A4QFJ0 (A4QFJ0_CORGB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malto-oligosyltrehalose trehalohydrolase PIRNR PIRNR006337
Short name=MTHase PIRNR PIRNR006337
EC=3.2.1.141 PIRNR PIRNR006337

Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase PIRNR PIRNR006337
Maltooligosyl trehalose trehalohydrolase PIRNR PIRNR006337

Gene names

Ordered Locus Names:

cgR_2009

Organism

Corynebacterium glutamicum (strain R) [Complete proteome] [HAMAP]

Taxonomic identifier

340322 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	610 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan. PIRNR PIRNR006337
Pathway	Glycan biosynthesis; trehalose biosynthesis. PIRNR PIRNR006337
Subcellular location	Cytoplasm By similarity PIRSR PIRSR006337-1.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. PIRNR PIRNR006337

Ontologies

Keywords
Molecular function	Glycosidase PIRNR PIRNR006337 Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	285	1	Nucleophile By similarity PIRSR PIRSR006337-1
	Active site	322	1	Proton donor By similarity PIRSR PIRSR006337-1
	Site	418	1	Transition state stabilizer By similarity PIRSR PIRSR006337-3

Sequences

	Sequence		Length	Mass (Da)
	A4QFJ0 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: F993339CCC79D70B	FASTA	610	67,601
	10 20 30 40 50 60 MLKDSIGLRE LILREMCHSI SHLNPPSLSI FANLVAMLTS QSFSVWAPLP KDVQLILNGE 70 80 90 100 110 120 TFPMTKTEGS WWRAEIAPKA GDRYGFSLFD GSSWSKPLPD PRSTSQPDGV HGLSEVSEDS 130 140 150 160 170 180 YLWGDQQWTG RILPGSVLYE LHVGTFSEDG TFEGVVDKLP YLRDLGVTAI ELLPVQPFGG 190 200 210 220 230 240 NRNWGYDGVL WHAVHAGYGG PAGLKKLIDA SHQAGIAVYL DVVYNHFGPD GNYNGQFGPY 250 260 270 280 290 300 TSGGSTGWGD VVNINGHDSD EVRNYILDAA RQWFEDFHVD GLRLDAVHSL DDRGAYSLLA 310 320 330 340 350 360 QLTMVAEDVS AQTGIPRSLI AESELNDPKL VTSREAGGFG LDAQWVDDIH HALHALVSGE 370 380 390 400 410 420 RNGYYSDFGS VDTLAKTLRE VFEHTGNYST YRGRNHGRPV HPDITPASRF VTYTTTHDQT 430 440 450 460 470 480 GNRAIGDRPS MTLTPEQQVL KAAIIYSSPY TPMLFMGEEF GATTPFAFFC SHTDPELNRL 490 500 510 520 530 540 TSEGRKREFA RLGWNADDIP SPELESTFTS SKLDWEFTAE QRRINDAYKQ LLHLRHTLGF 550 560 570 580 590 600 SQPNLLTLEV EHGENWLSMA NGRGRILANF SDDTITVPFG GELIYSFTSP TVTDTSTTLQ 610 PWGFAILTRN

References

[1]

"Comparative analysis of the Corynebacterium glutamicum group and complete genome sequence of strain R." Yukawa H., Omumasaba C.A., Nonaka H., Kos P., Okai N., Suzuki N., Suda M., Tsuge Y., Watanabe J., Ikeda Y., Vertes A.A., Inui M. Microbiology 153:1042-1058(2007) [PubMed: 17379713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: R.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009044 Genomic DNA. Translation: BAF55006.1.
RefSeq	YP_001138908.1. NC_009342.1.
3D structure databases
ProteinModelPortal	A4QFJ0.
ModBase	Search...
Protein-protein interaction databases
STRING	A4QFJ0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4992429.
GenomeReviews	Gene locus cgR_2009 in contig AP009044_GR.
KEGG	cgt:cgR_2009.
PATRIC	21508646. VBICorGlu58097_2069.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HBG367595.
OMA	EGFVYQG.
ProtClustDB	CLSK638715.
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR006047. Glyco_hydro_13_cat_dom. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR012768. Trehalose_TreZ. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K01236.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF21. PTHR10357:SF21. 1 hit.
Pfam	PF00128. Alpha-amylase. 2 hits. [Graphical view]
PIRSF	PIRSF006337. Trehalose_TreZ. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR02402. Trehalose_TreZ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A4QFJ0_CORGB

Accession

Primary (citable) accession number: A4QFJ0

Entry history

Integrated into UniProtKB/TrEMBL:	May 15, 2007
Last sequence update:	May 15, 2007
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)