

Family and domain databases

Gene3D3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPMF_00452. PEPCK_GTP.
InterProIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERPTHR11561. PTHR11561. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMSSF68923. SSF68923. 1 hit.
PROSITEPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCKG_CORGB
AccessionPrimary (citable) accession number: A4QHQ4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 15, 2007
Last modified: April 16, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways