A4QF01 (SYP_CORGB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	cgR_1823

Organism

Corynebacterium glutamicum (strain R) [Complete proteome] [HAMAP]

Taxonomic identifier

340322 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity. HAMAP MF_01569
Subcellular location	Cytoplasm By similarity HAMAP MF_01569.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 588	588	Proline--tRNA ligase HAMAP MF_01569		PRO_1000069134

Sequences

	Sequence		Length	Mass (Da)
	A4QF01 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 362F7960D2C8012B	FASTA	588	64,350
	10 20 30 40 50 60 MITRLSTLFL RTLREDPADA EVPSHKLLVR AGYIRRVAPG IYSWLPLGLR AVRNIEAVVR 70 80 90 100 110 120 EEMDAIGGQE LLFPALLPRE PYETTQRWTE YGDSLFRLKD RKGADYLLGP THEEMFAATV 130 140 150 160 170 180 KDLYNSYKDF PVTLYQIQTK YRDEERPRAG VLRGREFVMK DSYSFDMSDA GLDESYAKHR 190 200 210 220 230 240 AAYQRIFDRL GLEYAICQAT SGAMGGSASE EFLAVSENGE DTFVRSTSGN YAANVEAVVT 250 260 270 280 290 300 QPGVERDIEG LPEAVTYETP VSETIDALVD WANSIDVQIE GREVTAADTL KCIVVKVREP 310 320 330 340 350 360 GAEEAELTGI LLPGDREVDM KRLEASLEPA EVELAVESDF ADNPFLVKGY VGPVGLAKNG 370 380 390 400 410 420 VKVLADPRVV TGTSWITGAD EKERHVVGLV AGRDFTPDGF IEAAEIKEGD PAPAGEGTLT 430 440 450 460 470 480 LARGIEIGHI FQLGRKYTEA FDVQILDENG KRAIPTMGSY GLGVTRLLAV LAEQRHDDAG 490 500 510 520 530 540 LNWSVEVAPY QVHVVAANKD AAAIEAAERF AAELSAAGLD VLFDDRPKVS PGVKFKDAEL 550 560 570 580 LGMPFALILG RGYAEGKVEL RVRGGEKSEL DADQAVAQIV EMVAQARN

References

[1]

"Comparative analysis of the Corynebacterium glutamicum group and complete genome sequence of strain R." Yukawa H., Omumasaba C.A., Nonaka H., Kos P., Okai N., Suzuki N., Suda M., Tsuge Y., Watanabe J., Ikeda Y., Vertes A.A., Inui M. Microbiology 153:1042-1058(2007) [PubMed: 17379713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: R.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009044 Genomic DNA. Translation: BAF54817.1.
RefSeq	YP_001138719.1. NC_009342.1.
3D structure databases
ProteinModelPortal	A4QF01.
ModBase	Search...
Protein-protein interaction databases
STRING	A4QF01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4991919.
GenomeReviews	Gene locus cgR_1823 in contig AP009044_GR.
KEGG	cgt:cgR_1823.
PATRIC	21508260. VBICorGlu58097_1877.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
HOGENOM	HBG403504.
OMA	IQPAELW.
ProtClustDB	PRK09194.
Family and domain databases
HAMAP	MF_01569. Pro_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023717. Pro-tRNA-Synthase_IIa_type1. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.90.960.10. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF55826. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
TIGRFAMs	TIGR00409. ProS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_CORGB

Accession

Primary (citable) accession number: A4QF01

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 15, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program